LOCUS       sequence-419145-       19499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cas9 in the model legume Medicago truncatula, Gmubi 
            promoter (PGmubi), DsRed and Basta selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19499)
  TITLE     Christian Staehelin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 19499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pISV-CRISPR/Cas9PGmUbi
FEATURES             Location/Qualifiers
     source          1..19499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..50
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(297..318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     509..528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     653..675
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     667..684
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     668..684
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(697..897)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(932..1483)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1534..1713)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2192..2536
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2546..3223
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2726..2746)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3138..3161
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        3720..4065
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        5091..5137
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             5287..5307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5332..9432
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5332..9432
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6518..6526
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9433..9480
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    9844..9865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9880..9885,9886..9903,9904..9910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9918..9934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9976..9994
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9976..9993
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             10068..10370
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10249..10268
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      10512..10764
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10935..10959
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11424..12074
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(12262..12279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12513..12532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            12755..12864
                     /note="incP origin of transfer"
     CDS             12897..13268
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      13395..14106
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  14729..15496
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             15688..16482
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             16781..17929
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
       61 ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc
      121 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
      181 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa
      241 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag
      301 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      361 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
      421 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      481 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc
      541 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      601 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      661 cgacgttgta aaacgacggc cagtgaatta attcccatct tgaaagaaat atagtttaaa
      721 tatttattga taaaataaca agtcaggtat tatagtccaa gcaaaaacat aaatttattg
      781 atgcaagttt aaattcagaa atatttcaat aactgattat atcagctggt acattgccgt
      841 agatgaaaga ctgagtgcga tattatgtgt aatacataaa ttgatgatat agctagctta
      901 gctcatcggg ggatcgatcc tctaggggtc atcagatctc ggtgacgggc aggaccggac
      961 ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
     1021 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
     1081 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
     1141 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
     1201 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc
     1261 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
     1321 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
     1381 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
     1441 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat ccccctagag
     1501 tcgagatctg gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg
     1561 aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac
     1621 ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc
     1681 cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg
     1741 tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga
