LOCUS       sequence-418937-       11679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of 
            the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Nunez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, 
            Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung 
            A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, 
            Gilbert LA, Weissman JS
  TITLE     Genome-wide programmable transcriptional memory by CRISPR-based 
            epigenome editing.
  JOURNAL   Cell. 2021 Apr 7. pii: S0092-8674(21)00353-6. doi: 
            10.1016/j.cell.2021.03.025.
  PUBMED    33838111
REFERENCE   2  (bases 1 to 11679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 7. pii: S0092-8674(21)00353-6. doi: 10.1016/j.cell.2021.03.025."
COMMENT     Sequence Label: TETv4
FEATURES             Location/Qualifiers
     source          1..11679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     primer_bind     1116..1138
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1200..1219
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1250..1259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3656..3682,3683..3685,3686..6172,6173..6175,
                     6176..7759)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7775..7795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7799..7819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7823..7843
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7856..7912
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7913..8611
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     complement(8701..8720)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8766..8821
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8820..8839)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9182..9198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9182..9198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9195..9217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9206..9222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9230..9236,9237..9254,9255..9260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9275..9296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9355..9550
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9401..9536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9556..9690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9593..9612)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9647..9666
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9758..9775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9929..10517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10009..10028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10688..11479,11480..11548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11311..11330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11549..11653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1021 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1081 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1141 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1201 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattctgcg ccaccatggc
     1261 cctgcccacc tgcagctgtc ttgatcgagt tatacaaaaa gacaaaggcc catattatac
     1321 acaccttggg gcaggaccaa gtgttgctgc tgtcagggaa atcatggaga ataggtatgg
     1381 tcaaaaagga aacgcaataa ggatagaaat agtagtgtac accggtaaag aagggaaaag
     1441 ctctcatggg tgtccaattg ctaagtgggt tttaagaaga agcagtgatg aagaaaaagt
     1501 tctttgtttg gtccggcagc gtacaggcca ccactgtcca actgctgtga tggtggtgct
     1561 catcatggtg tgggatggca tccctcttcc aatggccgac cggctataca cagagctcac
     1621 agagaatcta aagtcataca atgggcaccc taccgacaga agatgcaccc tcaatgaaaa
     1681 tcgtacctgt acatgtcaag gaattgatcc agagacttgt ggagcttcat tctcttttgg
     1741 ctgttcatgg agtatgtact ttaatggctg taagtttggt agaagcccaa gccccagaag
     1801 atttagaatt gatccaagct ctcccttaca tgaaaaaaac cttgaagata acttacagag
     1861 tttggctaca cgattagctc caatttataa gcagtatgct ccagtagctt accaaaatca
     1921 ggtggaatat gaaaatgttg cccgagaatg tcggcttggc agcaaggaag gtcgaccctt
     1981 ctctggggtc actgcttgcc tggacttctg tgctcatccc cacagggaca ttcacaacat
     2041 gaataatgga agcactgtgg tttgtacctt aactcgagaa gataaccgct ctttgggtgt
     2101 tattcctcaa gatgagcagc tccatgtgct acctctttat aagctttcag acacagatga
     2161 gtttggctcc aaggaaggaa tggaagccaa gatcaaatct ggggccatcg aggtcctggc
     2221 accccgccgc aaaaaaagaa cgtgtttcac tcagcctgtt ccccgttctg gaaagaagag
     2281 ggctgcgatg atgacagagg ttcttgcaca taagataagg gcagtggaaa agaaacctat
