LOCUS       sequence-41916-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: HACE1_R (OZ566)
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..67
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(556..575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attgtgcaaa ggccctgaga aaatccaagc tttggcggcc gctgactaca aagacgatga
       61 tgacaaatct agacccgggg agcgcccctt ccagtgtcgc atttgcatgc ggaacttttc
      121 gcagcgttct aacttggcac gtcatacccg tactcatacc ggtgaaaaac cgtttcagtg
      181 tcggatctgt atgcgaaatt tctcccagtc tgcacatttg aaacgtcatc tacgtacgca
      241 caccggcgag aagccattcc aatgccgaat atgcatgcgc aacttcagtt tgaaacatga
      301 cttgcgtcgt cacctaaaaa cccacctgag gggatcctct gcacagtagt aactagagac
      361 tagaaaaagg ccgacaagtc ccgctccgct gaagatcctg gcgtaatagc gaagaggccc
      421 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
      481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      661 acctcgaccc caaaaaactt gattagggtg atggtt
//
