LOCUS       sequence-419148-       11443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging plasmid for v3b PE-eVLP production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: P4-PE
FEATURES             Location/Qualifiers
     source          1..11443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             133..159
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             172..198
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             211..237
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             289..312
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             346..366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             367..4467
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4525..4545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6628..6648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6658..6684
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(6690..6709)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6755..6810
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6809..6828)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7168..7184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7168..7184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7181..7203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7192..7208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7216..7222,7223..7240,7241..7246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7261..7282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7399..7416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7570..8158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7650..7669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8329..9120,9121..9189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8952..8971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9190..9294)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9320..9775)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9457..9478)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9669..9688
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9902..9924
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9916..9933
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9917..9933
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9943..9962
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9943..9961
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        10082..10461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10462..10665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10616..10636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10662..10686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          10777..11349
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 atgtctcccg aagataaaat agctcaactc aaacagaaaa ttcaggcttt gaaacaagaa
       61 aaccagcagt tggaggaaga aaatgcggcc ctggagtatg gcagtggcgg tagttctggc
      121 ggctcacttc aactgcctcc acttgaaaga ctgacactgg gatcattaca attacctcct
      181 ttagaacgat taacactcgg ttcactacag cttccgcctc ttgagagatt gacattaagt
      241 ggtggatcta cgtccacgct gctaatggag aactcgtctg gaggttctga ttacaaagac
      301 gatgacgata agaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg
      361 aaagtcgaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
      421 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
      481 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
      541 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
      601 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
      661 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttt
      721 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
      781 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
      841 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
      901 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
      961 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     1021 agaaagctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
     1081 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     1141 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     1201 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     1261 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     1321 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     1381 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     1441 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
     1501 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaagagaga ggacctgctg
     1561 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     1621 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     1681 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     1741 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     1801 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat
     1861 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     1921 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     1981 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     2041 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     2101 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     2161 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     2221 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     2281 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     2341 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     2401 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     2461 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     2521 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     2581 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     2641 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     2701 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     2761 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     2821 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     2881 gctatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga
     2941 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     3001 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat
     3061 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     3121 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     3181 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag
     3241 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     3301 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     3361 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     3421 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     3481 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     3541 cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat
     3601 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag
     3661 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg
     3721 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
     3781 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     3841 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     3901 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     3961 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
     4021 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     4081 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     4141 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     4201 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat
     4261 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga
