LOCUS       sequence-419033-       12369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses human MYT1L 
            with G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS
  TITLE     MicroRNA-based conversion of human fibroblasts into striatal medium 
            spiny neurons.
  JOURNAL   Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102. 
            Epub 2015 Sep 17.
  PUBMED    26379228
REFERENCE   2  (bases 1 to 12369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.102"; journalName: "Nat Protoc"; date: 
            "2015-10"; volume: "10"; issue: "10"; pages: "1543-55"
COMMENT     Sequence Label: phMYT1L-N174
FEATURES             Location/Qualifiers
     source          1..12369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..655
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    702..827
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1324..1557
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1742..1786
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2049..2164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2242..2453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3414..3423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7015..7514
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7050..7073)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7447..7466
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7541..8335
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7541..8335
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7595..7614)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8205..8224
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8359..8947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8412..8432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8830..8841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9154..9787
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9915..9931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9915..9931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9928..9950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9939..9955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9963..9969,9970..9987,9988..9993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10008..10029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10146..10163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10317..10905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10397..10416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11076..11867,11868..11936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11699..11718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11937..12041)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12089..12223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12126..12145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12180..12199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
       61 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
      121 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
      181 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
      241 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
      301 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
      361 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
      421 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
      481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      661 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      721 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      781 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      841 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      901 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      961 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1021 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1081 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1141 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1201 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1261 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1321 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1381 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1441 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1501 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1561 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1621 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1681 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1741 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1801 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1861 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1921 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1981 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2041 gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2101 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2161 attttcgggt ttattacagg gacagcagag atccagttta tcgatgaggc cctttcgtct
     2221 tcactcgagg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggatccgcg gccgccacca
     3421 tggaggtgga caccgaggag aagcggcatc gcacgcggtc caaaggggtt cgagttcccg
     3481 tggaaccagc catacaagag ctgttcagct gtcccacccc tggctgtgac ggcagtggtc
     3541 atgtcagtgg caaatatgca agacacagaa gtgtatatgg ttgtcccttg gcgaaaaaaa
     3601 gaaaaacaca agataaacag ccccaggaac ctgctcctaa acgaaagcca tttgccgtga
     3661 aagcagacag ctcctcagtg gatgagtgtg acgacagtga tgggactgag gacatggatg
     3721 agaaggagga ggatgagggg gaggagtact ccgaggacaa tgacgagcca ggggatgagg
     3781 acgaggagga cgaggagggg gaccgggagg aggaggagga gatcgaggag gaggatgagg
     3841 acgatgacga ggatggagaa gatgtggagg atgaagaaga ggaagaggag gaggaggagg
     3901 aggaggaaga ggaagaagaa aacgaagacc atcaaatgaa ttgtcacaat actcgaataa
     3961 tgcaagacac agaaaaggat gataacaata atgacgaata tgacaattac gatgaactgg
     4021 tggccaagtc attgttaaac ctcggcaaaa tcgctgagga tgcagcctac cgggccagga
     4081 ctgagtcaga aatgaacagc aatacctcca atagtctgga agacgatagt gacaaaaacg
     4141 aaaacctggg tcggaaaagt gagttgagtt tagacttaga cagtgatgtt gttagagaaa
     4201 cagtggactc ccttaaacta ttagcccaag gacacggtgt tgtgctctca gaaaacatga
     4261 atgacagaaa ttatgcagac agcatgtcgc agcaagacag tagaaatatg aattacgtca
     4321 tgttggggaa gcccatgaac aatggactca tggaaaagat ggtggaggag agcgatgagg
     4381 aggtgtgtct gagcagtctg gagtgtttga ggaatcagtg cttcgacctg gccaggaagc
     4441 tcagtgagac caacccgcag gagaggaatc cgcagcagaa catgaacatc cgtcagcatg
     4501 tccggccaga agaggacttc ccaggaagga cgccggacag aaactactcg gacatgctga
