LOCUS       sequence-419203-       11265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mitochondrial localization of EcSTH-LbNOX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B
  TITLE     Identification of purine biosynthesis as an NADH-sensing pathway to 
            mediate energy stress.
  JOURNAL   Nat Commun. 2022 Nov 17;13(1):7031. doi: 10.1038/s41467-022-34850-0.
  PUBMED    36396642
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-17"; volume: "13(1):7031. doi"; pages: " 
            10.1038/s41467-022-34850-0"
COMMENT     Sequence Label: mito-EcSTH-LbNOX
FEATURES             Location/Qualifiers
     source          1..11265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2555
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2286..2304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2358..2376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2394..2412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2430..2448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2494..2518
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2691..2700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4053..4118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5489..6062
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5512..6064
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5656..5673)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5883..5902
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        6098..6597
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6133..6156)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6530..6549
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6618..7217
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6618..6637)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7114..7134
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7234..7822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7287..7307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7705..7716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8029..8662
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8790..8806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8790..8806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8803..8825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8814..8830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8838..8844,8845..8862,8863..8868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8883..8904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9021..9038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9192..9780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9272..9291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9951..10742,10743..10811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10574..10593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10812..10916)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10964..11098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11001..11020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11055..11074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc catgctcgct acaagggtct
     2581 ttagcctcgt cggaaagaga gctatcagca cctccgtctg cgtgagagct catccccact
     2641 cctacgacta cgacgccatc gtgatcggct ccggccccgg cggcgagggc gccgccatgg
     2701 gcctggtgaa gcagggcgcc cgcgtggccg tgatcgagcg ctaccagaac gtgggcggcg
     2761 gctgcaccca ctggggcacc atcccctcca aggccctgcg ccacgccgtg tcccgcatca
     2821 tcgagttcaa ccagaacccc ctgtactccg accactcccg cctgctgcgc tcctccttcg
     2881 ccgacatcct gaaccacgcc gacaacgtga tcaaccagca gacccgcatg cgccagggct
     2941 tctacgagcg caaccactgc gagatcctgc agggcaacgc ccgcttcgtg gacgagcaca
     3001 ccctggccct ggactgcccc gacggctccg tggagaccct gaccgccgag aagttcgtga
     3061 tcgcctgcgg ctcccgcccc taccacccca ccgacgtgga cttcacccac ccccgcatct
     3121 acgactccga ctccatcctg tccatgcacc acgagccccg ccacgtgctg atctacggcg
     3181 ccggcgtgat cggctgcgag tacgcctcca tcttccgcgg catggacgtg aaggtggacc
     3241 tgatcaacac ccgcgaccgc ctgctggcct tcctggacca ggagatgtcc gactccctgt
     3301 cctaccactt ctggaactcc ggcgtggtga tccgccacaa cgaggagtac gagaagatcg
     3361 agggctgcga cgacggcgtg atcatgcacc tgaagtccgg caagaagctg aaggccgact
     3421 gcctgctgta cgccaacggc cgcaccggca acaccgactc cctggccctg cagaacatcg
     3481 gcctggagac cgactcccgc ggccagctga aggtgaactc catgtaccag accgcccagc
     3541 cccacgtgta cgccgtgggc gacgtgatcg gctacccctc cctggcctcc gccgcctacg
     3601 accagggccg catcgccgcc caggccctgg tgaagggcga ggccaccgcc cacctgatcg
     3661 aggacatccc caccggcatc tacaccatcc ccgagatctc ctccgtgggc aagaccgagc
     3721 agcagctgac cgccatgaag gtgccctacg aggtgggccg cgcccagttc aagcacctgg
     3781 cccgcgccca gatcgtgggc atgaacgtgg gcaccctgaa gatcctgttc caccgcgaga
     3841 ccaaggagat cctgggcatc cactgcttcg gcgagcgcgc cgccgagatc atccacatcg
     3901 gccaggccat catggagcag aagggcggcg gcaacaccat cgagtacttc gtgaacacca
     3961 ccttcaacta ccccaccatg gccgaggcct accgcgtggc cgccctgaac ggcctgaacc
     4021 gcctgttcgg aggaggagga tcaggaggag gagactacaa agaccatgac ggtgattata
     4081 aagatcatga catcgactac aaggatgacg atgacaagat gaaggtcacc gtggtcggat
     4141 gcacccatgc cggcaccttc gccatcaagc aaatcctcgc tgagcaccct gacgccgagg
     4201 tcaccgtcta cgagaggaac gatgtgatct ccttcctgtc ctgtggcatc gccctctacc
     4261 tgggcggaaa agtggccgat ccccaaggcc tcttctacag ctcccctgaa gaactgcaga
     4321 agctgggcgc taatgtgcag atgaaccaca acgtgctggc catcgaccct gaccaaaaga
     4381 ccgtcacagt cgaggacctc accaatcacg cccagaccac cgagtcctac gacaaactgg