     1801 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     1861 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     1921 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     1981 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     2041 ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt
     2101 gcggcagcgt gaagcttaac atggtggagc acgacactct cgtctactcc aagaatatca
     2161 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     2221 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     2281 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     2341 cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag
     2401 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     2461 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     2521 ttcatttgga gaggacctcg agacaatggc ctcctccgag aacgtcatca ccgagttcat
     2581 gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga
     2641 gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg
     2701 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
     2761 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
     2821 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc
     2881 cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
     2941 cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     3001 ccgcgacggc gtgctgaagg gcgaaacaca caaggccctg aagctgaagg acggcggcca
     3061 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
     3121 ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     3181 gcagtacgag cgcaccgagg gccgccacca cctgttcctg tgatctagag tccgcaaaaa
     3241 tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt
     3301 agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     3361 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     3421 taaaaccaaa atcaagcttg catgcctgca ggtccgattg agacttttca acaaagggta
     3481 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
     3541 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
     3601 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     3661 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tggtccgatt
     3721 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     3781 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     3841 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     3901 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     3961 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     4021 gacccttcct ctatataagg aagttcattt catttggaga ggagatccta gagcggccgc
     4081 ggatccgtta acatcgattc tagagaattc ccttaattaa gggcccaata taacaacgac
     4141 gtcgtaacag ataaagcgaa gcttgaaggt gcatgtgact ccgtcaagat tacgaaaccg
     4201 ccaactacca cgcaaattgc aattctcaat ttcctagaag gactctccga aaatgcatcc
     4261 aataccaaat attacccgtg tcataggcac caagtgacac catacatgaa cacgcgtcac
     4321 aatatgactg gagaagggtt ccacacctta tgctataaaa cgccccacac ccctcctcct
     4381 tccttcgcag ttcaattcca atatattcca ttctctctgt gtatttccct acctctccct
     4441 tcaaggttag tcgatttctt ctgtttttct tcttcgttct ttccatgaat tgtgtatgtt
     4501 ctttgatcaa tacgatgttg atttgattgt gttttgtttg gtttcatcga tcttcaattt
     4561 tcataatcag attcagcttt tattatcttt acaacaacgt ccttaatttg atgattcttt
     4621 aatcgtagat ttgctctaat tagagctttt tcatgtcaga tccctttaca acaagcctta
     4681 attgttgatt cattaatcgt agattagggc ttttttcatt gattacttca gatccgttaa
     4741 acgtaaccat agatcagggc tttttcatga attacttcag atccgttaaa caacagcctt
     4801 attttttata cttctgtggt ttttcaagaa attgttcaga tccgttgaca aaaagcctta
     4861 ttcgttgatt ctatatcgtt tttcgagaga tattgctcag atctgttagc aactgccttg
     4921 tttgttgatt ctattgccgt ggattagggt tttttttcac gagattgctt cagatccgta
     4981 cttaagatta cgtaatggat tttgattctg atttatctgt gattgttgac tcgacagcct
     5041 taattaatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     5101 tcctctatat aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca
     5161 tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa
     5221 tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc
     5281 atggctccta agaagaagcg gaaggttggt attcacgggg tgcctgcggc tgacaagaag
     5341 tactccatcg gcctcgacat cggcaccaac agcgtcggct gggcggtgat caccgacgag
     5401 tacaaggtcc cgtccaagaa gttcaaggtc ctgggcaaca ccgaccgcca ctccatcaag
     5461 aagaacctca tcggcgccct cctcttcgac tccggcgaga cggcggaggc gacccgcctc
     5521 aagcgcaccg cccgccgccg ctacacccgc cgcaagaacc gcatctgcta cctccaggag
     5581 atcttctcca acgagatggc gaaggtcgac gactccttct tccaccgcct cgaggagtcc
     5641 ttcctcgtgg aggaggacaa gaagcacgag cgccacccca tcttcggcaa catcgtcgac
     5701 gaggtcgcct accacgagaa gtaccccact atctaccacc ttcgtaagaa gcttgttgac
     5761 tctactgata aggctgatct tcgtctcatc taccttgctc tcgctcacat gatcaagttc
     5821 cgtggtcact tccttatcga gggtgacctt aaccctgata actccgacgt ggacaagctc
     5881 ttcatccagc tcgtccagac ctacaaccag ctcttcgagg agaaccctat caacgcttcc
     5941 ggtgtcgacg ctaaggcgat cctttccgct aggctctcca agtccaggcg tctcgagaac
     6001 ctcatcgccc agctccctgg tgagaagaag aacggtcttt tcggtaacct catcgctctc
     6061 tccctcggtc tgacccctaa cttcaagtcc aacttcgacc tcgctgagga cgctaagctt
     6121 cagctctcca aggataccta cgacgatgat ctcgacaacc tcctcgctca gattggagat
     6181 cagtacgctg atctcttcct tgctgctaag aacctctccg atgctatcct cctttcggat
     6241 atccttaggg ttaacactga gatcactaag gctcctcttt ctgcttccat gatcaagcgc