     2341 tccccgaatc aagcggaaga ataactcaac aacaacaaac aacagtaagc cttcgtcact
     2401 gccaacctta gggagtaaca ctgagaccgt gcaacctgaa gtaaaaagtg aaaccgaacc
     2461 ccattttatc ttaaaaagtt cagacaacac taaaacttat tcgctgatgc catccgctcc
     2521 tcacccagtg aaagaggcat ctccaggctt ctcctggtcc ccgaagactg cttcagccac
     2581 accagctcca ctgaagaatg acgcaacagc ctcatgcggg ttttcagaaa gaagcagcac
     2641 tccccactgt acgatgcctt cgggaagact cagtggtgcc aatgctgcag ctgctgatgg
     2701 ccctggcatt tcacagcttg gcgaagtggc tcctctcccc accctgtctg ctcctgtgat
     2761 ggagcccctc attaattctg agccttccac tggtgtgact gagccgctaa cgcctcatca
     2821 gccaaaccac cagccctcct tcctcacctc tcctcaagac cttgcctctt ctccaatgga
     2881 agaagatgag cagcattctg aagcagatga gcctccatca gacgaacccc tatctgatga
     2941 ccccctgtca cctgctgagg agaaattgcc ccacattgat gagtattggt cagacagtga
     3001 gcacatcttt ttggatgcaa atattggtgg ggtggccatc gcacctgctc acggctcggt
     3061 tttgattgag tgtgcccggc gagagctgca cgctaccact cctgttgagc accccaaccg
     3121 taatcatcca acccgcctct cccttgtctt ttaccagcac aaaaacctaa ataagcccca
     3181 acatggtttt gaactaaaca agattaagtt tgaggctaaa gaagctaaga ataagaaaat
     3241 gaaggcctca gagcaaaaag accaggcagc taatgaaggt ccagaacagt cctctgaagt
     3301 aaatgaattg aaccaaattc cttctcataa agcattaaca ttaacccatg acaatgttgt
     3361 caccgtgtcc ccttatgctc tcacacacgt tgcggggccc tataaccatt gggtcggagg
     3421 gccgagctct ggcgcacccc caccaagtgg agggtctcct gccgggtccc caacatctac
     3481 tgaagaaggc accagcgaat ccgcaacgcc cgagtcaggc cctggtacct ccacagaacc
     3541 atctgaaggt agtgcgcctg gttccccagc tggaagccct acttccaccg aagaaggcac
     3601 gtcaaccgaa ccaagtgaag gatctgcccc tgggaccagc actgaaccat ctgagatgga
     3661 caagaagtat tctatcggac tggccatcgg gactaatagc gtcgggtggg ccgtgatcac
     3721 tgacgagtac aaggtgccct ctaagaagtt caaggtgctc gggaacaccg accggcattc
     3781 catcaagaaa aatctgatcg gagctctcct ctttgattca ggggagaccg ctgaagcaac
     3841 ccgcctcaag cggactgcta gacggcggta caccaggagg aagaaccgga tttgttacct
     3901 tcaagagata ttctccaacg aaatggcaaa ggtcgacgac agcttcttcc ataggctgga
     3961 agaatcattc ctcgtggaag aggataagaa gcatgaacgg catcccatct tcggtaatat
     4021 cgtcgacgag gtggcctatc acgagaaata cccaaccatc taccatcttc gcaaaaagct
     4081 ggtggactca accgacaagg cagacctccg gcttatctac ctggccctgg cccacatgat
     4141 caagttcaga ggccacttcc tgatcgaggg cgacctcaat cctgacaata gcgatgtgga
     4201 taaactgttc atccagctgg tgcagactta caaccagctc tttgaagaga accccatcaa
     4261 tgcaagcgga gtcgatgcca aggccattct gtcagcccgg ctgtcaaaga gccgcagact
     4321 tgagaatctt atcgctcagc tgccgggtga aaagaaaaat ggactgttcg ggaacctgat
     4381 tgctctttca cttgggctga ctcccaattt caagtctaat ttcgacctgg cagaggatgc
     4441 caagctgcaa ctgtccaagg acacctatga tgacgatctc gacaacctcc tggcccagat
     4501 cggtgaccaa tacgccgacc ttttccttgc tgctaagaat ctttctgacg ccatcctgct
     4561 gtctgacatt ctccgcgtga acactgaaat caccaaggcc cctctttcag cttcaatgat
     4621 taagcggtat gatgagcacc accaggacct gaccctgctt aaggcactcg tccggcagca
     4681 gcttccggag aagtacaagg aaatcttctt tgaccagtca aagaatggat acgccggcta
     4741 catcgacgga ggtgcctccc aagaggaatt ttataagttt atcaaaccta tccttgagaa
     4801 gatggacggc accgaagagc tcctcgtgaa actgaatcgg gaggatctgc tgcggaagca
     4861 gcgcactttc gacaatggga gcattcccca ccagatccat cttggggagc ttcacgccat
     4921 ccttcggcgc caagaggact tctacccctt tcttaaggac aacagggaga agattgagaa
     4981 aattctcact ttccgcatcc cctactacgt gggacccctc gccagaggaa atagccggtt
     5041 tgcttggatg accagaaagt cagaagaaac tatcactccc tggaacttcg aagaggtggt
     5101 ggacaaggga gccagcgctc agtcattcat cgaacggatg actaacttcg ataagaacct
     5161 ccccaatgag aaggtcctgc cgaaacattc cctgctctac gagtacttta ccgtgtacaa
     5221 cgagctgacc aaggtgaaat atgtcaccga agggatgagg aagcccgcat tcctgtcagg
     5281 cgaacaaaag aaggcaattg tggaccttct gttcaagacc aatagaaagg tgaccgtgaa
     5341 gcagctgaag gaggactatt tcaagaaaat tgaatgcttc gactctgtgg agattagcgg
     5401 ggtcgaagat cggttcaacg caagcctggg tacctaccat gatctgctta agatcatcaa
     5461 ggacaaggat tttctggaca atgaggagaa cgaggacatc cttgaggaca ttgtcctgac
     5521 tctcactctg ttcgaggacc gggaaatgat cgaggagagg cttaagacct acgcccatct
     5581 gttcgacgat aaagtgatga agcaacttaa acggagaaga tataccggat ggggacgcct
     5641 tagccgcaaa ctcatcaacg gaatccggga caaacagagc ggaaagacca ttcttgattt
     5701 ccttaaaagc gacggattcg ctaatcgcaa cttcatgcaa cttatccatg atgattccct
     5761 gacctttaag gaggacatcc agaaggccca agtgtctgga caaggtgact cactgcacga
     5821 gcatatcgca aatctggctg gttcacccgc tattaagaag ggtattctcc agaccgtgaa
