LOCUS       sequence-419165-        9557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging plasmid for v3b PE-eVLP production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: gag-COM-pol
FEATURES             Location/Qualifiers
     source          1..9557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        96..475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     630..650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     676..700
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             837..1253
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1183..1205
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1221..1237
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5844..6218
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     6156..6172
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(6353..6370)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6359..6583
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6657..6676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6658..6676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6683..6700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6683..6699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6692..6714)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6841..7296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6928..6947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7138..7159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7322..7426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7427..7495,7496..8287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7645..7664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8458..9046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8947..8966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9200..9217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9334..9355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9370..9375,9376..9393,9394..9400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9408..9424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9413..9435
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9432..9448
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9432..9448
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9469..9487
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat
       61 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
      121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      661 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      721 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccaagcttgg
      781 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc
      841 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      901 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
      961 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1021 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1081 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1141 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1201 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccaaaccta
     1261 aacctcaagt tctttctgac agtggggggc cgctcatcga cctacttaca gaagaccccc
     1321 cgccttatag ggacccaaga ccaccccctt ccgacaggga cggaaatggt ggagaagcga
     1381 cccctgcggg agaggcaccg gacccctccc caatggcatc tcgcctacgt gggagacggg
     1441 agccccctgt ggccgactcc actacctcgc aggcattccc cctccgcgca ggaggaaacg
     1501 gacagcttca atactggccg ttctcctctt ctgaccttta caactggaaa aataataacc
     1561 cttctttttc tgaagatcca ggtaaactga cagctctgat cgagtctgtc ctcatcaccc
     1621 atcagcccac ctgggacgac tgtcagcagc tgttggggac tctgctgacc ggagaagaaa
     1681 aacaacgggt gctcttagag gctagaaagg cggtgcgggg cgatgatggg cgccccactc
     1741 aactgcccaa tgaagtcgat gccgcttttc ccctcgagcg cccagactgg gattacacca
     1801 cccaggcagg taggaaccac ctagtccact atcgccagtt gctcctagcg ggtctccaaa
     1861 acgcgggcag aagccccacc aatttggcca aggtaaaagg aataacacaa gggcccaatg
     1921 agtctccctc ggccttccta gagagactta aggaagccta tcgcaggtac actccttatg
     1981 accctgagga cccagggcaa gaaactaatg tgtctatgtc tttcatttgg cagtctgccc
     2041 cagacattgg gagaaagtta gagaggttag aagatttaaa aaacaagacg cttggagatt
     2101 tggttagaga ggcagaaaag atctttaata aacgagaaac cccggaagaa agagaggaac
     2161 gtatcaggag agaaacagag gaaaaagaag aacgccgtag gacagaggat gagcagaaag
     2221 agaaagaaag agatcgtagg agacatagag agatgagcaa gctattggcc actgtcgtta
     2281 gtggacagaa acaggataga cagggaggag aacgaaggag gtcccaactc gatcgcgacc
     2341 agtgtgccta ctgcaaagaa aaggggcact gggctaaaga ttgtcccaag aaaccacgag
     2401 gacctcgggg accaagaccc cagacctccc tcctgaccct agatgactcc ggtggcggtg
     2461 gatcaggggg tggttccaaa tcaattcgct gtaaaaactg caacaaactg ttatttaagg
     2521 cggattcctt tgatcacatt gaaatcaggt gtccgcgttg caaacgtcac atcataatgc
     2581 tgaatgcctg cgagcatccc acggagaaac attgtgggaa aagagaaaaa atcacgcatt
     2641 ctgacgaaac cgtgcgttat tccggaggtg gaggtggata gggaggtcag ggtcaggagc
     2701 ccccccctga acccaggata accctcaaag tcggggggca acccgtcacc ttcctggtag
     2761 atactggggc ccaacactcc gtgctgaccc aaaatcctgg acccctaagt gataagtctg
     2821 cctgggtcca aggggctact ggaggaaagc ggtatcgctg gaccacggat cgcaaagtac
     2881 atctagctac cggtaaggtc acccactctt tcctccatgt accagactgt ccctatcctc
     2941 tgttaggaag agatttgctg actaaactaa aagcccaaat ccactttgag ggatcaggag
     3001 ctcaggttat gggaccaatg gggcagcccc tgcaagtgtt gaccctaaat atagaagatg
     3061 agtatcggct acatgagacc tcaaaagagc cagatgtttc tctagggtcc acatggctgt
     3121 ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg actggcagtt cgccaagctc
     3181 ctctgatcat acctctgaaa gcaacctcta cccccgtgtc cataaaacaa taccccatgt
