LOCUS       sequence-419038-        9253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pJFRC19-13XLexAop2-IVS-myr::GFP
FEATURES             Location/Qualifiers
     source          1..9253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(113..701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(193..212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(872..1663,1664..1732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1495..1514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1733..1837)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1904..1922
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    1964..2098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1988..2007)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2042..2061
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2370..2390)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          2520..2585
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(2672..3385)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          3670..3736
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     promoter        complement(3751..3989)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(3783..3804)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             complement(join(5643..6257,6328..6459,6663..6978,7040..7694,
                     7769..8042,8444..8515))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8967..8985
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    join(9210..9253,1..26)
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacggt aacatgtgag
       61 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      121 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      181 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      241 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      301 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      361 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      421 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      481 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      541 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      601 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      661 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      721 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      781 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      841 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
      901 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
      961 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1021 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1081 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1141 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1201 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1261 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1321 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1381 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1441 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1501 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1561 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1621 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1681 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1741 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1801 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1861 ctgagtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1921 gccctttcgt cttcaagaat tcgtttaaac ggccggccag atcgatccag acatgataag
     1981 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2041 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2101 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2161 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta
     2221 tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg
     2281 taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc
     2341 aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa
     2401 tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg
     2461 ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc
     2521 taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata
     2581 tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag
     2641 taaggttcct tcacaaagat cctctagatt acttgtagag ctcatccatg ccgtgtgtaa
     2701 taccagctgc ggtcacaaac tccagcaaca ccatgtggtc ccgcttttca ttggggtcct
     2761 tgctcaacgc ggactgtgtg gaaaggtagt gattgtctgg caagagaact gggccatctc
     2821 caatgggtgt gttctgttgg taatgatctg caagttgcac ggaaccgtcc tctatgttgt
     2881 ggcgaatctt gaagttaacc tttattccgt ttttctgctt atctgccatt atatacacat
     2941 tatgcgagtt gtagttgtat tccaacttat ggcccagaat gttgccatcc tccttaaaat
     3001 caatgccctt caactcaata cggttaacga gtgtgtcgcc ttcaaatttc acctccgctc
     3061 gcgtcttata gttgccgtca tccttgaaga aaatggtcct ctcttgaacg tatccctccg
     3121 gcatcgccga cttgaaaaag tcatgctgct tcatatgatc ggggtaacgg ctgaaacact
     3181 ggacaccata cgtcagcgtc gtgaccaacg tcggccatgg cacgggcagc ttgcccgtgg
     3241 tgcatatgaa cttcaacgtc agcttaccgt atgtggcgtc gccctcaccc tcgccggaca
     3301 cactgaattt atgaccgttg acatcgccgt cgagttcaac caatatcggg actactccgg
     3361 taaacagttc ttcacctttg gacatggatc cgcgtggtct gatgatgtca acgccgccct
     3421 ttccgccgat tttcaacgga ccccgatgat tgggatcggg cgtgtagcgc gagtccagcg
     3481 agccaccgtt gttatgcttc tggccaagaa cgccgcccat gttgccgccg ccgctgctgt
     3541 tgatgtgcgt ctggccaaag gtgtagtcgg atttcttgta gcccccagtg ggataggcca
     3601 gtgccagttc ctgatcctgt cgcttgctgc agcatttgtt gcccattttg attctcgagc
     3661 cgcggccgcc tgaagtaaag gataagaatt agggttagtc gtttcggtgt gcctaggcat
     3721 cagtggttga acctaccttt tagatctatt cagagttctc ttcttgtatt caataattac
     3781 ttcttggcag atttcagtag ttgcagttga tttacttggt tgctggttac ttttaattga
     3841 ttcactttaa cttgcacttt actgcagatt gtttagcttg ttcagctgcg cttgtttatt
     3901 tgcttagctt tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg
     3961 tagacgaagc gcctctattt atactccggc gctcgacgtc actagtgcta gagtctccgc
     4021 ttactgtatg gatgtacagt acttactgta tggatgtaca gtacttactg tatggatgta
     4081 cagtacttac tgtatggatg tacagtactt actgtatgga tgtacagtaa cctgcaggca
     4141 tgcgctagcg ctagagtctc cgcttactgt atggatgtac agtacttact gtatggatgt
     4201 acagtactta ctgtatggat gtacagtaac ctgcaggcat gccctagggc tagagtctcc