     6301 tacgacgagc accaccagga cctcaccctc ctcaaggctc ttgttcgtca gcagctcccc
     6361 gagaagtaca aggagatctt cttcgaccag tccaagaacg gctacgccgg ttacattgac
     6421 ggtggagcta gccaggagga gttctacaag ttcatcaagc caatccttga gaagatggat
     6481 ggtactgagg agcttctcgt taagcttaac cgtgaggacc tccttaggaa gcagaggact
     6541 ttcgataacg gctctatccc tcaccagatc caccttggtg agcttcacgc catccttcgt
     6601 aggcaggagg acttctaccc tttcctcaag gacaaccgtg agaagatcga gaagatcctt
     6661 actttccgta ttccttacta cgttggtcct cttgctcgtg gtaactcccg tttcgcttgg
     6721 atgactagga agtccgagga gactatcacc ccttggaact tcgaggaggt tgttgacaag
     6781 ggtgcttccg cccagtcctt catcgagcgc atgaccaact tcgacaagaa cctccccaac
     6841 gagaaggtcc tccccaagca ctccctcctc tacgagtact tcacggtcta caacgagctc
     6901 accaaggtca agtacgtcac cgagggtatg cgcaagcctg ccttcctctc cggcgagcag
     6961 aagaaggcta tcgttgacct cctcttcaag accaaccgca aggtcaccgt caagcagctc
     7021 aaggaggact acttcaagaa gatcgagtgc ttcgactccg tcgagatcag cggcgttgag
     7081 gaccgtttca acgcttctct cggtacctac cacgatctcc tcaagatcat caaggacaag
     7141 gacttcctcg acaacgagga gaacgaggac atcctcgagg acatcgtcct cactcttact
     7201 ctcttcgagg atagggagat gatcgaggag aggctcaaga cttacgctca tctcttcgat
     7261 gacaaggtta tgaagcagct caagcgtcgc cgttacaccg gttggggtag gctctcccgc
     7321 aagctcatca acggtatcag ggataagcag agcggcaaga ctatcctcga cttcctcaag
     7381 tctgatggtt tcgctaacag gaacttcatg cagctcatcc acgatgactc tcttaccttc
     7441 aaggaggata ttcagaaggc tcaggtgtcc ggtcagggcg actctctcca cgagcacatt
     7501 gctaaccttg ctggttcccc tgctatcaag aagggcatcc ttcagactgt taaggttgtc
     7561 gatgagcttg tcaaggttat gggtcgtcac aagcctgaga acatcgtcat cgagatggct
     7621 cgtgagaacc agactaccca gaagggtcag aagaactcga gggagcgcat gaagaggatt
     7681 gaggagggta tcaaggagct tggttctcag atccttaagg agcaccctgt cgagaacacc
     7741 cagctccaga acgagaagct ctacctctac tacctccaga acggtaggga tatgtacgtt
     7801 gaccaggagc tcgacatcaa caggctttct gactacgacg tcgaccacat tgttcctcag
     7861 tctttcctta aggatgactc catcgacaac aaggtcctca cgaggtccga caagaacagg
     7921 ggtaagtcgg acaacgtccc ttccgaggag gttgtcaaga agatgaagaa ctactggagg
     7981 cagcttctca acgctaagct cattacccag aggaagttcg acaacctcac gaaggctgag
     8041 aggggtggcc tttccgagct tgacaaggct ggtttcatca agaggcagct tgttgagacg
     8101 aggcagatta ccaagcacgt tgctcagatc ctcgattcta ggatgaacac caagtacgac
     8161 gagaacgaca agctcatccg cgaggtcaag gtgatcaccc tcaagtccaa gctcgtctcc
     8221 gacttccgca aggacttcca gttctacaag gtccgcgaga tcaacaacta ccaccacgct
     8281 cacgatgctt accttaacgc tgtcgttggt accgctctta tcaagaagta ccctaagctt
     8341 gagtccgagt tcgtctacgg tgactacaag gtctacgacg ttcgtaagat gatcgccaag
     8401 tccgagcagg agatcggcaa ggccaccgcc aagtacttct tctactccaa catcatgaac
     8461 ttcttcaaga ccgagatcac cctcgccaac ggcgagatcc gcaagcgccc tcttatcgag
     8521 acgaacggtg agactggtga gatcgtttgg gacaagggtc gcgacttcgc tactgttcgc
     8581 aaggtccttt ctatgcctca ggttaacatc gtcaagaaga ccgaggtcca gaccggtggc
     8641 ttctccaagg agtctatcct tccaaagaga aactcggaca agctcatcgc taggaagaag
     8701 gattgggacc ctaagaagta cggtggtttc gactccccta ctgtcgccta ctccgtcctc
     8761 gtggtcgcca aggtggagaa gggtaagtcg aagaagctca agtccgtcaa ggagctcctc
     8821 ggcatcacca tcatggagcg ctcctccttc gagaagaacc cgatcgactt cctcgaggcc
     8881 aagggctaca aggaggtcaa gaaggacctc atcatcaagc tccccaagta ctctcttttc
     8941 gagctcgaga acggtcgtaa gaggatgctg gcttccgctg gtgagctcca gaagggtaac
     9001 gagcttgctc ttccttccaa gtacgtgaac ttcctctacc tcgcctccca ctacgagaag
     9061 ctcaagggtt cccctgagga taacgagcag aagcagctct tcgtggagca gcacaagcac
     9121 tacctcgacg agatcatcga gcagatctcc gagttctcca agcgcgtcat cctcgctgac
     9181 gctaacctcg acaaggtcct ctccgcctac aacaagcacc gcgacaagcc catccgcgag
     9241 caggccgaga acatcatcca cctcttcacg ctcacgaacc tcggcgcccc tgctgctttc
     9301 aagtacttcg acaccaccat cgacaggaag cgttacacgt ccaccaagga ggttctcgac
     9361 gctactctca tccaccagtc catcaccggt ctttacgaga ctcgtatcga cctttcccag
     9421 cttggtggtg ataagcgtcc tgctgccacc aaaaaggccg gacaggctaa gaaaaagaag
     9481 taggatccac ctgatctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc
     9541 tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt
     9601 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     9661 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga cgcggtcgtg
     9721 ctccacatgt tgaccggtaa ggcgcgccgt agtgctcgag agacctctga agtggccgat
     9781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaagaaaca gctatgacca
     9961 tgattacgcc aagctattta ggtgacacta tagaatactc aagctatgca tcaagctcaa
    10021 tgggtctagt ctgtagatac ccatcacact ggcgaccgct cgaacatcag tttaaggttt
    10081 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
    10141 acacgccggg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
    10201 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
    10261 ccgatatggc cagtgtgcct gtctccgtta tcggggaaga agtggctgat ctcagccacc
    10321 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggcc
    10381 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg agcgtgggtc
    10441 tcgcggtatc attggcgcgc ctctcgagct agaattcgtc gaccccgggc tcgaggagct
    10501 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
