LOCUS       sequence-419154-        4412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Young TL, Cepko CL
  TITLE     A role for ligand-gated ion channels in rod photoreceptor 
            development.
  JOURNAL   Neuron. 2004 Mar 25. 41(6):867-79.
  PUBMED    15046720
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2004 Mar 25. 41(6):867-79."
COMMENT     Sequence Label: pGFP Glra2 WA (CC#304)
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(587..603)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(588..604)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(628..647)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(629..647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(654..671)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(654..670)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(663..685)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      812..1267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(899..918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1109..1130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1293..1397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1398..1466,1467..2258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1616..1635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2429..3017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2918..2937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3171..3188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3305..3326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3341..3346,3347..3364,3365..3371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3379..3395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3384..3406
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3403..3419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3403..3419
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3440..3458
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3495..3511
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3495..3511
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 attttgtcct gggtttcctt tgcgataaat atggatgcag cccctgccag ggtcgcactg
       61 ggcatcacca cagtcttaac gatgaccacc cagagttcag gctccagggc atctctgcca
      121 aaggtctcct atgtaaaagc gattgacatc tggatggcgg tgtgccttct gtttgtgttt
      181 gctgccttac tggaatacgc agcggtgaac ttcgtctcca ggcaacacaa ggagttcctg
      241 cgcctccgaa gaagacagaa gaggcagaat aaggaagaag acgttactcg tgaaagtcgt
      301 tttaatttta gcggttatgg gatgggtcac tgcctccaag tgaaagatgg aacagctgtc
      361 aaggccacac ctgccaaccc actcccacaa ccgccaaaag atggagatgc tatcaagaag
      421 aagtttgtgg accgggcaaa aaggattgac acgatatctc gagctgcctt cccattggcc
      481 ttcctcattt tcaacatctt ttactggacc acatacaaga tcattcggca tgaagatgtc
      541 cacaagaaat aggaattcct gcagcccggg ggatcatcaa gcttatcgat accgtcgacc
      601 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacaa ttcactggcc
      661 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      721 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      781 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1021 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1081 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1141 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1201 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     1261 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1321 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1381 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1441 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1501 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1561 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1621 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1681 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1741 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1801 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1861 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1921 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1981 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2041 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2101 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2161 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2221 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3301 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     3361 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     3421 atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
     3481 gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc ctgcaggcct
     3541 cgagggccgg cgcgccgcgg ccgctacatt tcgggatatt ttccacaagc aacacagaaa
     3601 caggaatgaa ccggcagcta gtgaacattt tgacagcctt gtttgcattt ttcttagaga
     3661 caaaccactt caggacggct ttctgcaaag accatgactc caggtctgga aaacaacctt
     3721 cacagaccct atctccttca gatttcttgg acaagttaat gggaaggaca tcaggatatg
     3781 atgcaagaat caggccaaat tttaaaggtc ctccagtaaa cgttacttgc aatattttta
     3841 tcaacagttt tggatcagtc acagaaacga ccatggacta ccgagtgaat atttttctga
     3901 gacaacagtg gaatgattca cggctggcgt acagtgagta cccagatgac tccctggact
     3961 tggacccatc catgctagac tccatttgga aaccagattt gttctttgcc aatgagaagg
     4021 gtgccaactt ccacgatgtc accactgaca acaaattgct acggatttcg aaaaatggca
     4081 aagtgctcta cagtatcaga ctcaccttga ccttatcctg tcccatggac ttgaagaact
     4141 ttccgatgga tgtccagacc tgtacaatgc agctggagag ttttgggtac acgatgaatg
     4201 acctgatatt tgagtggtta agtgatggtc cagtgcaagt tgctgaagga ttgaccctgc
     4261 cccagtttat tttgaaagaa gagaaggaac ttggctactg tacaaagcac tacaacactg
     4321 gaaagtttac ctgcattgag gtcaagtttc acctggaacg ccaaatggga tattatttga
     4381 tccagatgta catcccaagc ctgcttatag ta
//
