LOCUS       sequence-419009-        9375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TopFlash reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Conrad JV, Meyer S, Ramesh PS, Neira JA, Rusteika M, Mamott D, 
            Duffin B, Bautista M, Zhang J, Hiles E, Higgins EM, Steill J, 
            Freeman J, Ni Z, Liu S, Ungrin M, Rancourt D, Clegg DO, Stewart R, 
            Thomson JA, Chu LF
  TITLE     Efficient derivation of transgene-free porcine induced pluripotent 
            stem cells enables in vitro modeling of species-specific 
            developmental timing.
  JOURNAL   Stem Cell Reports. 2023 Dec 12;18(12):2328-2343. doi: 
            10.1016/j.stemcr.2023.10.009. Epub 2023 Nov 9.
  PUBMED    37949072
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2023.10.009"; journalName: "Stem Cell Reports"; 
            date: "2023-12-12- 12"; volume: "18"; issue: "12"; pages: 
            "2328-2343"
COMMENT     Sequence Label: pB-TopFlash-NanoLucP
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   418..480
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1570..1601
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1663..1672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1669..2181
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             2185..2304
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2356..2477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2491..3653
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2722..3644
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3601..3621
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3709..3718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3715..3717,3718..3720,3721..4434)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3760..3781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4021..4040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4368..4389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          4502..4731
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4714..4733
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4803..5377,5378..5380,5381..5389)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4970..4987)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5197..5216
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5409..6008
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5409..5428)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5905..5925
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6138..6272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6743..6776)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     repeat_region   7094..7128
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7154..7170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7154..7170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7167..7189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7178..7194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7202..7208,7209..7226,7227..7232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7247..7268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7385..7402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7556..8144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7636..7655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8315..9106,9107..9175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8938..8957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9176..9280)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9348..9366
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caagctttat tatttaaccc
      421 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
      481 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
      541 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
      601 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
      661 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtaatcgat
      721 aataagtaat tagcttaacc tatacataat agaaacaaat tatacttatt agtcagtcag
      781 aaacaacttt ggcacatatc aatattatgc tctcgttaat taactcgagg gatccggcgc
      841 gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt
      901 ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc
      961 cgcgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     1021 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     1081 ggctttggga gggggctgtc cccgtgagct cccaggcgcg cctgtcattc taaatctctc
     1141 tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     1201 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     1261 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     1321 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     1381 gtccccgtga gctcccaggc gcgccttaag gtaccgagct cttacgcgag atcaaagggg
     1441 gtaagatcaa agggggtaag atcaaagggg cgcgagatca aagggggtaa gatcaaaggg
     1501 ggtaagatca aagggggtaa gatcaaaggg gcgcgcccgc gtgctagccc gggctcgaga
     1561 tctagactct agagggtata taatggaagc tcgaattcca gcttggcatt ccggtactgt
     1621 tggtaaaaag cttacgcgtg gcaatccggt actgttggta aagccaccat ggtcttcaca
     1681 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     1741 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     1801 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     1861 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     1921 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     1981 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     2041 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     2101 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     2161 ctgtgcgaac gcattctggc gaattctcac ggctttccgc ctgaggttga agagcaagcc
     2221 gccggtacat tgcctatgtc ctgcgcacaa gaaagcggta tggaccggca cccagccgct
     2281 tgtgcttcag ctcgcatcaa cgtctaaggc cgcgactcta gagtcggggc ggccggccgc
     2341 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2401 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     2461 gctgcaataa acaagttgct agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc
     2521 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     2581 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2641 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2701 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     2761 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt
     2821 gatcccgagc tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt
     2881 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     2941 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     3001 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt
     3061 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     3121 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     3181 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     3241 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg
     3301 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     3361 aaggggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     3421 ccaggcacct cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg
     3481 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     3541 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     3601 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacacgtg
     3661 gtcgcggcca attctgcagt cgagctcaag cttcgaattc caccggtcgc caccatggtg
     3721 ggcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3781 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3901 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3961 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4081 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4141 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4201 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4321 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaaggatc
     4441 cctcgaggcg gccgcgactc tactggaatt aattcgctgt ctgcgagggc cagctgttgg
     4501 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
     4561 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
     4621 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
     4681 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
     4741 cccaggtcca actgcaggtc gagcatgcat ctagggcggc caattccgcc cctctccccc
     4801 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4861 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4921 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4981 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5041 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5101 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5161 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5221 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5281 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5341 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac
     5401 gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5461 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5521 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5581 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5641 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5701 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5761 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5821 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5881 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5941 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6001 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     6061 gctccgaccg aagccacccg gggcggcccc gccgaccccg cacccgcccc cgaggcccac
     6121 cgactctaga gctcgctgat ccagacatga taagatacat tgatgagttt ggacaaacca
     6181 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6241 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     6301 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     6361 gtatggctga ttatgatccg gctgcctcgc gcttaattaa cccgggggcc gcctgggaac
     6421 tgccgctggc cccccaccgc cccaaggatc tccggtcccc gcccggcttg ctgacgtcac
     6481 ggatgcccca gggtgtgctg ggcaggtagc gggagcctgg gaaatggagt ccattagcag
     6541 aagtggccac ttccaggagt cgctgtgccc cgatgcacac tgggaagtcc gcagctccga
     6601 ggcccccagt ggaaatcgcc agatgagggc ctcctccggg gaatgctggg aaatggagtc
     6661 tacaggccgg aggggtcccc acggcatact agcaagtgtg tgtagcaccg cgtaaagggg
     6721 atgaatagca gactgccccg ggataacttc gtataggata ctttatacga agttatgagc
     6781 tctgtgataa tcactagtga attgatacta gtcggtcacg atatctataa caagaaaata
     6841 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     6901 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     6961 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     7021 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     7081 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaaagct
     7141 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7201 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7261 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9301 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9361 cgaggccctt tcgtc
//