    10561 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
    10621 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
    10681 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
    10741 cggtgtcatc tatgttacta gatcgggaat taattcagat cggctgagtg gctccttcaa
    10801 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
    10861 ataacgtgac tcccttaatt ctccgctcat gatcagattg tcgtttcccg ccttcagttt
    10921 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
    10981 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
    11041 gcatgccaac cacagggttc cccagatctg gcgccggcca gcgagacgag caagattggc
    11101 cgccgcccga aacgatccga cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac
    11161 gcatacagcg ccagcagaat gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg
    11221 cgcaggaggc ccggcagcac cggcataatc aggccgatgc cgacagcgtc gagcgcgaca
    11281 gtgctcagaa ttacgatcag gggtatgttg ggtttcacgt ctggcctccg gaccagcctc
    11341 cgctggtccg attgaacgcg cggattcttt atcactgata agttggtgga catattatgt
    11401 ttatcagtga taaagtgtca agcatgacaa agttgcagcc gaatacagtg atccgtgccg
    11461 ccctggacct gttgaacgag gtcggcgtag acggtctgac gacacgcaaa ctggcggaac
    11521 ggttgggggt tcagcagccg gcgctttact ggcacttcag gaacaagcgg gcgctgctcg
    11581 acgcactggc cgaagccatg ctggcggaga atcatacgca ttcggtgccg agagccgacg
    11641 acgactggcg ctcatttctg atcgggaatg cccgcagctt caggcaggcg ctgctcgcct
    11701 accgcgatgg cgcgcgcatc catgccggca cgcgaccggg cgcaccgcag atggaaacgg
    11761 ccgacgcgca gcttcgcttc ctctgcgagg cgggtttttc ggccggggac gccgtcaatg
    11821 cgctgatgac aatcagctac ttcactgttg gggccgtgct tgaggagcag gccggcgaca
    11881 gcgatgccgg cgagcgcggc ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg
    11941 ccgcgataga cgccttcgac gaagccggtc cggacgcagc gttcgagcag ggactcgcgg
    12001 tgattgtcga tggattggcg aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa
    12061 agggtgacga ttgatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat
    12121 gtagacaaca tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg
    12181 ctttttcatg ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc
    12241 cttctggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12601 cccttcggga agcgtggcgc ttttccgctg cataaccctg cttcggggtc attatagcga
    12661 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
    12721 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
    12781 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
    12841 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
    12901 ctgatgaaac caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag
    12961 acgaacgaag agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc
    13021 tgctggccgt cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg
    13081 agctggcccg catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca
    13141 ccgacgaccc gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga
    13201 tcgaagagaa gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag
    13261 agccatgact tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc
    13321 cccatgcgct ccatcaagaa gagcgacttc gcggagctgg tgaagtacat caccgacgag
    13381 caaggcaaga ccgagcgcct ttccgacgct caccgggctg gttgccctcg ccgctgggct
    13441 ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca
    13501 ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg
    13561 ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca
    13621 ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg
    13681 attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta
    13741 ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg
    13801 gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggcag
    13861 aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta
    13921 acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc
    13981 gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctaacg
    14041 cgggcctccc atccccccag gggctgcgcc cctcggccgc gaacggcctc accccaaaaa
    14101 tggcagcgct ggcagtcctt gccattgccg ggatcggggc agtaacggga tgggcgatca
    14161 gcccgagcgc gacgcccgga agcattgacg tgccgcaggt gctggcatcg acattcagcg
    14221 accaggtgcc gggcagtgag ggcggcggcc tgggtggcgg cctgcccttc acttcggccg
    14281 tcggggcatt cacggacttc atggcggggc cggcaatttt taccttgggc attcttggca
    14341 tagtggtcgc gggtgccgtg ctcgtgttcg ggggtgcgat aaacccagcg aaccatttga
    14401 ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact
    14461 ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc
    14521 agattgcctt gaatatattg acaatactga taagataata tatcttttat atagaagata
    14581 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
    14641 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
    14701 ttatagcttg taaattctat cataattggg taatgactcc aacttattga tagtgtttta
    14761 tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag aacgacagcg
    14821 acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct
    14881 gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc
    14941 agccatccgt catccatatc accacgtcaa agggtgacag caggctcata agacgcccca
    15001 gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat
    15061 acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac tgttcgtcca
    15121 tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc gactgcggcc
