LOCUS       sequence-419213-       11674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Argonaute-2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Jouravleva K, Vega-Badillo J, Zamore PD
  TITLE     Principles and pitfalls of high-throughput analysis of 
            microRNA-binding thermodynamics and kinetics by RNA Bind-n-Seq.
  JOURNAL   Cell Rep Methods. 2022 Mar 18;2(3):100185. doi: 
            10.1016/j.crmeth.2022.100185. eCollection 2022 Mar 28.
  PUBMED    35475222
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-03-18"; volume: "2(3):100185. doi"; pages: " 
            10.1016/j.crmeth.2022.100185. eCollection 2022 Mar 28"
COMMENT     Sequence Label: pSCALPS-mAgo2-EGFP
FEATURES             Location/Qualifiers
     source          1..11674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        455..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             671..851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    898..1023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1520..1753
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1937..1981
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2279..2396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2533..2940
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2949..2958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2970..2993
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3003..3023
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    5660..6212
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5804..5821)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6031..6050
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6224..6226,6227..6229,6230..6943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6269..6290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6530..6549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6877..6898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8053..8641
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8106..8126)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8524..8535)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9159..9339
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9410..9998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9490..9509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10169..10960,10961..11029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10792..10811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11030..11134)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11209..11228)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11402..11422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11407..11674,1..62)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(11587..11674,1..48)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11649..11668
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
       61 aaaagctcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      661 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      721 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      781 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      841 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      901 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      961 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1021 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1081 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1141 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1201 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1261 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1321 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     1381 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1441 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1501 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1561 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1621 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1681 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1741 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1801 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1861 tggagtggga cagagaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1921 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1981 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2041 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2101 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2161 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2221 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2281 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2341 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2401 tttattacag ggacagcaga gatccagttt ggttaattaa ctgcagcccc gataaaataa
     2461 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     2521 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
     2581 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
     2641 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
     2701 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
     2761 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
     2821 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
     2881 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
     2941 ggtctagagc caccatggat tacgatatcg attacaagga tgacgacgat aagccaacga
     3001 ccgaaaacct gtattttcag ggcggctctg gtggcagcgg ttactcggga gccggccccg
     3061 ttcttgcttc tcctgctccg acaacatcac ccatcccagg atatgccttc aaacctccac