     4561 acctcatgcg gctggaggag cagttgagcc cccggtcgag agtgtttgcc agctgtgcga
     4621 aggaggatgg gtgtcatgag cgggacgacg ataccacctc tgtgaactcg gacaggtctg
     4681 aagaggtgtt cgacatgacc aaggggaacc tgaccctgct ggagaaagcc atcgctttgg
     4741 aaacggaaag agcaaaggcc atgagggaga agatggccat ggaagctggg aggagggaca
     4801 atatgaggtc atatgaggac cagtctccga gacaacttcc cggggaggac agaaagccta
     4861 aatccagtga cagccatgtc aaaaagccat actatgatcc ctcaagaaca gaaaagaaag
     4921 agagcaagtg tccaaccccc gggtgtgatg gaaccggcca cgtaactggg ctgtacccac
     4981 atcaccgcag cctgtccgga tgcccgcaca aagatagggt ccctccagaa atccttgcca
     5041 tgcatgaaag tgtcctcaag tgccccactc cgggctgcac ggggcgcggg catgtcaaca
     5101 gcaacaggaa ctcccaccga agcctctccg gatgcccgat cgctgcagca gagaaactgg
     5161 ccaaggcaca ggaaaagcac cagagctgcg acgtgtccaa gtccagccag gcctcggacc
     5221 gcgtgctcag gccaatgtgc tttgtgaagc agctggagat tcctcagtat ggctacagaa
     5281 acaatgtccc cacaactacg ccgcgttcca acctggccaa ggagctcgag aaatattcca
     5341 agacctcgtt tgaatacaac agttacgaca accatactta tggcaagcga gccatagctc
     5401 ccaaggtgca aaccagggat atatccccca aaggatatga tgatgcgaag cggtactgca
     5461 aggaccccag ccccagcagc agcagcacca gcagctacgc gcccagcagc agcagcaacc
     5521 tgagctgcgg cgggggcagc agcgccagca gcacgtgcag caagagcagc ttcgactaca
     5581 cgcacgacat ggaggcggcc cacatggcgg ccaccgccat cctcaacctg tccacgcgct
     5641 gccgcgagat gccgcagaac ctgagcacca agccgcagga cctgtgcgcc acgcggaacc
     5701 ctgacatgga ggtggatgag aacgggaccc tggacctcag catgaacaag cagaggccgc
     5761 gggacagctg ctgccccatc ctgacccctc tggagcccat gtccccccag cagcaggcag
     5821 tgatgaacaa ccggtgtttc cagctgggcg agggcgactg ctgggacttg cccgtagact
     5881 acaccaaaat gaaaccccgg aggatagacg aggacgagtc caaagacatt actccagaag
     5941 acttggaccc attccaggag gctctagaag aaagacggta tcccggggag gtgaccatcc
     6001 caagtcccaa acccaagtac cctcagtgca aggagagcaa aaaggactta ataactctgt
     6061 ctggctgccc cctggcggac aaaagcattc gaagtatgct ggccaccagc tcccaagaac
     6121 tcaagtgccc cacgcctggc tgtgatggtt ctggacatat caccggcaat tatgcttctc
     6181 atcggagcct ttcaggttgc ccaagagcaa agaaaagtgg tatcaggata gcacagagca
     6241 aagaagataa agaagatcaa gaacccatca ggtgtccggt ccccgggtgc gacggccagg
     6301 gccacatcac tgggaagtac gcgtcccatc gcagcgcctc cgggtgcccc ttggcggcca
     6361 agaggcagaa agacgggtac ctgaatggct cccagttctc ctggaagtcg gtcaagacgg
     6421 aaggcatgtc ctgccccacg ccaggatgcg acggctcagg ccacgtcagc ggcagcttcc
     6481 tcacacaccg cagcttgtca ggatgcccga gagccacgtc agcgatgaag aaggcaaagc
     6541 tttctggaga gcagatgctg accatcaaac agcgggccag caacggtata gaaaatgatg
     6601 aagaaatcaa acagttagat gaagaaatca aggagctaaa tgaatccaat tcccagatgg
     6661 aagccgatat gattaaactc agaactcaga ttaccacgat ggagagcaac ctgaagacca
     6721 tcgaagagga gaacaaagtg attgagcagc agaacgagtc tctcctccac gagctggcga
     6781 acctgagcca gtctctgatc cacagcctgg ctaacatcca gctgccgcac atggatccaa
     6841 tcaatgaaca aaattttgat gcttacgtga ctactttgac ggaaatgtat acaaatcaag
     6901 atcgttatca gagtccagaa aataaagccc tactggaaaa tataaagcag gctgtgagag
     6961 gaattcaggt ctgaggccgc gccggctcta gatcgcgaac gcgtgaattc taccgggtag
     7021 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     7081 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     7141 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     7201 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     7261 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     7321 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     7381 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     7441 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     7501 ctccgggcct ttcgacctgc agcccaagct tcctaggacc atgattgaac aagatggatt
     7561 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7621 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7681 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     7741 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7801 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7861 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7921 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7981 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     8041 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     8101 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     8161 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8221 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8281 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgacccgg
     8341 gcggggcgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8761 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8881 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8941 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     9001 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     9061 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     9121 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     9181 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     9241 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     9301 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     9361 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     9421 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     9481 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     9541 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     9601 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9661 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9721 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9781 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9841 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     9901 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9961 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10021 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10081 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10141 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10201 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10261 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10741 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10981 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11041 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12061 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    12121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    12241 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12301 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12361 ttgttaaat
//