    15181 tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc gggctgttgc
    15241 ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac cgggttgaga
    15301 agcggtgtaa gtgaactgca gttgccatgt tttacggcag tgagagcaga gatagcgctg
    15361 atgtccggcg gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca
    15421 gctgatagaa acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta
    15481 agttggcagc atcacccata attgtggttt caaaatcggc tccgtcgata ctatgttata
    15541 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
    15601 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
    15661 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
    15721 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
    15781 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
    15841 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
    15901 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
    15961 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
    16021 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
    16081 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
    16141 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
    16201 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
    16261 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
    16321 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
    16381 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
    16441 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tgtggcgcaa
    16501 cgatgccggc gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca
    16561 ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat
    16621 tcggaatacc aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga
    16681 taaggtggat tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat
    16741 tgccccggcg tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg
    16801 gaaggcatac aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat
    16861 cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt
    16921 ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc
    16981 cgcgccatcg gccgccgtgg agcgttcgcg tcgtctcgaa caggaggcgg caggtttggc
    17041 gaagtcgatg accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg
    17101 cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa
    17161 gcagcagatc aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat
    17221 gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat
    17281 cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac
    17341 ctacaccggc gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga
    17401 gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca
    17461 ggacctgggc tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg
    17521 cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc
    17581 gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct
    17641 gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga
    17701 gaagtaccgc aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg
    17761 ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg
    17821 cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct
    17881 ggtggaacac gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg
    17941 gtcagttccg gctgggggtt cagcagccag cgctttactg gcatttcagg aacaagcggg
    18001 cactgctcga cgcacttgct tcgctcagta tcgctcggga cgcacggcgc gctctacgaa
    18061 ctgccgataa acagaggatt aaaattgaca attgtgatta aggctcagat tcgacggctt
    18121 ggagcggccg acgtgcagga tttccgcgag atccgattgt cggccctgaa gaaagctcca
    18181 gagatgttcg ggtccgttta cgagcacgag gagaaaaagc ccatggaggc gttcgctgaa
    18241 cggttgcgag atgccgtggc attcggcgcc tacatcgacg gcgagatcat tgggctgtcg
    18301 gtcttcaaac aggaggacgg ccccaaggac gctcacaagg cgcatctgtc cggcgttttc
    18361 gtggagcccg aacagcgagg ccgaggggtc gccggtatgc tgctgcgggc gttgccggcg
    18421 ggtttattgc tcgtgatgat cgtccgacag attccaacgg gaatctggtg gatgcgcatc
    18481 ttcatcctcg gcgcacttaa tatttcgcta ttctggagct tgttgtttat ttcggtctac
    18541 cgcctgccgg gcggggtcgc ggcgacggta ggcgctgtgc agccgctgat ggtcgtgttc
    18601 atctctgccg ctctgctagg tagcccgata cgattgatgg cggtcctggg ggctatttgc
    18661 ggaactgcgg gcgtggcgct gttggtgttg acaccaaacg cagcgctaga tcctgtcggc
    18721 gtcgcagcgg gcctggcggg ggcggtttcc atggcgttcg gaaccgtgct gacccgcaag
    18781 tggcaacctc ccgtgcctct gctcaccttt accgcctggc aactggcggc cggaggactt
    18841 ctgctcgttc cagtagcttt agtgtttgat ccgccaatcc cgatgcctac aggaaccaat
    18901 gttctcggcc tggcgtggct cggcctgatc ggagcgggtt taacctactt cctttggttc
    18961 cgggggatct cgcgactcga acctacagtt gtttccttac tgggctttct cagccccaga
    19021 tctggggtcg atcagccggg gatgcatcag gccgacagtc ggaacttcgg gtccccgacc
    19081 tgtaccattc ggtgagcaat ggatagggga gttgatatcg tcaacgttca cttctaaaga
    19141 aatagcgcca ctcagcttcc tcagcggctt tatccagcga tttcctatta tgtcggcata
    19201 gttctcaaga tcgacagcct gtcacggtta agcgagaaat gaataagaag gctgataatt
    19261 cggatctctg cgagggagat gatatttgat cacaggcagc aacgctctgt catcgttaca
    19321 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
    19381 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
    19441 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgcc
//
