LOCUS       sequence-418933-        8334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Renz C, Asimaki E, Meister C, Albanese V, Petriukov K, Krapoth NC, 
            Wegmann S, Wollscheid HP, Wong RP, Fulzele A, Chen JX, Leon S, 
            Ulrich HD
  TITLE     Ubiquiton-An inducible, linkage-specific polyubiquitylation tool.
  JOURNAL   Mol Cell. 2024 Jan 18;84(2):386-400.e11. doi: 
            10.1016/j.molcel.2023.11.016. Epub 2023 Dec 15.
  PUBMED    38103558
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.11.016"; journalName: "Mol Cell"; date: 
            "2024-01-18- 18"; volume: "84"; issue: "2"; pages: "386-400.e11"
COMMENT     Sequence Label: pcDNA5-FRT-TO-EGFR*-CUbo-His6
FEATURES             Location/Qualifiers
     source          1..8334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3048..3050,3051..3371)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3507..3524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3528..4238
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3555..3583)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4178..4198
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4286..4303)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4292..4516
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4649..4668)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4800..4847
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4855..5877
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4855..5877
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6007..6128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6044..6063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6098..6117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6177..6193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6177..6193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6190..6212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6201..6217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6225..6231,6232..6249,6250..6255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6270..6291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6408..6425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6579..7167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6659..6678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7338..8129,8130..8198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7961..7980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8199..8303)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgcgac cctccgggac ggccggggca gcgctcctgg
     1021 cgctgctggc tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag
     1081 gcacgagtaa caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga
     1141 ggatgttcaa taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga
     1201 attatgatct ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc
     1261 tcaacacagt ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact
     1321 acgaaaattc ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga
     1381 aggagctgcc catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca
     1441 accctgccct gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc
     1501 tcagcaacat gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa
     1561 gctgtcccaa tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa
     1621 tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc
     1681 acaaccagtg tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca
     1741 aattccgaga cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca
     1801 ccacgtacca gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga
     1861 agaagtgtcc ccgtaattat gtggtgacag atcacggctc gtgcgtccga gcctgtgggg
     1921 ccgacagcta tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt
     1981 gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg
     2041 ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc
     2101 cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg
     2161 atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa
     2221 acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac
     2281 atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc
     2341 tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa
     2401 atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca
     2461 acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg
     2521 agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca
     2581 gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact
     2641 ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag
     2701 gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca
     2761 agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg
     2821 ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc
     2881 ttgaaggctg tccaacgaat gggcctaaga tcccatcgat agccactggg atggtggggg
     2941 ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca
     3001 tcgttcggaa gcgcacgctg cgtcgactgg gtaccgagct cggatccatg ggagtgcagg
     3061 tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgcgtgg
     3121 tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg gacagaaaca
     3181 agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg
     3241 cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg
     3301 ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc
     3361 taaaactgga agggtcgacc ggtatccctc cagatcaaca aagattgatc tttgccggta
     3421 agcagctaga agacggtaga acgctgtctg attacaacat tcagaaggag tccaccttac
     3481 atcttgtgct aaggctaaga ggtgttcatc accatcacca tcactaaagt aaaggagaag
     3541 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     3601 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     3661 ttatttgcac tactggaaaa ctacctgttc catggccaac actggtcacc actttcactt
     3721 atggtgttca atgcttctcg agatacccag accatatgaa acggcatgac tttttcaaga
     3781 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     3841 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     3901 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     3961 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gctaacttca
     4021 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     4081 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     4141 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     4201 ctgctgggat tacacatggc atggatgaac tatacaaata agcggccgct cgagtctaga
     4261 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     4321 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4381 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4441 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4501 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4561 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4621 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4681 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4741 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     4801 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     4861 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     4921 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     4981 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     5041 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     5101 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     5161 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     5221 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     5281 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     5341 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     5401 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     5461 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     5521 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     5581 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     5641 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     5701 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     5761 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     5821 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     5881 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5941 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6001 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6121 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6181 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6241 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6301 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
