LOCUS       sequence-418942-       12034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ISce1 restriction site is flanked by Tet operator repeats, 96 on 
            each side.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T
  TITLE     Spatial dynamics of chromosome translocations in living cells.
  JOURNAL   Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
  PUBMED    23929981
REFERENCE   2  (bases 1 to 12034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-08-9- 9"; volume: "341"; issue: 
            "6146"; pages: "660-4"
COMMENT     Sequence Label: pTetO-ISce1-TetO
FEATURES             Location/Qualifiers
     source          1..12034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(110..566)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        593..697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(698..766,767..1558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1729..2317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2605..2626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2641..2646,2647..2664,2665..2671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2679..2695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2703..2719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2740..2758
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(2868..2906)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(2933..2951,2952..2974,2975..2993,2994..3016,3017..3035,
                     3036..3058,3059..3077,3078..3100,3101..3119,3120..3142,
                     3143..3161,3162..3187,3188..3206)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2975..2993,2994..3016,3017..3035,3036..3058,3059..3077,
                     3078..3100,3101..3119,3120..3142,3143..3161,3162..3187,
                     3188..3206,3207..3229,3230..3248)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3017..3035,3036..3058,3059..3077,3078..3100,3101..3119,
                     3120..3142,3143..3161,3162..3187,3188..3206,3207..3229,
                     3230..3248,3249..3271,3272..3290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3059..3077,3078..3100,3101..3119,3120..3142,3143..3161,
                     3162..3187,3188..3206,3207..3229,3230..3248,3249..3271,
                     3272..3290,3291..3313,3314..3332)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3101..3119,3120..3142,3143..3161,3162..3187,3188..3206,
                     3207..3229,3230..3248,3249..3271,3272..3290,3291..3313,
                     3314..3332,3333..3355,3356..3374)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3143..3161,3162..3187,3188..3206,3207..3229,3230..3248,
                     3249..3271,3272..3290,3291..3313,3314..3332,3333..3355,
                     3356..3374,3375..3397,3398..3416)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3188..3206,3207..3229,3230..3248,3249..3271,3272..3290,
                     3291..3313,3314..3332,3333..3355,3356..3374,3375..3397,
                     3398..3416,3417..3442,3443..3461)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3230..3248,3249..3271,3272..3290,3291..3313,3314..3332,
                     3333..3355,3356..3374,3375..3397,3398..3416,3417..3442,
                     3443..3461,3462..3484,3485..3503)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3272..3290,3291..3313,3314..3332,3333..3355,3356..3374,
                     3375..3397,3398..3416,3417..3442,3443..3461,3462..3484,
                     3485..3503,3504..3526,3527..3545)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3314..3332,3333..3355,3356..3374,3375..3397,3398..3416,
                     3417..3442,3443..3461,3462..3484,3485..3503,3504..3526,
                     3527..3545,3546..3568,3569..3587)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3356..3374,3375..3397,3398..3416,3417..3442,3443..3461,
                     3462..3484,3485..3503,3504..3526,3527..3545,3546..3568,
                     3569..3587,3588..3610,3611..3629)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3398..3416,3417..3442,3443..3461,3462..3484,3485..3503,
                     3504..3526,3527..3545,3546..3568,3569..3587,3588..3610,
                     3611..3629,3630..3652,3653..3671)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3443..3461,3462..3484,3485..3503,3504..3526,3527..3545,
                     3546..3568,3569..3587,3588..3610,3611..3629,3630..3652,
                     3653..3671,3672..3697,3698..3716)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3485..3503,3504..3526,3527..3545,3546..3568,3569..3587,
                     3588..3610,3611..3629,3630..3652,3653..3671,3672..3697,
                     3698..3716,3717..3739,3740..3758)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3527..3545,3546..3568,3569..3587,3588..3610,3611..3629,
                     3630..3652,3653..3671,3672..3697,3698..3716,3717..3739,
                     3740..3758,3759..3781,3782..3800)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3569..3587,3588..3610,3611..3629,3630..3652,3653..3671,
                     3672..3697,3698..3716,3717..3739,3740..3758,3759..3781,
                     3782..3800,3801..3823,3824..3842)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3611..3629,3630..3652,3653..3671,3672..3697,3698..3716,
                     3717..3739,3740..3758,3759..3781,3782..3800,3801..3823,
                     3824..3842,3843..3865,3866..3884)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3653..3671,3672..3697,3698..3716,3717..3739,3740..3758,
                     3759..3781,3782..3800,3801..3823,3824..3842,3843..3865,
                     3866..3884,3885..3907,3908..3926)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3698..3716,3717..3739,3740..3758,3759..3781,3782..3800,
