LOCUS       sequence-419069-        8102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Histone H3.3K36M-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Schibler AC, Jevtic P, Pegoraro G, Levy DL, Misteli T
  TITLE     Identification of epigenetic modulators as determinants of nuclear 
            size and shape.
  JOURNAL   Elife. 2023 May 23;12:e80653. doi: 10.7554/eLife.80653.
  PUBMED    37219077
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-05-23"; volume: "12:e80653. doi"; pages: " 
            10.7554/eLife.80653"
COMMENT     Sequence Label: pACS145
FEATURES             Location/Qualifiers
     source          1..8102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3040..3081
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3105..3693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3158..3178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3576..3587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3708..4024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3875..4010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4072..4119
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4138..4536
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4623..4856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4928..5049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4965..4984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5019..5038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5089..5224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5244..5263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5245..5263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5273..5290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5273..5289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5282..5304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5431..5886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5518..5537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5728..5749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5912..6016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6017..6085,6086..6877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6235..6254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7048..7636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7537..7556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7790..7807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7924..7945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7960..7965,7966..7983,7984..7990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7998..8014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8003..8025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8022..8038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8022..8038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8057..8077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8059..8077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattggccc ttatggctcg tacaaagcag
     2581 actgcccgca aatcgaccgg tggtaaagca cccaggaagc aactggctac aaaagccgct
     2641 cgcaagagtg cgccctctac tggaggggtg atgaaacctc atcgttacag gcctggtact
     2701 gtggcgctcc gtgaaattag acgttatcag aagtccactg aacttctgat tcgcaaactt
     2761 cccttccagc gtctggtgcg agaaattgct caggacttta aaacagatct gcgcttccag
     2821 agcgcagcta tcggtgcttt gcaggaggca agtgaggcct atctggttgg cctttttgaa
     2881 gacaccaacc tgtgtgctat ccatgccaaa cgtgtaacaa ttatgccaaa agacatccag
     2941 ctagcacgcc gcatacgtgg agaacgtgct gcaagggcca attctgcaga tatccagcac
     3001 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     3061 ctcctcggtc tcgattctac gcgtaccggt tagtaatgat cgacaatcaa cctctggatt
     3121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     3541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3661 cgagtcggat ctccctttgg gccgcctccc cgcctggcga tggtaccggt gtggaaagtc
     3721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3841 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3961 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4021 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     4081 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     4141 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     4201 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     4261 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     4321 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     4381 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     4441 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     4501 gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct cggtaccttt
     4561 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4621 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4681 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4741 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4801 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4861 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4921 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5041 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5101 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5161 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5221 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     5281 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5341 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5401 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     5461 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5521 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5581 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5641 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5701 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5761 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5821 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5881 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6001 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6061 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6121 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6181 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6241 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6301 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6361 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6421 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6481 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6541 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6601 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6661 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6721 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6781 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6841 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6901 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6961 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7561 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7621 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7681 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7741 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7801 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7861 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7921 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7981 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8041 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8101 tt
//
