LOCUS       sequence-418901-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human CXCR4-E4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(CXCR4-E4(56))-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        234..432
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     387..407
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             450..630
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    677..802
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1301..1534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1719..1763
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2061..2178
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2253..2501
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2253..2273
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2424..2443
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2630..3129
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2665..2688)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3062..3081
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3169..3765
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3169..3188)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3665..3685
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3784..3837
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3838..4539
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4599..4619)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5017..5028)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5480..5497)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5486..5710
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5736..5756)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5736..5754)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5775..5791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5775..5791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5788..5810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5799..5815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5823..5829,5830..5847,5848..5853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5868..5889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6006..6023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6177..6765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6257..6276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6936..7727,7728..7796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7559..7578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7797..7901)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7927..8382)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8064..8085)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8276..8295
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8509..8531
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8523..8540
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8524..8540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8550..8569
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8550..8568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
       61 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      121 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      181 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      241 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      301 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      361 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      421 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      481 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      541 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      601 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      661 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      721 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      781 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      841 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
      901 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
      961 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1021 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1081 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1141 ggccggccgc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1201 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1261 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1321 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1381 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1441 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1501 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1561 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1621 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1681 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1741 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1801 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     1861 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     1921 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     1981 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     2041 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2101 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2161 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta
     2221 ccgggcccta cgcgtccaag gtcgggcagg aagagggcct atttcccatg attccttcat
     2281 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     2341 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2401 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2461 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtcattaaa atgctgttct
     2521 ggtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     2581 cttgaaaaag tggcaccgag tcggtgcttt ttttggatcc aattctaccg ggtaggggag
     2641 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2701 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2761 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2821 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2881 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2941 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     3001 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     3061 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     3121 ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat gaccgagtac
     3181 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3241 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3301 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3361 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     3421 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3481 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3541 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3601 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3661 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3721 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggcgg cgggtccgga
     3781 ggagagggca gaggaagtct cctaacatgc ggtgacgtgg aggagaatcc tggcccaatg
     3841 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
     3901 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     3961 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
     4021 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
     4081 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgagga cgggggcgtg
     4141 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     4201 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
     4261 gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
     4321 ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag
     4381 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
     4441 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
     4501 tgcgacctcc ctagcaaact ggggcacaag cttaattgag cggccgctca acctctggat
     4561 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4621 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4681 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4741 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4801 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4861 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4921 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4981 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5041 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctacgcct tcgccctcag
     5101 acgagtcgga tctccctttg ggccgcctcc ccgcaggtac ctttaagacc aatgacttac
     5161 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5221 cactcccaaa gaagtcaaga tctgcttttt gcctgtactg ggtctctctg gttagaccag
     5281 agtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5341 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5401 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5461 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5521 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5581 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5641 gggcaggaca gcaaggggga ggattgggaa gtcaatagca ggcatgctgg ggatgcggtg
     5701 ggctctatgg gcggccgtta attaagcttt tgttcccttt agtgagggtt aattgcgcgc
     5761 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5821 cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5881 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5941 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6001 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7921 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     7981 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8041 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8101 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8161 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8221 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8281 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8341 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     8401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     8461 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     8521 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgacta
     8581 gtacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     8641 cgtcaataat
//