                     3801..3823,3824..3842,3843..3865,3866..3884,3885..3907,
                     3908..3926,3927..3952,3953..3971)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3740..3758,3759..3781,3782..3800,3801..3823,3824..3842,
                     3843..3865,3866..3884,3885..3907,3908..3926,3927..3952,
                     3953..3971,3972..3994,3995..4013)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3782..3800,3801..3823,3824..3842,3843..3865,3866..3884,
                     3885..3907,3908..3926,3927..3952,3953..3971,3972..3994,
                     3995..4013,4014..4036,4037..4055)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3824..3842,3843..3865,3866..3884,3885..3907,3908..3926,
                     3927..3952,3953..3971,3972..3994,3995..4013,4014..4036,
                     4037..4055,4056..4078,4079..4097)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3866..3884,3885..3907,3908..3926,3927..3952,3953..3971,
                     3972..3994,3995..4013,4014..4036,4037..4055,4056..4078,
                     4079..4097,4098..4120,4121..4139)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3908..3926,3927..3952,3953..3971,3972..3994,3995..4013,
                     4014..4036,4037..4055,4056..4078,4079..4097,4098..4120,
                     4121..4139,4140..4162,4163..4181)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3953..3971,3972..3994,3995..4013,4014..4036,4037..4055,
                     4056..4078,4079..4097,4098..4120,4121..4139,4140..4162,
                     4163..4181,4182..4207,4208..4226)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3995..4013,4014..4036,4037..4055,4056..4078,4079..4097,
                     4098..4120,4121..4139,4140..4162,4163..4181,4182..4207,
                     4208..4226,4227..4249,4250..4268)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4037..4055,4056..4078,4079..4097,4098..4120,4121..4139,
                     4140..4162,4163..4181,4182..4207,4208..4226,4227..4249,
                     4250..4268,4269..4291,4292..4310)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4079..4097,4098..4120,4121..4139,4140..4162,4163..4181,
                     4182..4207,4208..4226,4227..4249,4250..4268,4269..4291,
                     4292..4310,4311..4333,4334..4352)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4121..4139,4140..4162,4163..4181,4182..4207,4208..4226,
                     4227..4249,4250..4268,4269..4291,4292..4310,4311..4333,
                     4334..4352,4353..4375,4376..4394)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4163..4181,4182..4207,4208..4226,4227..4249,4250..4268,
                     4269..4291,4292..4310,4311..4333,4334..4352,4353..4375,
                     4376..4394,4395..4417,4418..4436)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4208..4226,4227..4249,4250..4268,4269..4291,4292..4310,
                     4311..4333,4334..4352,4353..4375,4376..4394,4395..4417,
                     4418..4436,4437..4459,4460..4478)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4250..4268,4269..4291,4292..4310,4311..4333,4334..4352,
                     4353..4375,4376..4394,4395..4417,4418..4436,4437..4459,
                     4460..4478,4479..4501,4502..4520)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4292..4310,4311..4333,4334..4352,4353..4375,4376..4394,
                     4395..4417,4418..4436,4437..4459,4460..4478,4479..4501,
                     4502..4520,4521..4546,4547..4565)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4334..4352,4353..4375,4376..4394,4395..4417,4418..4436,
                     4437..4459,4460..4478,4479..4501,4502..4520,4521..4546,
                     4547..4565,4566..4588,4589..4607)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4376..4394,4395..4417,4418..4436,4437..4459,4460..4478,
                     4479..4501,4502..4520,4521..4546,4547..4565,4566..4588,
                     4589..4607,4608..4630,4631..4649)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4418..4436,4437..4459,4460..4478,4479..4501,4502..4520,
                     4521..4546,4547..4565,4566..4588,4589..4607,4608..4630,
                     4631..4649,4650..4672,4673..4691)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4460..4478,4479..4501,4502..4520,4521..4546,4547..4565,
                     4566..4588,4589..4607,4608..4630,4631..4649,4650..4672,
                     4673..4691,4692..4714,4715..4733)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4502..4520,4521..4546,4547..4565,4566..4588,4589..4607,
                     4608..4630,4631..4649,4650..4672,4673..4691,4692..4714,
                     4715..4733,4734..4756,4757..4775)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4547..4565,4566..4588,4589..4607,4608..4630,4631..4649,
                     4650..4672,4673..4691,4692..4714,4715..4733,4734..4756,
                     4757..4775,4776..4801,4802..4820)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4589..4607,4608..4630,4631..4649,4650..4672,4673..4691,
                     4692..4714,4715..4733,4734..4756,4757..4775,4776..4801,
                     4802..4820,4821..4843,4844..4862)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4631..4649,4650..4672,4673..4691,4692..4714,4715..4733,
                     4734..4756,4757..4775,4776..4801,4802..4820,4821..4843,
                     4844..4862,4863..4885,4886..4904)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4673..4691,4692..4714,4715..4733,4734..4756,4757..4775,
                     4776..4801,4802..4820,4821..4843,4844..4862,4863..4885,
                     4886..4904,4905..4927,4928..4946)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4715..4733,4734..4756,4757..4775,4776..4801,4802..4820,