     4261 gcttactgta tggatgtaca gtacttactg tatggatgta cagtacttac tgtatggatg
     4321 tacagtactt actgtatgga tgtacagtac ttactgtatg gatgtacagt aacctgcagg
     4381 catgcgctag cgctagagtc tccgcttact gtatggatgt acagtactta ctgtatggat
     4441 gtacagtact tactgtatgg atgtacagta acctgcaggc atgccctagg gctagagtct
     4501 ccgcttactg tatggatgta cagtacttac tgtatggatg tacagtactt actgtatgga
     4561 tgtacagtac ttactgtatg gatgtacagt acttactgta tggatgtaca gtaacctgca
     4621 ggcatgcaag cttgcggccc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     4681 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     4741 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     4801 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
     4861 tccggaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa atgtattctt
     4921 attattatag ttgttatttt tgatatatat aaacaacact attatgccca ccattttttt
     4981 gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa ttgtaacgct
     5041 aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta aattagaata
     5101 aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg tacatacaaa
     5161 tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt gagcgaaccg
     5221 aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc
     5281 ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat
     5341 tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat
     5401 gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag
     5461 acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc
     5521 atatcttgtt tttattggca caaatataat tacattagct ttttgagggg gcaataaaca
     5581 gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc
     5641 ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc
     5701 acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc
     5761 gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt
     5821 tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac
     5881 catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag
     5941 ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc catcgaggtc
     6001 gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc caccagagtg
     6061 accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat catcggatag
     6121 gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc aatcgttttg
     6181 cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat aaaaactggc
     6241 agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa attattgaaa
     6301 tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt gaaaggtcat
     6361 gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt gcgtgagttg
     6421 ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa aatgttactc
     6481 gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa aactataaac
     6541 tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct atctaaaatc
     6601 tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact ggaaccactc
     6661 accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac cgacagccag
     6721 gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt gtacccattc
     6781 tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc catatcccgg
     6841 gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg ggactcgatc
     6901 tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt gtagttggta
     6961 ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta gataccctta
     7021 ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag tgcccaagaa
     7081 agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca gctcggaaga
     7141 cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct tcagcacctg
     7201 gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc agatcagaag
     7261 cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc cgccggacag
     7321 acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga gcgaaagctc
     7381 ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc gtggcatccg
     7441 caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa agaggtcatc
     7501 ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt ggccattgag
     7561 cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg caagggcatt
     7621 cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt cgcccggata
     7681 ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag atttcacaca
     7741 gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct gggcgccggt
     7801 atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg ccatccggag
     7861 cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt gaggttctcg
     7921 gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg gagcgtgccg
     7981 tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc tccgctgtca
     8041 ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat ctaaccccga
     8101 acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta ttttttgtgc
     8161 atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga atatagtcga
     8221 aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc ttatatagcg
     8281 agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat tgcggtttgg
     8341 gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat cgcagttcct
     8401 atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc agatgctcgg
     8461 cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg cccattgccg
     8521 ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc caatcactca
     8581 aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt gtttaattga
     8641 tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac tgaaggcgga
     8701 cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa
     8761 gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt tgagtggaat
     8821 gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct tggatcgaag
     8881 taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt
     8941 tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata ctaggcgcgc
     9001 ccatatgttc ggcttgtcga catgcccgcc gtgaccgtcg agaacccgct gacgctgccc
     9061 cgcgtatccg cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc
     9121 agcggtttcg agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat caactaccgc
     9181 cacctcgacc cgttcatcat gatggaccag atgggtgagg tggagtacgc gcccggggag
     9241 cccaagggca cgc
//