     4441 tgatgacctc cggaagctgg cctatcgtgc ccaaaatccc cggcatcgac agcgataggg
     4501 tgaagctctg caagaattgg gcccacgccc aggctctgat tgaggacgcc aaggaggcca
     4561 agaggatcac cgtcatcggc gccggataca tcggagccga actggccgag gcctactcca
     4621 caacaggcca cgacgtcacc ctgattgacg ccatggctag ggtcatgccc aagtacttcg
     4681 atgccgactt caccgacgtc atcgaacagg actacaggga ccatggcgtg caactcgctc
     4741 tgggcgagac agtggagagc ttcaccgaca gcgccaccgg cctcacaatc aagacagaca
     4801 agaactccta tgagaccgac ctggccatcc tctgcattgg ctttaggccc aacacagacc
     4861 tgctgaaagg caaagtggac atggccccta acggcgccat cattaccgac gactacatga
     4921 ggtccagcaa ccctgatatt ttcgctgctg gcgactccgc cgccgtccat tacaacccca
     4981 cacaccaaaa cgcctacatt cccctcgcta ccaacgccgt caggcaggga atcctcgtcg
     5041 gaaagaacct cgtcaagccc acagtgaagt acatgggaac ccagtccagc tccggactgg
     5101 ccctctatga caggacaatt gtctccacag gcctcacact ggccgccgcc aagcaacaag
     5161 gcctcaatgc cgagcaggtc atcgtggagg acaactatag gcccgagttc atgccttcca
     5221 ccgagcccgt cctcatgagc ctggtcttcg accccgatac acacagaatc ctgggaggcg
     5281 ccctgatgtc caaatacgac gtgtcccaga gcgctaatac cctgtccgtc tgcatccaga
     5341 acgagaacac catcgatgac ctggccatgg tggacatgct gttccagccc aatttcgaca
     5401 ggcccttcaa ctacctgaac attctcgccc aggctgccca agctaaagtg gcccaatccg
     5461 tcaacgctta agcggccgcc ccggccggcc cctctccctc ccccccccct aacgttactg
     5521 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5581 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5641 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5701 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     5761 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     5821 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     5881 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     5941 gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa
     6001 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     6061 aatatggccc ccgggacgcg tcatatggaa ttctaccggg taggggaggc gcttttccca
     6121 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     6181 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     6241 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     6301 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     6361 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     6421 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     6481 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     6541 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     6601 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6661 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6721 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6781 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6841 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6901 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6961 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7021 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7081 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7141 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7201 gcaagcccgg tgcctgacgg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt
     7261 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7321 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7381 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7441 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7501 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7561 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7621 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     7681 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7741 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7801 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     7861 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     7921 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     7981 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     8041 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     8101 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     8161 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     8221 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     8281 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     8341 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     8401 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     8461 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8521 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8581 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8641 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8701 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     8761 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8821 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8881 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8941 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9001 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9061 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10921 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
    10981 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11041 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    11101 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    11161 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    11221 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