                     4821..4843,4844..4862,4863..4885,4886..4904,4905..4927,
                     4928..4946,4947..4969,4970..4988)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4757..4775,4776..4801,4802..4820,4821..4843,4844..4862,
                     4863..4885,4886..4904,4905..4927,4928..4946,4947..4969,
                     4970..4988,4989..5011,5012..5030)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4802..4820,4821..4843,4844..4862,4863..4885,4886..4904,
                     4905..4927,4928..4946,4947..4969,4970..4988,4989..5011,
                     5012..5030,5031..5056,5057..5075)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4844..4862,4863..4885,4886..4904,4905..4927,4928..4946,
                     4947..4969,4970..4988,4989..5011,5012..5030,5031..5056,
                     5057..5075,5076..5098,5099..5117)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4886..4904,4905..4927,4928..4946,4947..4969,4970..4988,
                     4989..5011,5012..5030,5031..5056,5057..5075,5076..5098,
                     5099..5117,5118..5140,5141..5158)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4928..4946,4947..4969,4970..4988,4989..5011,5012..5030,
                     5031..5056,5057..5075,5076..5098,5099..5117,5118..5140,
                     5141..5158,5159..5181,5182..5200)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4970..4988,4989..5011,5012..5030,5031..5056,5057..5075,
                     5076..5098,5099..5117,5118..5140,5141..5158,5159..5181,
                     5182..5200,5201..5223,5224..5242)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5012..5030,5031..5056,5057..5075,5076..5098,5099..5117,
                     5118..5140,5141..5158,5159..5181,5182..5200,5201..5223,
                     5224..5242,5243..5265,5266..5284)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5057..5075,5076..5098,5099..5117,5118..5140,5141..5158,
                     5159..5181,5182..5200,5201..5223,5224..5242,5243..5265,
                     5266..5284,5285..5310,5311..5329)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5099..5117,5118..5140,5141..5158,5159..5181,5182..5200,
                     5201..5223,5224..5242,5243..5265,5266..5284,5285..5310,
                     5311..5329,5330..5352,5353..5371)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5141..5158,5159..5181,5182..5200,5201..5223,5224..5242,
                     5243..5265,5266..5284,5285..5310,5311..5329,5330..5352,
                     5353..5371,5372..5394,5395..5413)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5182..5200,5201..5223,5224..5242,5243..5265,5266..5284,
                     5285..5310,5311..5329,5330..5352,5353..5371,5372..5394,
                     5395..5413,5414..5436,5437..5455)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5224..5242,5243..5265,5266..5284,5285..5310,5311..5329,
                     5330..5352,5353..5371,5372..5394,5395..5413,5414..5436,
                     5437..5455,5456..5478,5479..5497)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5266..5284,5285..5310,5311..5329,5330..5352,5353..5371,
                     5372..5394,5395..5413,5414..5436,5437..5455,5456..5478,
                     5479..5497,5498..5520,5521..5539)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5567..5585,5586..5608,5609..5627,5628..5650,5651..5669,
                     5670..5692,5693..5711,5712..5734,5735..5753,5754..5776,
                     5777..5795,5796..5821,5822..5840)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5609..5627,5628..5650,5651..5669,5670..5692,5693..5711,
                     5712..5734,5735..5753,5754..5776,5777..5795,5796..5821,
                     5822..5840,5841..5863,5864..5882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5651..5669,5670..5692,5693..5711,5712..5734,5735..5753,
                     5754..5776,5777..5795,5796..5821,5822..5840,5841..5863,
                     5864..5882,5883..5905,5906..5924)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5693..5711,5712..5734,5735..5753,5754..5776,5777..5795,
                     5796..5821,5822..5840,5841..5863,5864..5882,5883..5905,
                     5906..5924,5925..5947,5948..5966)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5735..5753,5754..5776,5777..5795,5796..5821,5822..5840,
                     5841..5863,5864..5882,5883..5905,5906..5924,5925..5947,
                     5948..5966,5967..5989,5990..6008)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5777..5795,5796..5821,5822..5840,5841..5863,5864..5882,
                     5883..5905,5906..5924,5925..5947,5948..5966,5967..5989,
                     5990..6008,6009..6031,6032..6050)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5822..5840,5841..5863,5864..5882,5883..5905,5906..5924,
                     5925..5947,5948..5966,5967..5989,5990..6008,6009..6031,
                     6032..6050,6051..6076,6077..6095)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5864..5882,5883..5905,5906..5924,5925..5947,5948..5966,
                     5967..5989,5990..6008,6009..6031,6032..6050,6051..6076,
                     6077..6095,6096..6118,6119..6137)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5906..5924,5925..5947,5948..5966,5967..5989,5990..6008,
                     6009..6031,6032..6050,6051..6076,6077..6095,6096..6118,
                     6119..6137,6138..6160,6161..6179)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5948..5966,5967..5989,5990..6008,6009..6031,6032..6050,
                     6051..6076,6077..6095,6096..6118,6119..6137,6138..6160,
