LOCUS       sequence-419013-        8583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Zhao Y, Pan Z, Hong Z, Sun M, Hong Y, Peng X, Li X, Wang X, Wang K
  TITLE     Protocol for scarless genome editing of human pluripotent stem cell 
            based on orthogonal selective reporters.
  JOURNAL   STAR Protoc. 2024 May 23;5(2):103084. doi: 
            10.1016/j.xpro.2024.103084.
  PUBMED    38787727
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-05-23"; volume: "5(2):103084. doi"; pages: " 
            10.1016/j.xpro.2024.103084"
COMMENT     Sequence Label: SOX17-NLS-tdTomato-EPG
FEATURES             Location/Qualifiers
     source          1..8583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    41..265
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   456..518
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(1414..2002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1494..1513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2173..2964,2965..3033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2796..2815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3034..3138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3206..3224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3809..3826
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3815..3832
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             4103..4159
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4169..4189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4199..4219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4226..5656
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     repeat_region   5668..5702
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    5992..6012
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6013..7191
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6244..7182
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7139..7159
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7201..7797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7201..7220)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             7804..7860
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7861..7863,7864..7866,7867..8580)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7861..7863,7864..7866,7867..8577)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7906..7927)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8167..8186)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8514..8535
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
       61 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      121 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      181 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      241 gctggggatg cggtgggctc tatgggcggc cctgtccctc ataaaagttt tgttacttta
      301 tagaagaaat tttgagtttt tgtttttttt aataaataaa taaacataaa taaattgttt
      361 gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt
      421 aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta cacatgatta
      481 tctttaacgt acgtcacaat atgattatct ttctagggtt aacaggtccc tgatccgccc
      541 cagcctgcag gccagaagca gtgttacaca cttcctggag gagctaagga aatcctcaga
      601 ctcctgggtt tttgttgttg ctgttgttgt tttttaaaag gtgtgttggc atataattta
      661 tggtaattta ttttgtctgc cacttgaaca gtttgggggg gtgaggtttc atttaaaatt
      721 tgttcagaga tttgtttccc atagttggat tgtcaaaacc ctatttccaa gttcaagtta
      781 actagctttg aatgtgtccc aaaacagctt cctccatttc ctgaaagttt attgatcaaa
      841 gaaatgttgt cctgggtgtg ttttttcaat cttctaaaaa ataaaatctg gaatcctgct
      901 tttttgctct actagtacct ctgtcacact agtcttatca aaaaccagtt cttaagatca
      961 atgttaagtt tattagttaa tgtaaatttc tcatcctcga aaagggtgaa cataaatgcc
     1021 tttaaggagt atatctaaaa ataaacatta ggatatctaa gtttgatgta attgtttcag
     1081 gaaggaaaaa agaaaagcat tctggaatga gcctacttca agtaatctta gtttctaaaa
     1141 ctaacagtta atattttcaa ttccagtata tcactttaag tagaagggga tgtccaagta
     1201 attttggttt tctaactgtt gaatcataag cttgacctgc ccccagaggc tttttggatg
     1261 tttttatctg tgttttgcca tctctttaca ctcctcgaca ttcagtttac cttaatcttc
     1321 acatttttac accttgggaa gtggcaagaa ttccatgtga gcaaaaggcc agcaaaaggc
     1381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1441 gcatcacgaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     3181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcggcgcgg
     3241 gtacctgcag gaccacccca actacaagta ccggccgcgg cggcgcaagc aggtgaagcg
     3301 gctgaagcgg gtggagggcg gcttcctgca cggcctggct gagccgcagg cggccgcgct
     3361 gggccccgag ggcggccgcg tggccatgga cggcctgggc ctccagttcc ccgagcaggg
     3421 cttccccgcc ggcccgccgc tgctgcctcc gcacatgggc ggccactacc gcgactgcca
     3481 gagtctgggc gcgcctccgc tcgacggcta cccgttgccc acgcccgaca cgtccccgct
     3541 ggacggcgtg gaccccgacc cggctttctt cgccgccccg atgcccgggg actgcccggc
     3601 ggccggcacc tacagctacg cgcaggtctc ggactacgct ggccccccgg agcctcccgc
     3661 cggtcccatg cacccccgac tcggcccaga gcccgcgggt ccctcgattc cgggcctcct
     3721 ggcgccaccc agcgcccttc acgtgtacta cggcgcgatg ggctcgcccg gggcgggcgg
     3781 cgggcgcggc ttccagatgc agccgcaaca ccagcaccag caccagcacc agcaccaccc
     3841 cccgggcccc ggacagccgt cgccccctcc ggaggcactg ccctgccggg acggcacgga
     3901 ccccagtcag cccgccgagc tcctcgggga ggtggaccgc acggaatttg aacagtatct
     3961 gcacttcgtg tgcaagcctg agatgggcct cccctaccag gggcatgact ccggtgtgaa
     4021 tctccccgac agccacgggg ccatttcctc ggtggtgtcc gacgccagct ccgcggtata
     4081 ttactgcaac tatcctgacg tggcgacgaa ttttagtcta ctgaaacaag cgggagacgt
     4141 ggaggaaaac cctggaccta tcgatggtcc aaaaaagaag agaaaggtat tcgatggtcc
     4201 aaaaaagaag agaaaggtat tcgatatggt gagcaagggc gaggaggtca tcaaagagtt
     4261 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     4321 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     4381 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     4441 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     4501 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     4561 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     4621 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     4681 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     4741 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     4801 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     4861 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
     4921 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
     4981 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     5041 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     5101 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     5161 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     5221 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     5281 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     5341 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     5401 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     5461 cggcggccgc tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     5521 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     5581 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     5641 cgagctgtac aagtaagtcg accttaaccc tagaaagata gtctgcgtaa aattgacgca
     5701 tgcattcttg aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta
     5761 ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc
     5821 actgattttg aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg
     5881 acttttaaga tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat
     5941 aacttattat atatatattt tcttgttata gatatcaggg acagccatca tcaactttgt
     6001 atagaaaagt tgggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     6061 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     6121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     6181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     6241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     6301 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     6361 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     6421 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     6481 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     6541 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     6601 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     6661 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     6721 gctctggtgc ctggtctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     6781 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     6841 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     6901 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     6961 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     7021 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     7081 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     7141 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aaagcttacc
     7201 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     7261 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     7321 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     7381 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     7441 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     7501 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     7561 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     7621 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     7681 gagacctccg cgccccacaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7741 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgct
     7801 agcgccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca
     7861 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     7921 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     7981 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     8041 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     8101 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     8161 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     8221 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     8281 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     8341 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     8401 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     8461 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     8521 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     8581 ctc
//