     3241 cacaagaagc cagactgggg atcaagcccc acatacagag actgttggac cagggaatac
     3301 tggtaccctg ccagtccccc tggaacacgc ccctgctacc cgttaagaaa ccagggacta
     3361 atgattatag gcctgtccag gatctgagag aagtcaacaa gcgggtggaa gacatccacc
     3421 ccaccgtgcc caacccttac aacctcttga gcgggctccc accgtcccac cagtggtaca
     3481 ctgtgcttga tttaaaggat gcctttttct gcctgagact ccaccccacc agtcagcctc
     3541 tcttcgcctt tgagtggaga gatccagaga tgggaatctc aggacaattg acctggacca
     3601 gactcccaca gggtttcaaa aacagtccca ccctgtttga tgaggcactg cacagagacc
     3661 tagcagactt ccggatccag cacccagact tgatcctgct acagtacgtg gatgacttac
     3721 tgctggccgc cacttctgag ctagactgcc aacaaggtac tcgggccctg ttacaaaccc
     3781 tagggaacct cgggtatcgg gcctcggcca agaaagccca aatttgccag aaacaggtca
     3841 agtatctggg gtatcttcta aaagagggtc agagatggct gactgaggcc agaaaagaga
     3901 ctgtgatggg gcagcctact ccgaagaccc ctcgacaact aagggagttc ctagggacgg
     3961 caggcttctg tcgcctctgg atccctgggt ttgcagaaat ggcagccccc ttgtaccctc
     4021 tcaccaaaac ggggactctg tttaattggg gcccagacca acaaaaggcc tatcaagaaa
     4081 tcaagcaagc tcttctaact gccccagccc tggggttgcc agatttgact aagccctttg
     4141 aactctttgt cgacgagaag cagggctacg ccaaaggtgt cctaacgcaa aaactgggac
     4201 cttggcgtcg gccggtggcc tacctgtcca aaaagctaga cccagtagca gctgggtggc
     4261 ccccttgcct acggatggta gcagccattg ccgtactgac aaaggatgca ggcaagctaa
     4321 ccatgggaca gccactagtc attctggccc cccatgcagt agaggcacta gtcaaacaac
     4381 cccccgaccg ctggctttcc aacgcccgga tgactcacta tcaggccttg cttttggaca
     4441 cggaccgggt ccagttcgga ccggtggtag ccctgaaccc ggctacgctg ctcccactgc
     4501 ctgaggaagg gctgcaacac aactgccttg atatcctggc cgaagcccac ggaacccgac
     4561 ccgacctaac ggaccagccg ctcccagacg ccgaccacac ctggtacacg gatggaagca
     4621 gtctcttaca agagggacag cgtaaggcgg gagctgcggt gaccaccgag accgaggtaa
     4681 tctgggctaa agccctgcca gccgggacat ccgctcagcg ggctgaactg atagcactca
     4741 cccaggccct aaagatggca gaaggtaaga agctaaatgt ttatactgat agccgttatg
     4801 cttttgctac tgcccatatc catggagaaa tatacagaag gcgtgggttg ctcacatcag
     4861 aaggcaaaga gatcaaaaat aaagacgaga tcttggccct actaaaagcc ctctttctgc
     4921 ccaaaagact tagcataatc cattgtccag gacatcaaaa gggacacagc gccgaggcta
     4981 gaggcaaccg gatggctgac caagcggccc gaaaggcagc catcacagag actccagaca
     5041 cctctaccct cctcatagaa aattcatcac cctacacctc agaacatttt cattacacag
     5101 tgactgatat aaaggaccta accaagttgg gggccattta tgataaaaca aagaagtatt
     5161 gggtctacca aggaaaacct gtgatgcctg accagtttac ttttgaatta ttagactttc
     5221 ttcatcagct gactcacctc agcttctcaa aaatgaaggc tctcctagag agaagccaca
     5281 gtccctacta catgctgaac cgggatcgaa cactcaaaaa tatcactgag acctgcaaag
     5341 cttgtgcaca agtcaacgcc agcaagtctg ccgttaaaca gggaactagg gtccgcgggc
     5401 atcggcccgg cactcattgg gagatcgatt tcaccgagat aaagcccgga ttgtatggct
     5461 ataaatatct tctagttttt atagatacct tttctggctg gatagaagcc ttcccaacca
     5521 agaaagaaac cgccaaggtc gtaaccaaga agctactaga ggagatcttc cccaggttcg
     5581 gcatgcctca ggtattggga actgacaatg ggcctgcctt cgtctccaag gtgagtcaga
     5641 cagtggccga tctgttgggg attgattgga aattacattg tgcatacaga ccccaaagct
     5701 caggccaggt agaaagaatg aatagaacca tcaaggagac tttaactaaa ttaacgcttg
     5761 caactggctc tagagactgg gtgctcctac tccccttagc cctgtaccga gcccgcaaca
     5821 cgccgggccc ccatggcctc accccatatg agatcttata tggggcaccc ccgccccttg
     5881 taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact
     5941 tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag
     6001 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc
     6061 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga
     6121 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga
     6181 aggctgccga ccccgggggt ggaccatcct ctagactgac atggcgcgtt caacgctctc
     6241 aaaacccctt aaaaataagg ttgacgcgtc aacccgcgag gccccctaat ccccttaatt
     6301 cttctgatgc tcagaggggt cagtatcgaa ttcctgcagc cccgctgatc agcctcgact
     6361 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6421 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6481 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6541 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6601 accagctggg gctcgatcct ctagagcggc cgccaccgcg gtggagctcc aattcgccct
     6661 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6721 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6781 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7261 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7441 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7501 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7561 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7621 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7681 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7741 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7801 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7861 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7921 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7981 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8041 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8101 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8161 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8221 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8281 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9301 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9361 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9421 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca
     9481 ctaaagggaa caaaagctgg taccgggccc cccctcgaga tccggccatt agccatatta
     9541 ttcattggtt atatagc
//
