LOCUS       sequence-419025-       11574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP11x7-mCherry-alpha-tubulin in insect cells. 5 a. a. 
            linker lengths..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pACUH-GFP11x7-mCherry-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..11574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(127..157,158..359)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    440..534
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    558..652
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        671..909
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     856..877
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             932..1357
                     /codon_start=1
                     /product="seven tandem copies of the 11th beta-strand of 
                     superfolder GFP"
                     /label=GFP11x7
                     /note="each GFP11 associates with GFP(1-10), consisting of 
                     the first 10 beta-strands, to reconstitute fluorescent GFP 
                     (Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDH
                     MVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGITGGSG
                     GRDHMVLHEYVNAAGITGGSGGRDHMVLHEYVNAAGIT"
     CDS             1370..2077
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1515..1533)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1783..1802
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          3550..3615
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3745..3765
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4037..4171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4074..4093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4128..4147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4198..4216)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4668..4739,5141..5414,5489..6143,6205..6520,6724..6855,
                     6926..7540)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8319..8873,8874..8904)
                     /label=P element 5' end
     rep_origin      complement(9139..9727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9219..9238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9898..10689,10690..10758))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10521..10540
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10759..10863)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10931..10949
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10987..11009)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11109..11128
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11338..11407
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
       61 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
      121 ccagaccatg atgaaataac ataaggtggt cccgtcggca agagacatcc acttaacgta
      181 tgcttgcaat aagtgcgagt gaaaggaata gtattctgag tgtcgtattg agtctgagtg
      241 agacagcgat atgattgttg attaaccctt agcatgtccg tggggtttga attaactcat
      301 aatattaatt agacgaaatt atttttaaag ttttattttt aataatttgc gagtacgcaa
      361 agcttggctg catccaacgc gttgggagct ctccggatcc aagctggccg cggctcgatc
      421 cgcttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      481 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaag
      541 cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
      601 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact
      661 ctagcgagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
      721 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
      781 aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa
      841 ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag
      901 agaactctga atagggaatt gggaattcat gcgtgaccac atggtccttc atgagtatgt
      961 aaatgctgct gggattacag gtggctctgg aggtagagat catatggttc tccacgaata
     1021 cgttaacgcc gcaggcatca ctggcggtag tggaggacgc gaccatatgg tactacatga
     1081 atatgtcaat gcagccggaa taaccggagg gtccggaggc cgggatcaca tggtgctgca
     1141 tgagtatgtg aacgcggcgg gtataactgg tgggtcgggc ggacgagacc atatggtgct
     1201 tcacgaatac gtaaacgcag ctggcattac tggcggatca ggtggcaggg atcacatggt
     1261 actccatgag tacgtgaacg ctgctggaat cacaggcggt agcggcggtc gggaccatat
     1321 ggtcctgcac gaatatgtca atgctgccgg tatcaccggc ggcaaattca tggtgagcaa
     1381 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     1441 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     1501 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     1561 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     1621 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1681 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     1741 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     1801 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     1861 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     1921 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     1981 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     2041 ccgccactcc accggcggca tggacgagct gtacaagggt ggaggcggta aattcgttaa
     2101 cagatcttgc ggccgccgtg aatgtatctc tatccatgtt ggtcaggctg gtgtccagat
     2161 tggaaacgcc tgctgggagc tctactgctt ggagcacggc atccagcccg atggccagat
     2221 gccgtctgac aagaccgtgg gcggaggtga tgactcgttc aacaccttct tcagcgagac
     2281 tggagctggc aagcacgtgc cccgcgccgt gtttgtggat ctggaaccca ctgtggtcga
     2341 tgaggtccgt accggaacct accgtcagct gttccacccc gagcagctga tcactggtaa
     2401 ggaggatgcg gccaacaact acgcccgtgg ccactacacc atcggcaagg agatcgtcga
     2461 tctggttctg gacaggatcc gcaagctggc cgatcagtgc accggtctgc agggcttcct
     2521 catcttccac tcgttcggtg gaggtaccgg ctccggcttc acctcgctgc tgatggagcg
     2581 tctctccgtg gactacggca agaagtccaa gctggagttc gccatctacc cagcccccca
     2641 ggtgtccact gccgtggtcg agccctacaa ctccatcctg accacccaca ccaccctgga
     2701 gcattccgac tgcgccttca tggtcgacaa cgaggctatc tacgacatct gccgccgcaa
     2761 tctggacatt gagcgcccca cgtacaccaa cctgaaccgt ctgattggcc agatcgtgtc
     2821 ctcgattacc gcctctctgc gattcgatgg tgcccttaac gtggatctga ctgagttcca
     2881 gaccaacttg gtgccctacc cacgtattca cttccctctg gtgacctacg cccccgttat
     2941 ctccgccgag aaggcctacc acgagcagct gtcggtggct gagatcacca acgcctgctt
     3001 cgagccggcc aaccagatgg tcaagtgcga tccccgtcac ggcaagtaca tggcctgctg
     3061 catgctgtac cgcggtgatg ttgtgcccaa ggacgtcaac gccgctattg ccaccatcaa
     3121 gaccaagcgc accattcaat tcgtcgactg gtgccccact ggcttcaagg ttggcatcaa
     3181 ctaccagcca cccaccgtgg tgcctggagg tgatttggcc aaggtgcagc gtgccgtgtg
     3241 catgttgtcc aacaccacgg ccatcgccga ggcctgggcc cgtctggacc acaagttcga
     3301 tctgatgtac gccaagcgtg ccttcgtcca ctggtacgtt ggtgagggta tggaggaggg
     3361 agagttctcc gaggcccgtg aggatttggc tgccctcgag aaggactacg aggaggtcgg
     3421 catggactcc ggtgacggcg agggtgaggg cgctgaggag tactaatcta gaggatcttt
     3481 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     3541 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     3601 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     3661 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     3721 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     3781 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     3841 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     3901 aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     3961 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4021 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4141 actcatcaat gtatcttatc atgtctggat ccactagaag gcctaattct agtatgtatg
     4201 taagttaata aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact
     4261 gcactggata tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt
     4321 tgatgtacca ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc
