LOCUS       sequence-418961-        7458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for T7 RNA polymerase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7458)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-T7pol
FEATURES             Location/Qualifiers
     source          1..7458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          716..1732
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1656..1678
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1740..1759
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4533..4552)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4598..4653
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4652..4671)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5014..5030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5014..5030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5027..5049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5038..5054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5062..5068,5069..5086,5087..5092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5107..5128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5187..5382
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5233..5368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5388..5522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5425..5444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5479..5498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5590..5607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5761..6349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5841..5860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6520..7311,7312..7380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7143..7162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7381..7458,1..27))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac
       61 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      121 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      421 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc tcccccctcc
      481 ccccctcccc ccgccccgtg gtgtggtttt ttttgttttt ggtttggttg gggggggggg
      541 gggggggggg gggggggggc gcgcgccggg gggggggggg gggggcgagg ggcggggcgg
      601 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      661 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      721 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
      781 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      841 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
      901 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
      961 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     1021 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     1081 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1141 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     1201 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     1261 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1321 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     1381 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1441 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1501 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     1561 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     1621 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1681 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1741 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcgccg ccaccatgaa
     1801 cacgattaac atcgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa
     1861 cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga
     1921 gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg
     1981 tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat
     2041 tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc
     2101 cttccagttc ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac
     2161 tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg
     2221 tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt
     2281 caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt
     2341 tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc
     2401 ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattga
     2461 gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga
     2521 gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc
     2581 tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac
     2641 tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa
     2701 gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat
     2761 tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac
     2821 caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat
     2881 gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc
     2941 tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg agttcatgct
     3001 tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg
     3061 gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg
     3121 actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat
     3181 ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat
     3241 tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg
     3301 ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca
     3361 gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgg
     3421 catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttaacttgct
     3481 tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct
     3541 acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac
     3601 tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc
     3661 ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg acgctggctt acgggtccaa
     3721 agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta ttgattccgg
     3781 caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta agctgatttg
     3841 ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc
     3901 tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg
     3961 ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc
     4021 tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc ctaccattaa
     4081 caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg ctcctaactt
     4141 tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta
     4201 cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc
     4261 gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact
     4321 ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc
     4381 agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt
     4441 cgcgtaagcg gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     4501 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     4561 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     4621 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     4681 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     4741 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     4801 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     4861 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     4921 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     4981 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5161 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
     5221 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5281 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5341 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
     5401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5521 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5581 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5641 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5701 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6361 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7441 catatttgaa tgtattta
//
