LOCUS       sequence-419194-        4684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Renilla luciferase vector for investigating regions controlling 
            transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4684)
  TITLE     Promega Luciferase Plasmids
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL4.82(hRluc/Puro)
FEATURES             Location/Qualifiers
     source          1..4684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..1035
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1076..1197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1087..1106)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1141..1160
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1391..1748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1391..1582
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1414..1434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1599..1734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1661..1680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1779..2378
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2403..2451
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2607..2624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2778..3366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2858..2877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3566..4357,4358..4426))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4531..4579
                     /note="synthetic polyadenylation signal"
     misc_feature    4593..4684
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4633..4652
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
     1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaccgagtac aagcctaccg
     1801 tgcgcctggc cactcgcgat gatgtgcccc gcgccgtccg cactctggcc gccgctttcg
     1861 ccgactaccc cgctacccgg cacaccgtgg accccgaccg gcacatcgag cgtgtgacag
     1921 agttgcagga gctgttcctg acccgcgtcg ggctggacat cggcaaggtg tgggtagccg
     1981 acgacggcgc ggccgtggcc gtgtggacta cccccgagag cgttgaggcc ggcgccgtgt
     2041 tcgccgagat cggcccccga atggccgagc tgagcggcag ccgcctggcc gcccagcagc
     2101 aaatggaggg cctgcttgcc ccccatcgtc ccaaggagcc tgcctggttt ctggccactg
     2161 taggagtgag ccccgaccac cagggcaagg gcttgggcag cgccgtcgtg ttgcccggcg
     2221 tagaggccgc cgaacgcgcc ggtgtgcccg cctttctcga aacaagcgca ccaagaaacc
     2281 ttccattcta cgagcgcctg ggcttcaccg tgaccgccga tgtcgaggtg cccgagggac
     2341 ctaggacctg gtgtatgaca cgaaaacctg gcgcctaatg atctagaacc ggtcatggcc
     2401 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta
     2461 gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     2521 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     2581 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc
     3541 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag
     3601 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga
     3661 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac
     3721 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc
     3781 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa
     3841 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac
     3901 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat
     3961 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa
     4021 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg
     4081 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg
     4141 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc
     4201 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact
     4261 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat
     4321 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg
     4381 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc
     4441 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata
     4501 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg
     4561 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     4621 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     4681 ctct
//