     4381 gctgtttgcc tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc
     4441 tatctctttc gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt
     4501 ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg
     4561 ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg
     4621 attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg
     4681 atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg
     4741 tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac
     4801 atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca
     4861 aacaaatagt gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt
     4921 ttgagatcat atcatgatca agacatctaa aggcattcat tttcgactat attctttttt
     4981 acaaaaaata taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa
     5041 agtataaacc tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat
     5101 aacgcttgta gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc
     5161 gcagagctgc attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc
     5221 cagtccgccg gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc
     5281 ctggcacaat atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct
     5341 ggtcaaccgg acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca
     5401 tttgctcaag aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa
     5461 agggtccaat taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg
     5521 gccgtgatgg gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga
     5581 tcgccgcagg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg
     5641 gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc
     5701 tccctaacgg ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg
     5761 acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa
     5821 tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg
     5881 aagcgtctgg cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag
     5941 cccacctccg gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg
     6001 tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag
     6061 ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc
     6121 gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta
     6181 cataacatct caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc
     6241 ggacttttac gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg
     6301 gatcgccaag atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt
     6361 gttggccacc aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa
     6421 ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa
     6481 ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa
     6541 caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc
     6601 ctgattccac acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa
     6661 gataaataaa tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg
     6721 cagatggttg ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt
     6781 gtgatgaata tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc
     6841 tttgccacga taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac
     6901 tttctaatga atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga
     6961 ggcccgaagt cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt
     7021 gccgcttttt ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact
     7081 gcgggccgga gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt
     7141 gtcaacgtcc ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc
     7201 tgtgggtccg ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg
     7261 ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga
     7321 gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc
     7381 gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc
     7441 cgatctgccg ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc
     7501 atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa
     7561 ctgcttgttt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa
     7621 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt
     7681 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt
     7741 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata
     7801 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc
     7861 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt
     7921 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt
     7981 ccgagtggtt catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat
     8041 ttgctccaat atatttgtat atatttccct cacagctata tttattctaa tttaatatta
     8101 tgacttttta aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact
     8161 gaaagtgaca tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata
     8221 atagtgttgt ttatatatat caaaaataac aactataata ataagaatac atttaattta
     8281 gaaaatgctt ggatttcact ggaactagaa ttaattcggc tgctgctcta aacgacgcat
     8341 ttcgtactcc aaagtacgaa ttttttccct caagctctta ttttcattaa acaatgaaca
     8401 ggacctaacg cacagtcacg ttattgttta cataaatgat tttttttact attcaaactt
     8461 actctgtttg tgtactccca ctggtatagc cttcttttat cttttctggt tcaggctcta
     8521 tcactttact aggtacggca tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg
     8581 caggtgcagc cttccactgc gaatcattaa agtgggtatc acaaatttgg gagttttcac
     8641 caaggctgca cccaaggctc tgctcccaca attttctctt aatagcacac ttcggcacgt
     8701 gaattaattt tactccagtc acagctttgc agcaaaattt gcaatatttc attttttttt
     8761 attccacgta agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc
     8821 aacaagcaaa cgtgcactga atttaagtgt atacttcggt aagcttcggc tatcgacggg
     8881 accaccttat gttatttcat catgggccag acccacgtag tccagcggca gatcggcggc
     8941 ggagaagtta agcgtctcca ggatgacctt gcccgaactg gggcacgtgg tgttcgacga
     9001 tgtgcagcta atttcgcccg gctccacgtc cgcccattgg ttaatcagca gaccctcgtt
     9061 ggcgtaacgg aaccatgaga ggtacgacaa ccatttgagg tatactggca ccgagcccga
     9121 gttcaagaag aaggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9541 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9841 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9901 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9961 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10021 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10081 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10141 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10201 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10261 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10321 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10381 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10441 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10501 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10561 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10621 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10681 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10741 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10801 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10861 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10921 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    10981 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11041 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    11101 taactatgcg gcatcagagc agattgtact gagagtgcac catatcgaca tgcccgccgt
    11161 gaccgtcgag aacccgctga cgctgccccg cgtatccgca cccgccgacg ccgtcgcacg
    11221 tcccgtgctc accgtgacca ccgcgcccag cggtttcgag ggcgagggct tcccggtgcg
    11281 ccgcgcgttc gccgggatca actaccgcca cctcgacccg ttcatcatga tggaccagat
    11341 gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcaccgcgg
    11401 cttcgagacc gtgacctaca tcgtcgatat gcggtgtgaa ataccgcacc gaatcgcgcg
    11461 gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg cggagagcgg
    11521 gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa ttat
//
