LOCUS       sequence-419128-       15624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RUBY under the control of Ubiquitin promoter/marker for 
            transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15624)
  AUTHORS   He Y, Zhang T, Sun H, Zhan H, Zhao Y
  TITLE     A reporter for noninvasively monitoring gene expression and plant 
            transformation.
  JOURNAL   Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. 
            eCollection 2020.
  PUBMED    33024566
REFERENCE   2  (bases 1 to 15624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2020-09-19"; volume: "7:152. doi"; pages: " 
            10.1038/s41438-020-00390-1. eCollection 2020"
COMMENT     Sequence Label: UBQ:RUBY
FEATURES             Location/Qualifiers
     source          1..15624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    133..157
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2146..6096
                     /label=RUBY
     CDS             3646..3702
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4537..4593
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        6800..6979
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6832..7015
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      8130..8382
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8957..8974)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8957..8973)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8966..8988)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9195..9219
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9740..10531
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10777..11365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11266..11285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11519..11536
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11551..11691
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11617..11636)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11777..11799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12035..12229
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12295..13368)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13797..14426)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
       61 tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct
      121 cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt tctgcagtgc
      181 agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa
      241 aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac
      301 atatatttaa actttactct acgaataata taatctatag tactacaata atatcagtgt
      361 tttagagaat catataaatg aacagttaga catggtctaa aggacaattg agtattttga
      421 caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa
      481 tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt
      541 taatggtttt tatagactaa tttttttagt acatctattt tattctattt tagcctctaa
      601 attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga
      661 ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga
      721 aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa
      781 cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc
      841 atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgcgtg
      901 gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg
      961 cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat
     1021 aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac
     1081 acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc
     1141 gtcctccccc cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc
     1201 cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg
     1261 ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca
     1321 gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc
     1381 atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata
     1441 tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga
     1501 tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt
     1561 ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa
     1621 gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat
     1681 gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc
     1741 gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat
     1801 tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa
     1861 tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg
     1921 catatgcagc atctattcat atgctctaac cttgagtacc tatctattat aataaacaag
     1981 tatgttttat aattattttg atcttgatat acttggatga tggcatatgc agcagctata
     2041 tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg
     2101 tcgatgctca ccctgttgtt tggtgttact tctgcagaaa ctagtatgga tcatgcgacc
     2161 ctcgccatga tcctcgcgat ctggttcatc agcttccact tcatcaagct gctgttctcc
     2221 cagcagacca ccaagctgct tccgccagga ccaaagccgc ttccgatcat cggcaacatc
     2281 cttgaggtgg gcaagaagcc gcatcggtcc ttcgccaacc tcgccaagat tcacggccca
     2341 ctcatttccc tcagactcgg ctctgtgacc accatcgttg tgtcctctgc cgacgtggcc
     2401 aaagagatgt tcctcaagaa ggatcacccg ctctccaacc gcacgatccc gaatagtgtt
     2461 acagccggcg accaccacaa gctcaccatg tcttggctcc cggtgtctcc gaagtggcgc
     2521 aacttccgca agattaccgc cgtgcatctg ctctccccac agagactcga tgcctgccag
     2581 acattcaggc acgccaaggt gcagcagctc tacgagtacg ttcaagagtg cgcccagaaa
     2641 ggccaggccg tggatattgg caaggccgcc tttacgacca gcctcaacct cctcagcaag
     2701 ctgttcttca gcgtcgagct ggcgcaccac aagtcccata ccagccaaga gttcaaagag
     2761 ctgatctgga acatcatgga agatataggc aagccgaact acgccgacta cttcccgatt
     2821 ctcggctgcg ttgacccatc tggcattaga agaaggctcg cctgctcctt cgacaagctg
     2881 atcgccgtgt tccagggcat catctgcgag agactcgccc cagattcctc caccacaact
     2941 accaccacca ccgacgacgt gctcgatgtg ctcctccagc tgttcaagca gaacgagctg
     3001 acgatgggcg agatcaacca cctcctcgtg gacatcttcg acgccggcac cgataccaca
     3061 tcctccacat tcgagtgggt gatgaccgag ctgatccgca atccagagat gatggaaaag
     3121 gcccaagagg aaatcaagca ggtcctcggc aaggacaagc agatccaaga gtccgacatc
     3181 atcaacctgc cgtacctcca ggcgatcatc aaagagacac tccgcctcca tccgccgacc
     3241 gtgttcttgc tcccaagaaa ggccgacacc gatgtcgagc tgtacggcta catcgtgccg
     3301 aaggatgccc agatcctcgt gaacctctgg gccattggca gggacccaaa cgcctggcag
     3361 aacgccgata ttttcagccc agagcgcttc atcggctgcg agatcgatgt taagggccgc
     3421 gatttcggcc tccttccatt tggcgctggc cgcagaattt gcccaggcat gaatctcgcc
     3481 atcaggatgc tcaccctcat gctcgccaca ctcctccagt tcttcaactg gaagctcgaa
     3541 ggcgacatct ccccgaagga cctcgacatg gacgagaagt tcggcattgc gctccaaaag
