LOCUS       sequence-419052-        9943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for knock-in GFP-CD19 into the human INS locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Zhao Y, Pan Z, Hong Z, Sun M, Hong Y, Peng X, Li X, Wang X, Wang K
  TITLE     Protocol for scarless genome editing of human pluripotent stem cell 
            based on orthogonal selective reporters.
  JOURNAL   STAR Protoc. 2024 May 23;5(2):103084. doi: 
            10.1016/j.xpro.2024.103084.
  PUBMED    38787727
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2024-05-23"; volume: "5(2):103084. doi"; pages: " 
            10.1016/j.xpro.2024.103084"
COMMENT     Sequence Label: INS-GFP-CD19-EPT
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(68..85)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    74..298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   489..551
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(1629..2217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1709..1728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2388..3179,3180..3248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3011..3030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3249..3353)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3421..3439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             4449..4505
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4506..4508,4509..4511,4512..5222)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4506..4508,4509..4511,4512..5222)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4551..4572)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4812..4831)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5159..5180
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5223..5276
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     repeat_region   6290..6324
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    6614..6634
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6635..7813
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6866..7804
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7761..7781
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7823..8419
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7823..7842)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             8426..8482
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8492..8512
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8522..8542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(8549..9943,1..36)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ctgttcctgt acggcatgga cgagctgtac aagtaatcta gagggcccgt ttaaacccgc
       61 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      121 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      181 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
      241 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggc
      301 ggccctgtcc ctcataaaag ttttgttact ttatagaaga aattttgagt ttttgttttt
      361 tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg
      421 taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg
      481 ataaaacaca tgcgtcaatt ttacacatga ttatctttaa cgtacgtcac aatatgatta
      541 tctttctagg gttaaacgca gcccgcaggc agccccccac ccgccgcctc ctgcaccgag
      601 agagatggaa taaagccctt gaaccagccc tgctgtgccg tctgtgtgtc ttgggggccc
      661 tgggccaagc cccacttccc ggcactgttg tgagcccctc ccagctctct ccacgctctc
      721 tgggtgccca caggtgccaa cgccggccag gcccagcatg cagtggctct ccccaaagcg
      781 gccatgcctg tcggctgcct gctgccccca ccctgtggct cagggtccag tatgggagct
      841 gcgggggtct ctgaggggcc aggggtggtg gggccactga gaagtgactt cttgttcagt
      901 agctctggac tcttggagtc cccagagacc ttgttcagga aagggaatga gaacattcca
      961 gcaattttcc ccccacctag ccctcccagg ttctattttt agagttattt ctgatggagt
     1021 ccctgtggag ggaggaggct gggctgaggg agggggtcct gcagggcggg gggctgggaa
     1081 ggtggggaga ggctgccgag agccacccgc tatccccagc tctgggcagc cccgggacag
     1141 tcacacaccc tggcctcgcg gcccaagctg gcagccgtct gcagccacag cttatgccag
     1201 cccaggtcca gccagacacc tgagggaccc actggtgcct tggaggaagc aggagaggtc
     1261 agatggcacc atgagctggg gcaggtgcag ggaccgtggc agcacctggc agggcctcag
     1321 aacccatgcc ttgggcaccc cggccatgag gccctgagga ttgcagccca ggagaagcag
     1381 ggaaccgcca gggccacagg ggcagagacc agggccaggg tccccctgca gccccttagc
     1441 ccaccccctc ccagtaagca gggctgcttg gctggcttcc tttgctacag acctgctgct
     1501 cacccagaag ggcccacggg ccctggtgac aaggtcgttg tggctccagg tcaactctct
     1561 ctgaattcca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1621 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1681 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1741 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1801 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1861 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1921 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1981 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2041 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2101 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2161 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2221 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2281 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2341 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2401 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2461 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2521 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2581 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2641 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2701 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2761 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2821 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2881 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2941 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3001 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3061 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3361 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3421 aataggcgta tcacgaggcc ctttcgtcgg cgcgggtacc ggggaacgag gcttcttcta
     3481 cacacccaag acccgccggg aggcagagga cctgcagggt gagccaaccg cccattgctg
     3541 cccctggccg cccccagcca ccccctgctc ctggcgctcc cacccagcat gggcagaagg
     3601 gggcaggagg ctgccaccca gcagggggtc aggtgcactt ttttaaaaag aagttctctt
     3661 ggtcacgtcc taaaagtgac cagctccctg tggcccagtc agaatctcag cctgaggacg
     3721 gtgttggctt cggcagcccc gagatacatc agagggtggg cacgctcctc cctccactcg
     3781 cccctcaaac aaatgccccg cagcccattt ctccaccctc atttgatgac cgcagattca
     3841 agtgttttgt taagtaaagt cctgggtgac ctggggtcac agggtgcccc acgctgcctg
     3901 cctctgggcg aacaccccat cacgcccgga ggagggcgtg gctgcctgcc tgagtgggcc