                     6161..6179,6180..6202,6203..6221)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5990..6008,6009..6031,6032..6050,6051..6076,6077..6095,
                     6096..6118,6119..6137,6138..6160,6161..6179,6180..6202,
                     6203..6221,6222..6244,6245..6263)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6032..6050,6051..6076,6077..6095,6096..6118,6119..6137,
                     6138..6160,6161..6179,6180..6202,6203..6221,6222..6244,
                     6245..6263,6264..6286,6287..6305)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6077..6095,6096..6118,6119..6137,6138..6160,6161..6179,
                     6180..6202,6203..6221,6222..6244,6245..6263,6264..6286,
                     6287..6305,6306..6331,6332..6350)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6119..6137,6138..6160,6161..6179,6180..6202,6203..6221,
                     6222..6244,6245..6263,6264..6286,6287..6305,6306..6331,
                     6332..6350,6351..6373,6374..6392)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6161..6179,6180..6202,6203..6221,6222..6244,6245..6263,
                     6264..6286,6287..6305,6306..6331,6332..6350,6351..6373,
                     6374..6392,6393..6415,6416..6434)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6203..6221,6222..6244,6245..6263,6264..6286,6287..6305,
                     6306..6331,6332..6350,6351..6373,6374..6392,6393..6415,
                     6416..6434,6435..6457,6458..6476)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6245..6263,6264..6286,6287..6305,6306..6331,6332..6350,
                     6351..6373,6374..6392,6393..6415,6416..6434,6435..6457,
                     6458..6476,6477..6499,6500..6518)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6287..6305,6306..6331,6332..6350,6351..6373,6374..6392,
                     6393..6415,6416..6434,6435..6457,6458..6476,6477..6499,
                     6500..6518,6519..6541,6542..6560)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6332..6350,6351..6373,6374..6392,6393..6415,6416..6434,
                     6435..6457,6458..6476,6477..6499,6500..6518,6519..6541,
                     6542..6560,6561..6586,6587..6605)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6374..6392,6393..6415,6416..6434,6435..6457,6458..6476,
                     6477..6499,6500..6518,6519..6541,6542..6560,6561..6586,
                     6587..6605,6606..6628,6629..6647)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6416..6434,6435..6457,6458..6476,6477..6499,6500..6518,
                     6519..6541,6542..6560,6561..6586,6587..6605,6606..6628,
                     6629..6647,6648..6670,6671..6689)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6458..6476,6477..6499,6500..6518,6519..6541,6542..6560,
                     6561..6586,6587..6605,6606..6628,6629..6647,6648..6670,
                     6671..6689,6690..6712,6713..6731)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6500..6518,6519..6541,6542..6560,6561..6586,6587..6605,
                     6606..6628,6629..6647,6648..6670,6671..6689,6690..6712,
                     6713..6731,6732..6754,6755..6773)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6542..6560,6561..6586,6587..6605,6606..6628,6629..6647,
                     6648..6670,6671..6689,6690..6712,6713..6731,6732..6754,
                     6755..6773,6774..6796,6797..6815)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6587..6605,6606..6628,6629..6647,6648..6670,6671..6689,
                     6690..6712,6713..6731,6732..6754,6755..6773,6774..6796,
                     6797..6815,6816..6841,6842..6860)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6629..6647,6648..6670,6671..6689,6690..6712,6713..6731,
                     6732..6754,6755..6773,6774..6796,6797..6815,6816..6841,
                     6842..6860,6861..6883,6884..6902)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6671..6689,6690..6712,6713..6731,6732..6754,6755..6773,
                     6774..6796,6797..6815,6816..6841,6842..6860,6861..6883,
                     6884..6902,6903..6925,6926..6944)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6713..6731,6732..6754,6755..6773,6774..6796,6797..6815,
                     6816..6841,6842..6860,6861..6883,6884..6902,6903..6925,
                     6926..6944,6945..6967,6968..6986)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6755..6773,6774..6796,6797..6815,6816..6841,6842..6860,
                     6861..6883,6884..6902,6903..6925,6926..6944,6945..6967,
                     6968..6986,6987..7009,7010..7028)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6797..6815,6816..6841,6842..6860,6861..6883,6884..6902,
                     6903..6925,6926..6944,6945..6967,6968..6986,6987..7009,
                     7010..7028,7029..7051,7052..7070)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6842..6860,6861..6883,6884..6902,6903..6925,6926..6944,
                     6945..6967,6968..6986,6987..7009,7010..7028,7029..7051,
                     7052..7070,7071..7096,7097..7115)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6884..6902,6903..6925,6926..6944,6945..6967,6968..6986,
                     6987..7009,7010..7028,7029..7051,7052..7070,7071..7096,
                     7097..7115,7116..7138,7139..7157)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6926..6944,6945..6967,6968..6986,6987..7009,7010..7028,
                     7029..7051,7052..7070,7071..7096,7097..7115,7116..7138,
                     7139..7157,7158..7180,7181..7199)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6968..6986,6987..7009,7010..7028,7029..7051,7052..7070,
                     7071..7096,7097..7115,7116..7138,7139..7157,7158..7180,