     3601 accaagccgc tcaagctcat cccgattccg cgctacggta gcggagctac caattttagc
     3661 ctccttaagc aggcaggtga tgtagaagag aaccccgggc ctatgaagat gatgaacggc
     3721 gaggacgcca acgaccagat gatcaaagag tccttcttca tcacccacgg caacccgatc
     3781 ctcaccgtcg aggatacaca tccgctcagg ccgttcttcg agacatggcg cgagaagatt
     3841 ttctccaaga agccgaaggc catcctcatc atctccggcc actgggagac agtgaagcca
     3901 accgtgaacg ccgtgcacat caacgacacc atccacgact tcgacgacta cccagccgcc
     3961 atgtaccagt tcaagtaccc agctccaggc gagccagagc ttgcgagaaa ggtggaagag
     4021 atcctcaaga agtccgggtt cgagacagcc gagacagacc aaaagagggg ccttgatcac
     4081 ggcgcctggg ttccactcat gctcatgtat ccagaggcgg acatcccggt gtgccagctc
     4141 tcagttcagc cacatctcga cggcacctac cactacaatc tcggcagagc cctcgcgccg
     4201 ctcaagaatg atggcgtgct cattattggc tccggcagcg ccacacatcc actcgatgag
     4261 acaccgcact acttcgatgg tgttgcccct tgggccgctg ccttcgattc ttggcttagg
     4321 aaggccctca tcaacggccg cttcgaggaa gtgaacatct acgagagcaa ggccccgaac
     4381 tggaagctcg cccatccatt tccagagcac ttctacccgc tccacgttgt gctcggcgct
     4441 gctggtgaaa agtggaaggc cgagctgatc cactcctcct gggatcatgg cacactttgc
     4501 cacggctcct acaagttcac ctccgccgga tccggagcaa ccaactttag cctgctcaag
     4561 caagcaggag atgttgagga aaatcctggc cccatgaccg ccatcaagat gaacaccaac
     4621 ggcgagggcg agacacagca catcctcatg atcccgttca tggcgcaggg ccacctcagg
     4681 ccatttctcg aactcgccat gttcctctac aagcgctccc acgtgatcat caccctgctc
     4741 acaactccgc tcaatgccgg cttcctcagg cacctccttc accaccattc ctactcctcc
     4801 agcggcatca ggatcgtcga gctgccattc aactccacca accacggact cccaccgggc
     4861 atcgagaaca ccgataagct cacactcccg ctcgtggtgt ccctcttcca ttccaccatc
     4921 agcctcgatc cgcacctccg cgattacatc tccaggcatt tcagcccagc caggccacca
     4981 ctctgcgtga tccatgatgt gttcctcggc tgggttgacc aggtggccaa ggatgtgggc
     5041 tctacaggcg tggtgttcac aacaggcggc gcttatggca catccgccta cgtgtccatc
     5101 tggaacgatc tcccgcacca gaactactcc gacgaccaag agttcccgct gccaggcttc
     5161 ccagagaacc ataagttccg caggtcccag ctccatcggt tcctcagata tgccgacggc
     5221 tccgacgatt ggtccaagta tttccagccg cagctccgcc agtccatgaa gtcttttggc
     5281 tggctctgca actccgtgga agagatcgag acactcggct tctccatcct ccgcaactac
     5341 accaagctgc cgatctgggg catcggccca cttattgctt ccccagtgca gcactcctcc
     5401 tccgacaaca attcaacagg cgccgagttc gtgcagtggc tcagcctcaa agagccggac
     5461 tccgtcctct acatctcctt cggctcccag aacacgatca gcccgacgca gatgatggaa
     5521 ctcgctgctg gccttgagtc ctccgagaag ccattcctct gggtgatcag agccccgttc
     5581 ggcttcgaca tcaacgaaga gatgcgccca gagtggctgc cagagggctt tgaggaacgc
     5641 atgaaggtga agaaacaggg caagctcgtg tacaagctcg gcccgcagct tgagatcctc
     5701 aaccatgaat ccatcggcgg ctttctcacc cactgcggat ggaacagcat ccttgagtct
     5761 cttcgcgagg gcgttccgat gcttggatgg ccacttgctg ccgagcaggc ctacaacctc
     5821 aagtacctcg aagatgagat gggcgtcgcg gttgagcttg ctagaggcct cgaaggcgag
     5881 atctccaaag agaaggtcaa gcgcatcgtc gagatgatcc ttgagcgcaa cgagggctcc
     5941 aaaggctggg agatgaagaa tcgcgccgtg gaaatgggca aaaagctcaa ggacgccgtg
     6001 aacgaggaaa aagagctgaa gggctcctcc gtgaaggcga tcgacgattt cctcgacgcc
     6061 gtcatgcagg ccaaacttga gccaagcctc cagtgatagt gaatatgaag atgaagatga
     6121 aatatttggt gtgtcaaata aaaaggttgt gtgcttaagt ttgtgttttt ttcttggctt
     6181 gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaacatc tcattataat
     6241 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctccagcat
     6301 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata agacctaact
     6361 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     6421 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     6481 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     6541 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     6601 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     6661 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     6721 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     6781 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     6841 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     6901 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     6961 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     7021 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     7081 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     7141 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatatag gagggcgtgg
     7201 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     7261 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     7321 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     7381 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     7441 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     7501 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     7561 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     7621 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     7681 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     7741 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     7801 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     7861 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     7921 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     7981 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     8041 cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcttggaggt gatgtaacat
     8101 gatcacaagc tgatcccccg aatttccccg atcgttcaaa catttggcaa taaagtttct
     8161 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     8221 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     8281 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     8341 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     8401 gacagcttgc atgccagctt gggctgcagg tcgacgcgtc aattgatatc gatccaggcc
     8461 tcccagcttt cgtccgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt
     8521 tcattgccca cacaccagaa tcctactaag tttgagtatt atggcattgg aaaagctgtt
     8581 ttcttctatc atttgttctg cttgtaattt actgtgttct ttcagttttt gttttcggac
     8641 atcaaaatgc aaatggatgg ataagagtta ataaatgata tggtcctttt gttcattctc
     8701 aaattattat tatctgttgt ttttacttta atgggttgaa tttaagtaag aaaggaacta
     8761 acagtgtgat attaaggtgc aatgttagac atataaaaca gtctttcacc tctctttggt
     8821 tatgtcttga attggtttgt ttcttcactt atctgtgtaa tcaagtttac tatgagtcta
     8881 tgatcaagta attatgcaat caagttaagt acagtatagg ctttttgtgt cgagggggta