     4321 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
     4381 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     4441 atcgacctgt ctcagctggg aggtgactcc ggcggaagct ctggtggcag caagcggacc
     4501 gccgacggct ctgaattcga gagccctaag aagaaaagaa aggtgagcgg aggctctagc
     4561 ggcggaagca ccctgaacat tgaagacgag tatagactgc atgaaacaag caaggaaccc
     4621 gacgtgtccc tgggctccac ctggctgtcc gactttcccc aggcctgggc cgagacagga
     4681 ggaatgggcc tggccgtgcg gcaggcaccc ctgatcatcc ctctgaaggc cacctctaca
     4741 cccgtgagca tcaagcagta ccctatgtct caggaggcca gactgggcat caagcctcac
     4801 atccagaggc tgctggacca gggcatcctg gtgccatgcc agagcccctg gaacacacca
     4861 ctgctgcccg tgaagaagcc aggcaccaat gactatagac ccgtgcagga tctgagagag
     4921 gtgaacaaga gggtggagga tatccacccc accgtgccca acccttacaa tctgctgtcc
     4981 ggcctgcccc cttctcacca gtggtataca gtgctggacc tgaaggatgc cttcttttgt
     5041 ctgagactgc accctaccag ccagccactg ttcgcctttg agtggaggga ccctgagatg
     5101 ggcatctctg gccagctgac ctggacacgc ctgcctcagg gcttcaagaa tagcccaaca
     5161 ctgtttaacg aggccctgca ccgcgacctg gcagatttcc ggatccagca cccagatctg
     5221 atcctgctgc agtacgtgga cgatctgctg ctggccgcca ccagcgagct ggattgccag
     5281 cagggaacac gcgccctgct gcagaccctg ggaaacctgg gatatagggc atccgccaag
     5341 aaggcccaga tctgtcagaa gcaggtgaag tacctgggct atctgctgaa ggagggccag
     5401 agatggctga cagaggccag gaaggagaca gtgatgggcc agccaacacc caagacccca
     5461 agacagctga gggagttcct gggcaaagca ggattttgca ggctgttcat cccaggattc
     5521 gcagagatgg cagcacctct gtacccactg accaagccgg gcaccctgtt taattggggc
     5581 cctgaccagc agaaggccta tcaggagatc aagcaggccc tgctgacagc accagccctg
     5641 ggcctgccag acctgaccaa gcctttcgag ctgtttgtgg atgagaagca gggctacgcc
     5701 aagggcgtgc tgacccagaa gctgggacca tggagacggc ccgtggccta tctgtccaag
     5761 aagctggacc cagtggcagc aggatggcca ccatgcctga ggatggtggc agcaatcgcc
     5821 gtgctgacaa aggatgccgg caagctgacc atgggacagc cactggtcat cctggcacca
     5881 cacgcagtgg aggccctggt gaagcagcct ccagatcgct ggctgtctaa cgcccggatg
     5941 acacactacc aggccctgct gctggacacc gatcgcgtgc agtttggccc tgtggtggcc
     6001 ctgaatccag ccaccctgct gcctctgcca gaggagggcc tgcagcacaa ctgtctggac
     6061 atcctggcag aggcacacgg aacaaggcca gacctgaccg atcagcccct gcctgacgcc
     6121 gatcacacat ggtataccga tggaagctcc ctgctgcagg agggccagag gaaggcagga
     6181 gcagcagtga ccacagagac agaagtgatc tgggccaagg ccctgccagc aggcacatcc
     6241 gcccagcggg ccgagctgat cgccctgacc caggccctga agatggccga gggcaagaag
     6301 ctgaacgtgt acacagactc cagatatgcc ttcgccaccg cacacatcca cggagagatc
     6361 tacaggcgcc ggggctggct gacctctgag ggcaaggaga tcaagaacaa ggatgagatc
     6421 ctggccctgc tgaaggccct gtttctgccc aagcggctga gcatcatcca ctgtcctgga
     6481 caccagaagg gacactccgc cgaggcaagg ggcaatcgga tggccgacca ggccgccaga
     6541 aaggctgcta ttactgaaac tcccgacact tccactctgc tgtctggcgg ctcaaaaaga
     6601 accgccgacg gcagcgaatt cgagtctccc aagaagaaga ggaaagtcgg ctctggccct
     6661 gccgctaaga gagtgaagct ggactgagat ctttttccct ctgccaaaaa ttatggggac
     6721 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     6781 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     6841 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     6901 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     6961 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     7021 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     7081 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     7141 gacggatcgg ccgcaattcg taaccatgtc atagctgttt cctgtgtgaa attgttatcc
     7201 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7261 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7381 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7441 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7501 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7561 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7621 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7681 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7741 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7801 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7861 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7921 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7981 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8041 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8101 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8161 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8221 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8281 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8341 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8401 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8461 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8521 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8581 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8641 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8701 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8761 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8821 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8881 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8941 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9001 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9061 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9301 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     9361 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     9421 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     9481 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     9541 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     9601 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     9661 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     9721 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     9781 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9841 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9901 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     9961 ggcgaattgg agctccaccg cggtggcggc cgctctagag agcttggccc attgcatacg
    10021 ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt accgccatgt
    10081 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
    10141 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    10201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    10261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
    10501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
    10561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
    10621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
    10681 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
    10741 tccagcctcc ggtcgaccga tcctgagaac ttcagggtga gtttggggac ccttgattgt
    10801 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
    10861 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
    10921 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
    10981 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
    11041 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
    11101 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
    11161 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacacc
    11221 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
    11281 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttctct
    11341 ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa
    11401 ttcctcgagg gccggatccg aattcctgta tttgtctgaa aac
//