                     7181..7199,7200..7222,7223..7241)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7010..7028,7029..7051,7052..7070,7071..7096,7097..7115,
                     7116..7138,7139..7157,7158..7180,7181..7199,7200..7222,
                     7223..7241,7242..7264,7265..7283)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7052..7070,7071..7096,7097..7115,7116..7138,7139..7157,
                     7158..7180,7181..7199,7200..7222,7223..7241,7242..7264,
                     7265..7283,7284..7306,7307..7325)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7097..7115,7116..7138,7139..7157,7158..7180,7181..7199,
                     7200..7222,7223..7241,7242..7264,7265..7283,7284..7306,
                     7307..7325,7326..7351,7352..7370)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7139..7157,7158..7180,7181..7199,7200..7222,7223..7241,
                     7242..7264,7265..7283,7284..7306,7307..7325,7326..7351,
                     7352..7370,7371..7393,7394..7412)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7181..7199,7200..7222,7223..7241,7242..7264,7265..7283,
                     7284..7306,7307..7325,7326..7351,7352..7370,7371..7393,
                     7394..7412,7413..7435,7436..7454)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7223..7241,7242..7264,7265..7283,7284..7306,7307..7325,
                     7326..7351,7352..7370,7371..7393,7394..7412,7413..7435,
                     7436..7454,7455..7477,7478..7496)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7265..7283,7284..7306,7307..7325,7326..7351,7352..7370,
                     7371..7393,7394..7412,7413..7435,7436..7454,7455..7477,
                     7478..7496,7497..7519,7520..7538)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7307..7325,7326..7351,7352..7370,7371..7393,7394..7412,
                     7413..7435,7436..7454,7455..7477,7478..7496,7497..7519,
                     7520..7538,7539..7561,7562..7579)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7352..7370,7371..7393,7394..7412,7413..7435,7436..7454,
                     7455..7477,7478..7496,7497..7519,7520..7538,7539..7561,
                     7562..7580,7581..7606,7607..7625)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7394..7412,7413..7435,7436..7454,7455..7477,7478..7496,
                     7497..7519,7520..7538,7539..7561,7562..7580,7581..7606,
                     7607..7625,7626..7648,7649..7667)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7436..7454,7455..7477,7478..7496,7497..7519,7520..7538,
                     7539..7561,7562..7580,7581..7606,7607..7625,7626..7648,
                     7649..7667,7668..7690,7691..7709)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7478..7496,7497..7519,7520..7538,7539..7561,7562..7580,
                     7581..7606,7607..7625,7626..7648,7649..7667,7668..7690,
                     7691..7709,7710..7732,7733..7751)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7520..7538,7539..7561,7562..7580,7581..7606,7607..7625,
                     7626..7648,7649..7667,7668..7690,7691..7709,7710..7732,
                     7733..7751,7752..7774,7775..7793)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7562..7580,7581..7606,7607..7625,7626..7648,7649..7667,
                     7668..7690,7691..7709,7710..7732,7733..7751,7752..7774,
                     7775..7793,7794..7816,7817..7835)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7607..7625,7626..7648,7649..7667,7668..7690,7691..7709,
                     7710..7732,7733..7751,7752..7774,7775..7793,7794..7816,
                     7817..7835,7836..7861,7862..7880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7649..7667,7668..7690,7691..7709,7710..7732,7733..7751,
                     7752..7774,7775..7793,7794..7816,7817..7835,7836..7861,
                     7862..7880,7881..7903,7904..7922)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7691..7709,7710..7732,7733..7751,7752..7774,7775..7793,
                     7794..7816,7817..7835,7836..7861,7862..7880,7881..7903,
                     7904..7922,7923..7945,7946..7964)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7733..7751,7752..7774,7775..7793,7794..7816,7817..7835,
                     7836..7861,7862..7880,7881..7903,7904..7922,7923..7945,
                     7946..7964,7965..7987,7988..8006)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7775..7793,7794..7816,7817..7835,7836..7861,7862..7880,
                     7881..7903,7904..7922,7923..7945,7946..7964,7965..7987,
                     7988..8006,8007..8029,8030..8048)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7817..7835,7836..7861,7862..7880,7881..7903,7904..7922,
                     7923..7945,7946..7964,7965..7987,7988..8006,8007..8029,
                     8030..8048,8049..8071,8072..8090)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7862..7880,7881..7903,7904..7922,7923..7945,7946..7964,
                     7965..7987,7988..8006,8007..8029,8030..8048,8049..8071,
                     8072..8090,8091..8116,8117..8135)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7904..7922,7923..7945,7946..7964,7965..7987,7988..8006,
                     8007..8029,8030..8048,8049..8071,8072..8090,8091..8116,
                     8117..8135,8136..8158,8159..8177)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7946..7964,7965..7987,7988..8006,8007..8029,8030..8048,
                     8049..8071,8072..8090,8091..8116,8117..8135,8136..8158,
                     8159..8177,8178..8200,8201..8219)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7988..8006,8007..8029,8030..8048,8049..8071,8072..8090,
                     8091..8116,8117..8135,8136..8158,8159..8177,8178..8200,
