LOCUS       sequence-419146-       12928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging plasmid for v3 PE-eVLP production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 12928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: gag-PE
FEATURES             Location/Qualifiers
     source          1..12928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..420
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     350..372
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     388..404
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             649..675
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             688..714
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             727..753
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1831..1851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1852..5952
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6010..6030
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8113..8133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8143..8169
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(8175..8194)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8240..8295
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8294..8313)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8653..8669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8653..8669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8666..8688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8677..8693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8701..8707,8708..8725,8726..8731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8746..8767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8884..8901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9055..9643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9135..9154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9814..10605,10606..10674))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10437..10456
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10675..10779)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10805..11260)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10942..10963)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11154..11173
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11387..11409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11401..11418
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11402..11418
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11428..11447
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11428..11446
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        11567..11946
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11947..12150
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12101..12121
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12147..12171
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          12262..12834
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
       61 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
      121 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
      181 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
      241 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
      301 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
      361 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
      421 aaacctaaac ctcaagttct ttctgacagt ggggggccgc tcatcgacct acttacagaa
      481 gaccccccgc cttataggga cccaagacca cccccttccg acagggacgg aaatggtgga
      541 gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa tggcatctcg cctacgtggg
      601 agacgggagc cccctgtggc cgactccact tctggcggct cacttcaact gcctccactt
      661 gaaagactga cactgggatc attacaatta cctcctttag aacgattaac actcggttca
      721 ctacagcttc cgcctcttga gagattgaca ttaagtggtg gatctacctc gcaggcattc
      781 cccctccgcg caggaggaaa cggacagctt caatactggc cgttctcctc ttctgacctt
      841 tacaactgga aaaataataa cccttctttt tctgaagatc caggtaaact gacagctctg
      901 atcgagtctg tcctcatcac ccatcagccc acctgggacg actgtcagca gctgttgggg
      961 actctgctga ccggagaaga aaaacaacgg gtgctcttag aggctagaaa ggcggtgcgg
     1021 ggcgatgatg ggcgccccac tcaactgccc aatgaagtcg atgccgcttt tcccctcgag
     1081 cgcccagact gggattacac cacccaggca ggtaggaacc acctagtcca ctatcgccag
     1141 ttgctcctag cgggtctcca aaacgcgggc agaagcccca ccaatttggc caaggtaaaa
     1201 ggaataacac aagggcccaa tgagtctccc tcggccttcc tagagagact taaggaagcc
     1261 tatcgcaggt acactcctta tgaccctgag gacccagggc aagaaactaa tgtgtctatg
     1321 tctttcattt ggcagtctgc cccagacatt gggagaaagt tagagaggtt agaagattta
     1381 aaaaacaaga cgcttggaga tttggttaga gaggcagaaa agatctttaa taaacgagaa
     1441 accccggaag aaagagagga acgtatcagg agagaaacag aggaaaaaga agaacgccgt
     1501 aggacagagg atgagcagaa agagaaagaa agagatcgta ggagacatag agagatgagc
     1561 aagctattgg ccactgtcgt tagtggacag aaacaggata gacagggagg agaacgaagg
     1621 aggtcccaac tcgatcgcga ccagtgtgcc tactgcaaag aaaaggggca ctgggctaaa
     1681 gattgtccca agaaaccacg aggacctcgg ggaccaagac cccagacctc cctcctgacc
     1741 ctagatgacg gcggatccac gtccacgctg ctaatggaga actcgtctgg aggttctaaa
     1801 cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt cgacaagaag
     1861 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     1921 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     1981 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     2041 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     2101 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     2161 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     2221 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     2281 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     2341 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     2401 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     2461 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagaaa gctggaaaat
     2521 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     2581 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     2641 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     2701 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     2761 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     2821 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     2881 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     2941 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     3001 ggcaccgagg aactgctcgt gaagctgaag agagaggacc tgctgcggaa gcagcggacc
     3061 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     3121 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     3181 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     3241 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     3301 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     3361 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     3421 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     3481 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     3541 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     3601 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     3661 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     3721 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     3781 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     3841 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     3901 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     3961 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     4021 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     4081 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     4141 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     4201 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     4261 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     4321 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag
