LOCUS       sequence-418955-        8138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent protein splicing reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Orengo JP, Bundman D, Cooper TA
  TITLE     A bichromatic fluorescent reporter for cell-based screens of 
            alternative splicing.
  JOURNAL   Nucleic Acids Res. 2006;34(22):e148. Epub 2006 Nov 16.
  PUBMED    17142220
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2006"; volume: "34(22)"; pages: "e148. Epub 2006
            Nov 16"
COMMENT     Sequence Label: RG6
FEATURES             Location/Qualifiers
     source          1..8138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     200..219
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(340..669)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      354..489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(408..427)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     654..674
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(683..1111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(793..814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1005..1024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1157..1381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1370..1387
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(1413..2126,2127..2129))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1416..2126,2127..2129))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1458..1479)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1807..1826
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2066..2087
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(join(2138..2812,2813..2815))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2200..2223)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2398..2417)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2615..2635
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     CDS             complement(4138..4158)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4171..4194)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4234..4253)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4235..4253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4298..4501)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4327..4347)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4502..4881
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5053..5072
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5147..5251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5252..5320,5321..6112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5470..5489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6283..6871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6772..6791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7025..7042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7159..7180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7195..7200,7201..7218,7219..7225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7233..7249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7238..7260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7257..7273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7257..7273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7322..7443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7333..7352)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7387..7406
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(7617..8138,1..273))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7728..7747)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
       61 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
      121 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
      181 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
      241 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
      301 atcagatccg aaaatggata tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc
      361 aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc
      421 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
      481 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
      541 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
      601 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
      661 ctgacacaca ttccacagaa ttaattcgcg ttaaattttt gttaaatcag ctcatttttt
      721 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      781 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
      841 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
      901 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
      961 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1021 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1081 gccgcgctta atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt
     1141 ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc
     1201 caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc
     1261 agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc
     1321 agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca
     1381 gtcgaggctg atcagcgggt ttaaacgggc ccttacttgt acagctcgtc catgccgaga
     1441 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     1501 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     1561 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     1621 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
     1681 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     1741 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     1801 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     1861 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
     1921 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     1981 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     2041 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     2101 ccggtgaaca gctcctcgcc cttgctcaca ccgcggtcta caggaacagg tggtggcggc
     2161 cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     2221 gcttggagtc cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg
     2281 acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc
     2341 ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct
     2401 tcttctgcat tacggggccg tcggagggga agttcacgcc gatgaacttc accttgtaga
     2461 tgaagcagcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     2521 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg tagtcgggga
     2581 tgtcggcggg gtgcttcacg tacaccttgg agccgtactg gaactggggg gacaggatgt
     2641 cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttcacggtg ttgtggccct
     2701 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggtgccct
     2761 ccatgcgcac cttgaagcgc atgaactcct tgatgacgtt cttggaggag cgcacaccgg
     2821 tctcctcttc ctcctcgtcc tcctgatcct cctgacctgt gcaataagga cagggtcagt
     2881 aggacggccg cagctcctgc tggctcccta cagaccccca gaatgggaca accgtggcac
     2941 tgagtccagg agacccgaag gagagggagc tatgggagaa agaagcagga agtaaagggc
     3001 tctgttcagg cccttacctg aaggtaaggc atggatgagg gggaataaat ccaaggactc
     3061 ggagccccct cctggttttg tcccttgttc cctccccagc tgtgcccccc tcgaggggga
     3121 ccccagagtg ggggagatcg acttctgctg ctcccctcca tgcagcagag agcaaaactg
     3181 tatggcataa atctcctgca tgacactgct ggagcatttt tccactggag ggagcagtac
     3241 ccccacgaga gtcctctgtc agagcccgca ggtcctgcag gagacgtttg gtcccacagc
     3301 aagagaggaa gaaaggagaa agaagacagc aacccagggg tgtgttggga tggaagacag
     3361 aaagcacaga actgagagca ggttggcaca gcaggatggg gagagcgcaa aaagcagcag
     3421 agaagtgaaa gaatgaaagg aaagcgtttc ccaagcaggg aaaggcacag gacacaccga
     3481 cccgcgattt attcacagca gaggtggaga gatggaacaa agaaaggtgc agtaactacc
     3541 tgaattcttc cggatatcgc ctggatcctg agccagggag gagggaaggg aagaagcgca
     3601 gaggttagcc aggtgcctta aagctgtggg gcagagctac ggtgtgcacc aggtgaggac
     3661 gccgaccctt tcagctctca ggatgtgtgg ctgcatggca gcactgaagc agcaggagcc
     3721 ccagcagacc ccggttccca ttgcccaccc cgtacagaca gccccactgc tgcagcatgg
     3781 ggtttggtcc tgcactgtgc tgatggagcc gggggttctg cttgagcatt gttaattgca
     3841 ttcagcatcg atgctgaagt tccaatgcct gttgaattat tgtgcacaca tccagcattg
     3901 ccatctccct ctttgggccg agggatctcc ctgggaaaag agatcctgag aaaacacggg
     3961 gtggctttgg tcctaccggc cggtggttga ggtggaggca ttcagaacat ggtcccaatg
     4021 gccttttctg ctcagtctgc ccatggcaag gaaatcacca cccccccccc tcaagcaaaa
     4081 ccctgaacca aagcagcagg aggatcacct acctctagat cctggtactg ggtcctccac
     4141 tttgcgtttt ttttttgggg caggtacccc cttgtcatcg tcatccttgt aatccatgga
     4201 agcttaagtt taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga
     4261 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
     4321 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     4381 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     4441 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     4501 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     4561 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     4621 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     4681 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     4741 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     4801 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     4861 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
     4921 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     4981 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     5041 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag
     5101 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5161 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5221 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5281 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5341 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5401 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5461 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5521 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5581 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5641 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5701 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5761 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5821 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5881 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5941 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6001 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6061 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6121 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6181 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6421 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7021 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7081 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7141 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7201 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7261 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg
     7321 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     7381 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     7441 gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     7501 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     7561 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     7621 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     7681 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     7741 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     7801 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     7861 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     7921 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     7981 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     8041 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     8101 gacaggagat cctgccccgg cacttcgccc aatagcag
//
