LOCUS       sequence-419133-        8370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human SYK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8370)
  TITLE     TREAT-AD Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDEST-GST-hSYK
FEATURES             Location/Qualifiers
     source          1..8370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             632..1285
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1243..1265
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     protein_bind    1313..1337
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3243..3267)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3299..3318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3300..3318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3329..3346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3329..3345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3338..3360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    3750..3884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3787..3806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3841..3860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4010..4465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4097..4116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4307..4328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4582..4939
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4582..4773
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4605..4625)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4790..4925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4990..5784
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5044..5063)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5654..5673
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5848..5896
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5937..5956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6056..6078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6202..6306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6307..6375,6376..7167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6525..6544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7338..7926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7827..7846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8080..8097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(8171..8275)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggac catggcccct atactaggtt attggaaaat
      661 taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga
      721 gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt
      781 ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc
      841 catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc
      901 agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc
      961 atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct
     1021 gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca
     1081 tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct
     1141 ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga
     1201 taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt
     1261 tggtggtggc gaccatcctc caaaatcgga tctggttccg cgttctagat caacaagttt
     1321 gtacaaaaaa gttggcacca tggccagcag cggcatggct gacagcgcca accacctgcc
     1381 cttctttttc ggcaacatca cccgggagga ggcagaagat tacctggtcc aggggggcat
     1441 gagtgatggg ctttatttgc tgcgccagag ccgcaactac ctgggtggct tcgccctgtc
     1501 cgtggcccac gggaggaagg cacaccacta caccatcgag cgggagctga atggcaccta
     1561 cgccatcgcc ggtggcagga cccatgccag ccccgccgac ctctgccact accactccca
     1621 ggagtctgat ggcctggtct gcctcctcaa gaagcccttc aaccggcccc aaggggtgca
     1681 gcccaagact gggccctttg aggatttgaa ggaaaacctc atcagggaat atgtgaagca
     1741 gacatggaac ctgcagggtc aggctctgga gcaggccatc atcagtcaga agcctcagct
     1801 ggagaagctg atcgctacca cagcccatga aaaaatgcct tggttccatg gaaaaatctc
     1861 tcgggaagaa tctgagcaaa ttgtcctgat aggatcaaag acaaatggaa agttcctgat
     1921 ccgagccaga gacaacaacg gctcctacgc cctgtgcctg ctgcacgaag ggaaggtgct
     1981 gcactatcgc atcgacaaag acaagacagg gaagctctcc atccccgagg gaaagaagtt
     2041 cgacacgctc tggcagctag tcgagcatta ttcttataaa gcagatggtt tgttaagagt
     2101 tcttactgtc ccatgtcaaa aaatcggcac acagggaaat gttaattttg gaggccgtcc
     2161 acaacttcca ggttcccatc ctgcgacttg gtcagcgggt ggaataatct caagaatcaa
     2221 atcatactcc ttcccaaagc ctggccacag aaagtcctcc cctgcccaag ggaaccggca
     2281 agagagtact gtgtcattca atccgtatga gccagaactt gcaccctggg ctgcagacaa
     2341 aggcccccag agagaagccc tacccatgga cacagaggtg tacgagagcc cctacgcgga
     2401 ccctgaggag atcaggccca aggaggttta cctggaccga aagctgctga cgctggaaga
     2461 caaagaactg ggctctggta attttggaac tgtgaaaaag ggctactacc aaatgaaaaa
     2521 agttgtgaaa accgtggctg tgaaaatact gaaaaacgag gccaatgacc ccgctcttaa
     2581 agatgagtta ttagcagaag caaatgtcat gcagcagctg gacaacccgt acatcgtgcg
     2641 catgatcggg atatgcgagg ccgagtcctg gatgctagtt atggagatgg cagaacttgg
     2701 tcccctcaat aagtatttgc agcagaacag acatgtcaag gataagaaca tcatagaact
     2761 ggttcatcag gtttccatgg gcatgaagta cttggaggag agcaattttg tgcacagaga
     2821 tctggctgca agaaatgtgt tgctagttac ccaacattat gccaagatca gtgatttcgg
     2881 actctccaaa gcactgcgtg ctgatgaaaa ctactacaag gcccagaccc atggaaagtg
     2941 gcctgtcaag tggtacgctc cggaatgcat caactactac aagttctcca gcaaaagcga
     3001 tgtctggagc tttggagtgt tgatgtggga agcattctcc tatgggcaga agccatatcg
     3061 agggatgaaa ggaagtgaag tcaccgctat gttagagaaa ggagagcgga tggggtgccc
     3121 tgcagggtgt ccaagagaga tgtacgatct catgaatctg tgctggacat acgatgtgga
     3181 aaacaggccc ggattcgcag cagtggaact gcggctgcgc aattactact atgacgtggt
     3241 gaacccaact ttcttgtaca aagtggttga tcgcgtgcat gcgacgtcat agctctctcc
     3301 ctatagtgag tcgtattata agctaggcac tggccgtcgt tttacaacgt cgtgactggg
     3361 aaaactgcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     3421 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     3481 gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag tatgatttat
     3541 gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag attcacagtc
     3601 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata
     3661 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3721 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3781 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3841 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgatcct gcattaatga
     3901 atcggccaac gcgcggggag aggcggtttg cgtattggct ggcgtaatag cgaagaggcc
     3961 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4021 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4081 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4141 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4201 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4261 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4321 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4381 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4441 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     4501 gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa cctctgaaag
     4561 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     4621 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4681 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4741 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4801 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4861 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4921 aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga acttaaggct
     4981 agagccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5041 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5101 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5161 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5221 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5281 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5341 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5401 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5461 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5521 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5581 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5641 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5701 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5761 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5821 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     5881 gttggttttt tgtgtgaatc gatagcgata aggatccgcg tatggtgcac tctcagtaca
     5941 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     6001 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6061 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     6121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     6181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6541 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6601 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6661 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6721 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6781 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6841 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6901 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7021 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7981 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8041 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8101 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8161 agagcttgca attcgcgctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8221 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8281 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     8341 aaaataggcg tattacgagg ccctttcacg
//
