LOCUS       sequence-418960-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative selection marker for use with the Mos-1 mediated system of 
            creating targeted transgene insertions and targeted deletions in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pMA122 - peel-1 negative selection
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     190..209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     416..438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     435..451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     435..451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    471..491
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    991..1015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1544..1568)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1573..1896
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(1900..1920)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1928..1945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1928..1944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1937..1959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2272..2294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2332..2350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2418..2522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2523..2591,2592..3383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2741..2760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3554..3853,1..289)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
       61 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      301 cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc
      361 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg
      421 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat caactttgta
      481 tagaaaagtt gctacaggtc gactctagag gatcaccaaa aacggaacgt tgagctggac
      541 ggaaatagtg gtaaagtgac atgattatag tttgaagatt tctaatttca caattagagc
      601 aaatgttgtt cggtatttat tttcaacggt atttatacta ttttccacct ttttctagaa
      661 cattcgagct gcttgttgca aaaggagggc gactcacatt cggtacatgg aaaagtagtg
      721 tacacaataa agagacccag atacattttc cgtctgcgtc tctttgcacc caccgggagt
      781 attttcaaac gaatgcatct aggaccttct agaacattct gtaaggctgc agaatgcggg
      841 tatataagga aagcgggctc agaggaagcc aacacgcttt gttctagtgc atctaaaaaa
      901 cttcgaaaat cctcatcgga tcctatcgat tcgcggccgc tgtacacccg ggtgccctat
      961 agtgagtcgt attggtaccg gtagaaaaaa tcaagtttgt acaaaaaagc aggcttcatg
     1021 cgctttgatt tccaaaactt aaaattttcg atgattttca tatttttgtg gaacatcata
     1081 gtcggatttt tacttgctct tgtaaagatt ttcaaaatct acatgtatct tgatctgcct
     1141 gaaaatgtat gggtaaatcg gtttgcgcat gttattgctc ttattggatc aattgcttca
     1201 ttgtggttaa ttttttttag cccatttgaa attaagcaag gaagattttc tgtctcgtca
     1261 aaaactatcc tgcttcttgt ttgcaagggt ttgattggct tgatatttct tgcacagatt
     1321 ttcgcccatg tcctttacat tgacccatgc cagaatatgc tcttatcatt gaactctgca
     1381 ctgttctcct tgctgctgaa ttattatgag tctcgtggtg cgatccatcc gttgatggaa
     1441 ttagttcaaa cagctggcct gagaccaact ttgtgcgctg ctgtacgatt taattgtctc
     1501 aactcaactg ctcatattga tccaagtgtt gaaaatccat gagacccagc tttcttgtac
     1561 aaagtgggat aaatgcaaaa tcctttcaag cattcccttc ttctctatca ctcttctttc
     1621 tttttgtcaa aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac
     1681 tttttatagt cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc
     1741 tgtctgatgt actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa
     1801 ggttgagaaa aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa
     1861 aagaggaaac tttattgtgc cgccaagaaa aaagtctcac aactttatta tacatagttg
     1921 ataattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1981 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2041 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     2101 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtgcaatct
     2161 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2221 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2281 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     2341 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     2401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2761 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3061 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3241 ctcgcggtat cattgcagca ctggggtcag atggtaagcc ctcccgtatc gtagttatct
     3301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3361 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3421 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3481 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3541 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3601 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3661 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3721 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3781 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3841 agttaccgga taa
//
