LOCUS       sequence-419189-       12294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CD44 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Du Y, Bradshaw WJ, Leisner TM, Annor-Gyamfi JK, Qian K, Bashore FM, 
            Sikdar A, Nwogbo FO, Ivanov AA, Frye SV, Gileadi O, Brennan PE, 
            Levey AI, Axtman AD, Pearce KH, Fu H, Katis VL
  TITLE     Discovery of FERM domain protein-protein interaction inhibitors for 
            MSN and CD44 as a potential therapeutic approach for Alzheimer's 
            disease.
  JOURNAL   J Biol Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382.
  PUBMED    37866628
REFERENCE   2  (bases 1 to 12294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382."
COMMENT     Sequence Label: pFUW-VenusFlag-hCD44
FEATURES             Location/Qualifiers
     source          1..12294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..3832
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3669..3688
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     3917..3936
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3917..3935
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3952..3954,3955..3957,3958..4668)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3997..4018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4258..4277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4605..4626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4708..4731
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4753..4777
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6875..6899)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6918..6936)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6919..6936)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    6985..7573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7038..7058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7456..7467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7576..7592)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7577..7593)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8098..8278
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8304..8321)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8310..8534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8580..9008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8667..8686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8877..8898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9017..9037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9022..9351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9202..9337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9264..9283
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9399..9446
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9465..9839
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9969..10090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10006..10025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10060..10079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10139..10155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10139..10155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10152..10174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10163..10179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10187..10193,10194..10211,10212..10217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10232..10253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10370..10387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10541..11129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10621..10640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11300..12091,12092..12160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11923..11942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12161..12265)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg
     2641 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     2701 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     2761 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     2821 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     2881 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     2941 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     3001 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     3061 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     3121 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     3181 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     3241 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     3301 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     3361 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     3421 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     3481 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     3541 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     3601 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     3661 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     3721 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     3781 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
     3841 ggctgcaggt cgactctaga ggatccagtt ctctctggct aactagagaa cccactgctt
     3901 actggcttat cgaaattaat acgactcact atagggagac ccaagcttac catggtgagc
     3961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4081 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4141 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4381 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     4441 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     4501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4561 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     4621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg agggggtgga
     4681 tctggagggg gtggatctaa gcttatggac tacaaggacg acgatgacaa gggtggtgga
     4741 tccgaattat caacaagttt gtacaaaaaa gttggcatgg acaagttttg gtggcacgca
     4801 gcctggggac tctgcctcgt gccgctgagc ctggcgcaga tcgatttgaa tataacctgc
     4861 cgctttgcag gtgtattcca cgtggagaaa aatggtcgct acagcatctc tcggacggag
     4921 gccgctgacc tctgcaaggc tttcaatagc accttgccca caatggccca gatggagaaa
     4981 gctctgagca tcggatttga gacctgcagg tatgggttca tagaagggca tgtggtgatt
     5041 ccccggatcc accccaactc catctgtgca gcaaacaaca caggggtgta catcctcaca
     5101 tccaacacct cccagtatga cacatattgc ttcaatgctt cagctccacc tgaagaagat
     5161 tgtacatcag tcacagacct gcccaatgcc tttgatggac caattaccat aactattgtt
     5221 aaccgtgatg gcacccgcta tgtccagaaa ggagaataca gaacgaatcc tgaagacatc
     5281 taccccagca accctactga tgatgacgtg agcagcggct cctccagtga aaggagcagc
     5341 acttcaggag gttacatctt ttacaccttt tctactgtac accccatccc agacgaagac
     5401 agtccctgga tcaccgacag cacagacaga atccctgcta ccagtacgtc ttcaaatacc
     5461 atctcagcag gctgggagcc aaatgaagaa aatgaagatg aaagagacag acacctcagt
     5521 ttttctggat caggcattga tgatgatgaa gattttatct ccagcaccat ttcaaccaca
     5581 ccacgggctt ttgaccacac aaaacagaac caggactgga cccagtggaa cccaagccat
     5641 tcaaatccgg aagtgctact tcagacaacc acaaggatga ctgatgtaga cagaaatggc
     5701 accactgctt atgaaggaaa ctggaaccca gaagcacacc ctcccctcat tcaccatgag
     5761 catcatgagg aagaagagac cccacattct acaagcacaa tccaggcaac tcctagtagt
     5821 acaacggaag aaacagctac ccagaaggaa cagtggtttg gcaacagatg gcatgaggga
     5881 tatcgccaaa cacccagaga agactcccat tcgacaacag ggacagctgc agcctcagct
     5941 cataccagcc atccaatgca aggaaggaca acaccaagcc cagaggacag ttcctggact
     6001 gatttcttca acccaatctc acaccccatg ggacgaggtc atcaagcagg aagaaggatg
     6061 gatatggact ccagtcatag tacaacgctt cagcctactg caaatccaaa cacaggtttg
     6121 gtggaagatt tggacaggac aggacctctt tcaatgacaa cgcagcagag taattctcag
     6181 agcttctcta catcacatga aggcttggaa gaagataaag accatccaac aacttctact
     6241 ctgacatcaa gcaataggaa tgatgtcaca ggtggaagaa gagacccaaa tcattctgaa
     6301 ggctcaacta ctttactgga aggttatacc tctcattacc cacacacgaa ggaaagcagg
     6361 accttcatcc cagtgacctc agctaagact gggtcctttg gagttactgc agttactgtt
     6421 ggagattcca actctaatgt caatcgttcc ttatcaggag accaagacac attccacccc
     6481 agtggggggt cccataccac tcatggatct gaatcagatg gacactcaca tgggagtcaa
     6541 gaaggtggag caaacacaac ctctggtcct ataaggacac cccaaattcc agaatggctg
     6601 atcatcttgg catccctctt ggccttggct ttgattcttg cagtttgcat tgcagtcaac
     6661 agtcgaagaa ggtgtgggca gaagaaaaag ctagtgatca acagtggcaa tggagctgtg
     6721 gaggacagaa agccaagtgg actcaacgga gaggccagca agtctcagga aatggtgcat
     6781 ttggtgaaca aggagtcgtc agaaactcca gaccagttta tgacagctga tgagacaagg
     6841 aacctgcaga atgtggacat gaagattggg gtgtacccaa ctttcttgta caaagtggtt
     6901 gatctagagg gccctattct atagtgtcac ctaaatgcta gagaacctcg agggcgcgcc
     6961 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     7021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7381 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7561 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     7621 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     7681 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7741 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7801 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     7861 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     7921 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     7981 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     8041 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     8101 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8161 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8221 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     8281 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     8341 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8401 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8461 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8521 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     8581 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8641 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8701 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8761 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8821 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8881 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8941 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9001 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9061 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     9121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     9301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     9361 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     9421 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     9481 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     9541 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     9601 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     9661 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     9721 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     9781 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     9841 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     9901 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     9961 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10081 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    10141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    10201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10981 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11041 gctcttgatc cggcaaaaaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    11221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    11281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    12001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    12061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    12121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    12181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacg
//