                     8201..8219,8220..8242,8243..8261)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8030..8048,8049..8071,8072..8090,8091..8116,8117..8135,
                     8136..8158,8159..8177,8178..8200,8201..8219,8220..8242,
                     8243..8261,8262..8284,8285..8303)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8072..8090,8091..8116,8117..8135,8136..8158,8159..8177,
                     8178..8200,8201..8219,8220..8242,8243..8261,8262..8284,
                     8285..8303,8304..8326,8327..8345)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8117..8135,8136..8158,8159..8177,8178..8200,8201..8219,
                     8220..8242,8243..8261,8262..8284,8285..8303,8304..8326,
                     8327..8345,8346..8371,8372..8390)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8159..8177,8178..8200,8201..8219,8220..8242,8243..8261,
                     8262..8284,8285..8303,8304..8326,8327..8345,8346..8371,
                     8372..8390,8391..8413,8414..8432)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8201..8219,8220..8242,8243..8261,8262..8284,8285..8303,
                     8304..8326,8327..8345,8346..8371,8372..8390,8391..8413,
                     8414..8432,8433..8455,8456..8474)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8243..8261,8262..8284,8285..8303,8304..8326,8327..8345,
                     8346..8371,8372..8390,8391..8413,8414..8432,8433..8455,
                     8456..8474,8475..8497,8498..8516)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8285..8303,8304..8326,8327..8345,8346..8371,8372..8390,
                     8391..8413,8414..8432,8433..8455,8456..8474,8475..8497,
                     8498..8516,8517..8539,8540..8558)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8327..8345,8346..8371,8372..8390,8391..8413,8414..8432,
                     8433..8455,8456..8474,8475..8497,8498..8516,8517..8539,
                     8540..8558,8559..8581,8582..8600)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8372..8390,8391..8413,8414..8432,8433..8455,8456..8474,
                     8475..8497,8498..8516,8517..8539,8540..8558,8559..8581,
                     8582..8600,8601..8626,8627..8645)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8414..8432,8433..8455,8456..8474,8475..8497,8498..8516,
                     8517..8539,8540..8558,8559..8581,8582..8600,8601..8626,
                     8627..8645,8646..8668,8669..8687)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8456..8474,8475..8497,8498..8516,8517..8539,8540..8558,
                     8559..8581,8582..8600,8601..8626,8627..8645,8646..8668,
                     8669..8687,8688..8710,8711..8729)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8498..8516,8517..8539,8540..8558,8559..8581,8582..8600,
                     8601..8626,8627..8645,8646..8668,8669..8687,8688..8710,
                     8711..8729,8730..8752,8753..8771)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8540..8558,8559..8581,8582..8600,8601..8626,8627..8645,
                     8646..8668,8669..8687,8688..8710,8711..8729,8730..8752,
                     8753..8771,8772..8794,8795..8813)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8582..8600,8601..8626,8627..8645,8646..8668,8669..8687,
                     8688..8710,8711..8729,8730..8752,8753..8771,8772..8794,
                     8795..8813,8814..8836,8837..8855)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8627..8645,8646..8668,8669..8687,8688..8710,8711..8729,
                     8730..8752,8753..8771,8772..8794,8795..8813,8814..8836,
                     8837..8855,8856..8879,8880..8898)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8669..8687,8688..8710,8711..8729,8730..8752,8753..8771,
                     8772..8794,8795..8813,8814..8836,8837..8855,8856..8879,
                     8880..8898,8899..8921,8922..8940)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8711..8729,8730..8752,8753..8771,8772..8794,8795..8813,
                     8814..8836,8837..8855,8856..8879,8880..8898,8899..8921,
                     8922..8940,8941..8963,8964..8982)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(9019..9038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9020..9038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9048..9065)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9048..9064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9057..9079)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9205..9661)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9688..9792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9793..9861,9862..10653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10824..11412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11700..11721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11736..11741,11742..11759,11760..11766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11774..11790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11798..11814
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11835..11853
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(11963..12001)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
       61 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
      121 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt tttttaacca
      181 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      241 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      301 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      361 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      421 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      481 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      541 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