     4381 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     4441 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     4501 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     4561 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     4621 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     4681 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     4741 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     4801 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     4861 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     4921 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     4981 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     5041 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     5101 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     5161 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
     5221 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
     5281 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     5341 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     5401 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     5461 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
     5521 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     5581 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     5641 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     5701 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     5761 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
     5821 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     5881 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     5941 ctgggaggtg actccggcgg aagctctggt ggcagcaagc ggaccgccga cggctctgaa
     6001 ttcgagagcc ctaagaagaa aagaaaggtg agcggaggct ctagcggcgg aagcaccctg
     6061 aacattgaag acgagtatag actgcatgaa acaagcaagg aacccgacgt gtccctgggc
     6121 tccacctggc tgtccgactt tccccaggcc tgggccgaga caggaggaat gggcctggcc
     6181 gtgcggcagg cacccctgat catccctctg aaggccacct ctacacccgt gagcatcaag
     6241 cagtacccta tgtctcagga ggccagactg ggcatcaagc ctcacatcca gaggctgctg
     6301 gaccagggca tcctggtgcc atgccagagc ccctggaaca caccactgct gcccgtgaag
     6361 aagccaggca ccaatgacta tagacccgtg caggatctga gagaggtgaa caagagggtg
     6421 gaggatatcc accccaccgt gcccaaccct tacaatctgc tgtccggcct gcccccttct
     6481 caccagtggt atacagtgct ggacctgaag gatgccttct tttgtctgag actgcaccct
     6541 accagccagc cactgttcgc ctttgagtgg agggaccctg agatgggcat ctctggccag
     6601 ctgacctgga cacgcctgcc tcagggcttc aagaatagcc caacactgtt taacgaggcc
     6661 ctgcaccgcg acctggcaga tttccggatc cagcacccag atctgatcct gctgcagtac
     6721 gtggacgatc tgctgctggc cgccaccagc gagctggatt gccagcaggg aacacgcgcc
     6781 ctgctgcaga ccctgggaaa cctgggatat agggcatccg ccaagaaggc ccagatctgt
     6841 cagaagcagg tgaagtacct gggctatctg ctgaaggagg gccagagatg gctgacagag
     6901 gccaggaagg agacagtgat gggccagcca acacccaaga ccccaagaca gctgagggag
     6961 ttcctgggca aagcaggatt ttgcaggctg ttcatcccag gattcgcaga gatggcagca
     7021 cctctgtacc cactgaccaa gccgggcacc ctgtttaatt ggggccctga ccagcagaag
     7081 gcctatcagg agatcaagca ggccctgctg acagcaccag ccctgggcct gccagacctg
     7141 accaagcctt tcgagctgtt tgtggatgag aagcagggct acgccaaggg cgtgctgacc
     7201 cagaagctgg gaccatggag acggcccgtg gcctatctgt ccaagaagct ggacccagtg
     7261 gcagcaggat ggccaccatg cctgaggatg gtggcagcaa tcgccgtgct gacaaaggat
     7321 gccggcaagc tgaccatggg acagccactg gtcatcctgg caccacacgc agtggaggcc
     7381 ctggtgaagc agcctccaga tcgctggctg tctaacgccc ggatgacaca ctaccaggcc
     7441 ctgctgctgg acaccgatcg cgtgcagttt ggccctgtgg tggccctgaa tccagccacc
     7501 ctgctgcctc tgccagagga gggcctgcag cacaactgtc tggacatcct ggcagaggca
     7561 cacggaacaa ggccagacct gaccgatcag cccctgcctg acgccgatca cacatggtat
     7621 accgatggaa gctccctgct gcaggagggc cagaggaagg caggagcagc agtgaccaca
     7681 gagacagaag tgatctgggc caaggccctg ccagcaggca catccgccca gcgggccgag
     7741 ctgatcgccc tgacccaggc cctgaagatg gccgagggca agaagctgaa cgtgtacaca
     7801 gactccagat atgccttcgc caccgcacac atccacggag agatctacag gcgccggggc
     7861 tggctgacct ctgagggcaa ggagatcaag aacaaggatg agatcctggc cctgctgaag
     7921 gccctgtttc tgcccaagcg gctgagcatc atccactgtc ctggacacca gaagggacac
     7981 tccgccgagg caaggggcaa tcggatggcc gaccaggccg ccagaaaggc tgctattact
     8041 gaaactcccg acacttccac tctgctgtct ggcggctcaa aaagaaccgc cgacggcagc
     8101 gaattcgagt ctcccaagaa gaagaggaaa gtcggctctg gccctgccgc taagagagtg
     8161 aagctggact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     8221 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     8281 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     8341 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     8401 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     8461 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     8521 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     8581 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacgg atcggccgca
     8641 attcgtaacc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8701 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8761 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9241 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9481 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9721 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9781 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9841 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9901 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9961 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10021 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10081 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10141 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10201 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10261 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10321 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10381 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10441 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10501 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10561 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10621 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10681 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10741 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10801 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
    10861 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
    10921 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
    10981 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
    11041 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
    11101 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
    11161 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
    11221 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
    11281 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    11341 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    11401 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc
    11461 caccgcggtg gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat
    11521 aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg
    11581 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    11641 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    11701 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    11761 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    11821 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    11881 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    11941 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
    12001 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
    12061 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
    12121 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
    12181 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggtcg
    12241 accgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
    12301 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
    12361 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
    12421 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
    12481 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
    12541 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
    12601 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
    12661 tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg
    12721 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
    12781 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg
    12841 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcct cgagggccgg
    12901 atccgaattc ctgtatttgt ctgaaaac
//