     3961 agacccctgt cgccaggcct cacggcagct ccatagtcag gagatgggga agatgctggg
     4021 gacaggccct ggggagaagt actgggatca cctgttcagg ctcccactgt gacgctgccc
     4081 cggggcgggg gaaggaggtg ggacatgtgg gcgttggggc ctgtaggtcc acacccactg
     4141 tgggtgaccc tccctctaac ctgggtccag cccggctgga gatgggtggg agtgcgacct
     4201 agggctggcg ggcaggcggg cactgtgtct ccctgactgt gtcctcctgt gtccctctgc
     4261 ctcgccgctg ttccggaacc tgctctgcgc ggcacgtcct ggcagtgggg caggtggagc
     4321 tgggcggggg ccctggtgca ggcagcctgc agcccttggc cctggagggg tccctgcaga
     4381 agcgtggcat tgtggaacaa tgctgtacca gcatctgctc cctctaccag ctggagaact
     4441 actgcaacgc gactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg
     4501 ggccaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4561 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4621 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     4681 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     4741 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4801 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4861 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4921 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4981 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     5041 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     5101 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     5161 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     5221 aggagggcag gggcagcctg ctgacctgcg gcgacgtgga ggagaacccc ggccccatgc
     5281 ccccccccag gctgctgttc ttcctgctgt tcctgacccc catggaggtg aggcccgagg
     5341 agcccctggt ggtgaaggtg gaggagggcg acaacgccgt gctgcagtgc ctgaagggca
     5401 ccagcgacgg ccccacccag cagctgacct ggagcaggga gagccccctg aagcccttcc
     5461 tgaagctgag cctgggcctg cccggcctgg gcatccacat gaggcccctg gccatctggc
     5521 tgttcatctt caacgtgagc cagcagatgg gcggcttcta cctgtgccag cccggccccc
     5581 ccagcgagaa ggcctggcag cccggctgga ccgtgaacgt ggagggcagc ggcgagctgt
     5641 tcaggtggaa cgtgagcgac ctgggcggcc tgggctgcgg cctgaagaac aggagcagcg
     5701 agggccccag cagccccagc ggcaagctga tgagccccaa gctgtacgtg tgggccaagg
     5761 acaggcccga gatctgggag ggcgagcccc cctgcctgcc ccccagggac agcctgaacc
     5821 agagcctgag ccaggacctg accatggccc ccggcagcac cctgtggctg agctgcggcg
     5881 tgccccccga cagcgtgagc aggggccccc tgagctggac ccacgtgcac cccaagggcc
     5941 ccaagagcct gctgagcctg gagctgaagg acgacaggcc cgccagggac atgtgggtga
     6001 tggagaccgg cctgctgctg cccagggcca ccgcccagga cgccggcaag tactactgcc
     6061 acaggggcaa cctgaccatg agcttccacc tggagatcac cgccaggccc gtgctgtggc
     6121 actggctgct gaggaccggc ggctggaagg tgagcgccgt gaccctggcc tacctgatct
     6181 tctgcctgtg cagcctggtg ggcatcctgc acctgcagag ggccctggtg ctgaggagga
     6241 agaggaagag gatgaccgac cccaccagga ggttctgagt cgaccttaac cctagaaaga
     6301 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     6361 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     6421 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     6481 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     6541 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcag
     6601 ggacagccat catcaacttt gtatagaaaa gttgggctcc ggtgcccgtc agtgggcaga
     6661 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     6721 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     6781 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     6841 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     6901 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     6961 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     7021 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     7081 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     7141 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     7201 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     7261 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     7321 gtagtctcaa gctggccggc ctgctctggt gcctggtctc gcgccgccgt gtatcgcccc
     7381 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     7441 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     7501 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     7561 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     7621 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     7681 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     7741 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     7801 tcaggtgtcg tgaaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7861 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7921 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7981 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     8041 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     8101 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     8161 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     8221 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8281 cgccggggtg cccgccttcc tggagacctc cgcgccccac aacctcccct tctacgagcg
     8341 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8401 gacccgcaag cccggtgccg ctagcgcgac gaattttagt ctactgaaac aagcgggaga
     8461 cgtggaggaa aaccctggac ctatcgatgg tccaaaaaag aagagaaagg tattcgatgg
     8521 tccaaaaaag aagagaaagg tattcgatat ggtgagcaag ggcgaggagg tcatcaaaga
     8581 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     8641 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     8701 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     8761 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     8821 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     8881 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     8941 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     9001 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     9061 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     9121 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     9181 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     9241 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     9301 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     9361 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     9421 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     9481 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     9541 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     9601 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     9661 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     9721 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     9781 ggacggcggc cgctacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     9841 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     9901 caccatcgtg gaacagtacg agcgctccga gggccgccac cac
//