      601 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      661 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      721 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      781 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      841 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      901 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      961 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1021 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1081 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1141 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1201 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1261 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1321 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1381 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1441 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1501 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1561 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1621 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1681 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1861 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2341 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2401 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2461 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2581 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2641 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2701 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     2761 acaaaagctg gagctccacc gcggaggctg gatcggtccc ggtgtcttct atggaggtca
     2821 aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata
     2881 taggcctccc accgtacacg cctacctcga cccggtcgac gactttcact tttctctatc
     2941 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     3001 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     3061 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     3121 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgacgactt
     3181 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     3241 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     3301 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     3361 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     3421 taaactcgac gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
     3481 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
     3541 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     3601 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     3661 actgataggg agtggtaaac tcgacgactt tcacttttct ctatcactga tagggagtgg
     3721 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     3781 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     3841 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     3901 tcacttttct ctatcactga tagggagtgg taaactcgtc gactttcact tttctctatc
     3961 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     4021 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     4081 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     4141 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgacgactt
     4201 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     4261 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     4321 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     4381 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     4441 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     4501 ttctctatca ctgataggga gtggtaaact cgacgacttt cacttttctc tatcactgat
     4561 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     4621 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     4681 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     4741 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgacg actttcactt
     4801 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     4861 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     4921 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     4981 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     5041 cgacgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     5101 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tatagggagt
     5161 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
     5221 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
     5281 gggagtggta aactcgacga ctttcacttt tctctatcac tgatagggag tggtaaactc
     5341 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
     5401 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
     5461 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
     5521 tctctatcac tgatagggag tggtaaactc gaccgacttt cacttttctc tatcactgat
     5581 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     5641 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     5701 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     5761 actcgacttt cacttttctc tatcactgat agggagtggt aatctcgacg actttcactt
     5821 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     5881 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     5941 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     6001 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     6061 cgacgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     6121 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     6181 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
     6241 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
     6301 agggagtggt aaactcgacg actttcactt ttctctatca ctgataggga gtggtaaact
     6361 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     6421 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     6481 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     6541 ttctctatca ctgataggga gtggtaatct cgacgacttt cacttttctc tatcactgat