     8941 ccgagtcgag gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9001 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9061 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcggg taccgagctc
     9121 gaattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     9181 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     9241 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     9301 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     9361 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     9421 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     9481 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     9541 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     9601 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     9661 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     9721 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     9781 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     9841 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     9901 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     9961 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
    10021 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
    10081 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
    10141 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
    10201 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
    10261 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
    10321 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
    10381 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
    10441 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
    10501 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
    10561 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
    10621 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
    10681 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10921 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11401 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11461 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11521 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
    11581 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
    11641 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
    11701 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    11761 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    11821 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
    11881 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
    11941 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
    12001 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
    12061 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
    12121 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
    12181 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
    12241 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
    12301 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
    12361 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
    12421 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
    12481 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
    12541 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
    12601 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
    12661 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
    12721 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
    12781 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
    12841 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    12901 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    12961 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    13021 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    13081 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    13141 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    13201 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    13261 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    13321 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    13381 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
    13441 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    13501 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    13561 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    13621 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    13681 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    13741 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    13801 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    13861 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    13921 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    13981 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    14041 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    14101 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    14161 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    14221 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    14281 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    14341 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    14401 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    14461 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    14521 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    14581 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    14641 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    14701 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    14761 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    14821 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    14881 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    14941 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    15001 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    15061 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    15121 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    15181 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    15241 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    15301 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    15361 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    15421 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    15481 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    15541 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    15601 gatcaaagta ctttaaagta cttt
//