     5881 agtcgtggac gagctggtca aggtgatggg tcgccataaa ccagagaaca ttgtcatcga
     5941 gatggccagg gaaaaccaga ctacccagaa gggacagaag aacagcaggg agcggatgaa
     6001 aagaattgag gaagggatta aggagctcgg gtcacagatc cttaaagagc acccggtgga
     6061 aaacacccag cttcagaatg agaagctcta tctgtactac cttcaaaatg gacgcgatat
     6121 gtatgtggac caagagcttg atatcaacag gctctcagac tacgacgtgg acgccatcgt
     6181 ccctcagagc ttcctcaaag acgactcaat tgacaataag gtgctgactc gctcagacaa
     6241 gaaccgggga aagtcagata acgtgccctc agaggaagtc gtgaaaaaga tgaagaacta
     6301 ttggcgccag cttctgaacg caaagctgat cactcagcgg aagttcgaca atctcactaa
     6361 ggctgagagg ggcggactga gcgaactgga caaagcagga ttcattaaac ggcaacttgt
     6421 ggagactcgg cagattacta aacatgtcgc ccaaatcctt gactcacgca tgaataccaa
     6481 gtacgacgaa aacgacaaac ttatccgcga ggtgaaggtg attaccctga agtccaagct
     6541 ggtcagcgat ttcagaaagg actttcaatt ctacaaagtg cgggagatca ataactatca
     6601 tcatgctcat gacgcatatc tgaatgccgt ggtgggaacc gccctgatca agaagtaccc
     6661 aaagctggaa agcgagttcg tgtacggaga ctacaaggtc tacgacgtgc gcaagatgat
     6721 tgccaaatct gagcaggaga tcggaaaggc caccgcaaag tacttcttct acagcaacat
     6781 catgaatttc ttcaagaccg aaatcaccct tgcaaacggt gagatccgga agaggccgct
     6841 catcgagact aatggggaga ctggcgaaat cgtgtgggac aagggcagag atttcgctac
     6901 cgtgcgcaaa gtgctttcta tgcctcaagt gaacatcgtg aagaaaaccg aggtgcaaac
     6961 cggaggcttt tctaaggaat caatcctccc caagcgcaac tccgacaagc tcattgcaag
     7021 gaagaaggat tgggacccta agaagtacgg cggattcgat tcaccaactg tggcttattc
     7081 tgtcctggtc gtggctaagg tggaaaaagg aaagtctaag aagctcaaga gcgtgaagga
     7141 actgctgggt atcaccatta tggagcgcag ctccttcgag aagaacccaa ttgactttct
     7201 cgaagccaaa ggttacaagg aagtcaagaa ggaccttatc atcaagctcc caaagtatag
     7261 cctgttcgaa ctggagaatg ggcggaagcg gatgctcgcc tccgctggcg aacttcagaa
     7321 gggtaatgag ctggctctcc cctccaagta cgtgaatttc ctctaccttg caagccatta
     7381 cgagaagctg aaggggagcc ccgaggacaa cgagcaaaag caactgtttg tggagcagca
     7441 taagcattat ctggacgaga tcattgagca gatttccgag ttttctaaac gcgtcattct
     7501 cgctgatgcc aacctcgata aagtccttag cgcatacaat aagcacagag acaaaccaat
     7561 tcgggagcag gctgagaata tcatccacct gttcaccctc accaatcttg gtgcccctgc
     7621 cgcattcaag tacttcgaca ccaccatcga ccggaaacgc tatacctcca ccaaagaagt
     7681 gctggacgcc accctcatcc accagagcat caccggactt tacgaaactc ggattgacct
     7741 ctcacagctc ggaggggatg gtggcggagg ctcgccaaaa aagaagagaa aggtagaccc
     7801 aaagaaaaaa cgaaaagtag atccgaaaaa gaagaggaag gtgggaggta gtggggctac
     7861 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctagcgagct
     7921 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     7981 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     8041 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     8101 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     8161 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     8221 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     8281 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     8341 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     8401 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     8461 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     8521 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     8581 ccctagcaaa ctggggcaca agcttaatta gtaaagcggc cgcactcctc aggtgcaggc
     8641 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     8701 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     8761 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     8821 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     8881 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     8941 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     9001 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     9061 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     9121 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     9181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9241 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9301 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     9361 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9421 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9481 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9541 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9601 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9661 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     9721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11641 taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