     6601 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     6661 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     6721 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     6781 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgacg actttcactt
     6841 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     6901 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     6961 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     7021 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     7081 cgacgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     7141 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     7201 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
     7261 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
     7321 agggagtggt aaactcgacg actttcactt ttctctatca ctgataggga gtggtaaact
     7381 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     7441 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     7501 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     7561 ttctctatca ctgatagggt gtggtaaact cgacgacttt cacttttctc tatcactgat
     7621 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     7681 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     7741 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     7801 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgacg actttcactt
     7861 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     7921 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     7981 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     8041 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     8101 cgacgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     8161 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     8221 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
     8281 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
     8341 agggagtggt aaactcgacg actttcactt ttctctatca ctgataggga gtggtaaact
     8401 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     8461 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     8521 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     8581 ttctctatca ctgataggga gtggtaaact cgacgacttt cacttttctc tatcactgat
     8641 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     8701 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     8761 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     8821 actcgacttt cacttttctc tatcactgat agggagtggt aaactccgac tttcactttt
     8881 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     8941 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgaggggg
     9001 ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     9061 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9121 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9181 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     9241 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
     9301 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     9361 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     9421 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     9481 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     9541 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     9601 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     9661 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9721 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9781 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9841 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9901 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9961 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10021 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10081 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10141 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10201 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10261 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10321 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10381 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10441 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10501 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10561 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10621 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10681 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10741 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10801 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10861 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10921 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10981 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11041 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11101 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11161 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11221 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11281 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11341 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11401 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11461 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11521 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11581 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    11701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    11761 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    11821 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcgga
    11881 ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
    11941 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac
    12001 ctcgacccgg tcgacgactt tcacttttct ctat
//