     3121 ctcggccgga cttcggcacc accgggagaa caatcaaact acaggccaat ttctttgaaa
     3181 tggacatccc caaaattgac atctatcact atgaattgga catcaaacct gagaaatgcc
     3241 ctcggagagt gaacagggaa attgtggagc acatggtcca gcactttaaa actcagatct
     3301 tcggggaccg gaagccagtg tttgatggaa ggaagaatct gtacacagca atgccccttc
     3361 cgatcggcag ggacaaggtg gagctggagg tcacgctccc gggagaaggc aaagatcgca
     3421 tctttaaggt atccatcaag tgggtgtcgt gcgtgagcct gcaggcgtta cacgatgcac
     3481 tttcggggcg gctgcccagc gtccccttcg agacgatcca ggccctggac gttgtcatga
     3541 ggcacttacc atccatgagg tacacccctg ttggccgttc cttcttcact gcatctgaag
     3601 gctgttccaa ccctctgggt gggggcagag aagtgtggtt tggcttccat cagtccgtcc
     3661 gaccttctct ttggaaaatg atgctgaata ttgatgtatc ggcaacagcg ttttacaagg
     3721 cacagccagt gatcgagttt gtttgtgaag ttttggattt taaaagtatt gaagaacaac
     3781 aaaaacctct gacagattcc caaagggtaa agtttaccaa agaaatcaaa ggtctaaagg
     3841 tggagataac gcactgtggt cagatgaaga ggaagtaccg tgtctgcaat gtgacccggc
     3901 ggcctgccag tcaccaaacg ttcccactgc agcaggagag tgggcagaca gtggagtgta
     3961 cagtggccca gtacttcaag gacaggcaca agctggttct gcgctacccc cacctcccgt
     4021 gtttacaagt cggacaggag cagaaacaca cctaccttcc tttggaggtc tgtaacatag
     4081 ttgctggaca gagatgtata aaaaaattaa cagacaatca gacctcaacc atgatcagag
     4141 caactgccag gtcagcacct gatcgccaag aggagatcag caaactgatg cgaagtgcaa
     4201 gtttcaatac agacccatat gttcgtgaat ttggaatcat ggtgaaagat gagatgacag
     4261 atgtgactgg gcgggttctg cagccgccct ccatcctcta cgggggcagg aacaaagcaa
     4321 ttgccacccc tgtccagggt gtgtgggaca tgcgaaacaa gcagttccac acgggcatcg
     4381 agatcaaggt gtgggccatc gcctgcttcg ctccccagcg ccagtgtaca gaagtccatc
     4441 tcaagtcctt cacagagcaa ctgaggaaga tctcgagaga tgctgggatg ccgatccagg
     4501 gtcagccctg cttctgtaaa tacgcacagg gtgcagacag tgtggagccc atgttccgac
     4561 acctgaagaa cacatacgct ggcctccagc tggtggtggt catcctgcct ggcaaaactc
     4621 ctgtgtatgc ggaagtcaag cgtgtgggag acacagtgct ggggatggcc acacagtgcg
     4681 tccagatgaa gaacgtgcag aggaccacgc cacagaccct atccaatctc tgcttaaaga
     4741 tcaatgtcaa actgggaggc gtcaacaaca tcctgctgcc acagggcagg cctccagtgt
     4801 tccagcaacc tgtcatcttc ctgggagccg atgtcaccca cccaccagct ggggatggga
     4861 agaaaccttc tattgctgcc gtcgtgggca gcatggacgc acaccccaac cgctactgtg
     4921 ccaccgtgcg cgtgcagcag caccgacagg agatcatcca ggacctggct gccatggtac
     4981 gagagttgct cattcagttc tacaagtcca cccgcttcaa gcccacccgc atcatcttct
     5041 accgcgatgg cgtctccgag ggccagttcc agcaggttct ccaccatgag ctcctggcca
     5101 tcagagaggc ctgcatcaag ctggagaagg actatcagcc aggaatcacg ttcatcgtgg
     5161 tgcagaagcg gcaccacaca cgcctcttct gcacagacaa aaatgagcgg gttgggaaga
     5221 gtgggaacat tcccgcaggc acaaccgtgg acacgaagat cacccacccc actgagtttg
     5281 acttctacct gtgcagtcac gccggcatcc aggggacaag ccgtccttcc cactaccacg
     5341 tgctttggga tgacaatcgt ttttcttctg atgagctgca gattctgacc taccagctgt
     5401 gtcacaccta cgtgcgctgc acacgctctg tgtcaatacc cgcgccagct tactatgctc
     5461 acctggtggc cttccgagcc aggtaccacc tggtggataa agaacatgac agcgctgaag
     5521 gaagccatac ctctgggcag agcaacgggc gagatcacca agcgctggcc aaggcggtcc
     5581 aggtccacca ggacacactg cgcaccatgt actttgcttg agcggccgcc cagcacagtg
     5641 gctcgagccg cgggttaaca cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     5701 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     5761 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     5821 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5881 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     5941 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     6001 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     6061 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     6121 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     6181 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga
     6241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6301 caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6421 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggcgaactt
     6721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6901 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattct cgaccttgac
     6961 tggcggcgcg accttgaggc ctgcgttcgc ctcagttgcc ccctctgtgc aatggggaga
     7021 cgcgcctcat cgcttgacaa cggccgaaga gccgccgcgc ttccgtctcc cgcgtgcgcg
     7081 cgccatgctg cccacccccg ttccgcactg accctccccc gtgccccgcg tcccgtactg
     7141 ccgccccgcc ccgagtccca tgccgcagcc accgcgacgg agcccgcagg cgggaacctg
     7201 cctccgcgcg ttagcgcgca cgcgcgcctc atgtgtcgtc cccatcagcg ccggcttccg
     7261 tctataggcc agatgcactg tcactctggc gaagtcgcag acccgattgg ccgggacgga
     7321 ggcgcgagac cgggttgcgg gcggggccga acgtggtata aaaggggcgg gaggccaggc
     7381 tcgtgccgtt ttgcagacgc caccgccgag gaaaaccgtg tactattagc cacgcgtgcc
     7441 gccatgaccg aatacaaacc taccgtgagg ctggctacaa gagatgatgt cccaagggct
     7501 gtgagaacac tggccgccgc ttttgccgat taccctgcca cacgccacac tgtggaccca
     7561 gatcggcata tcgagagagt gactgagctg caggaactgt tcctgacccg agtgggcctg
     7621 gacattggga aggtctgggt cgcagacgat ggagcagctg tggctgtctg gaccacacca
     7681 gagagcgtgg aagccggagc tgtctttgca gagatcggcc ctagaatggc agaactgagc
     7741 ggctccaggc tggcagcaca gcagcagatg gagggactgc tggccccaca caggcctaag
     7801 gaaccagcat ggttcctggc taccgtgggg gtctctcctg accatcaggg caaaggactg
     7861 ggaagtgctg tggtcctgcc aggagtggag gctgcagaac gagctggagt ccctgcattt
     7921 ctggagacct ctgctccacg aaacctgccc ttctatgaac ggctgggctt tactgtgacc
     7981 gcagatgtgg aggtccccga aggacctagg acctggtgca tgacacgcaa acccggcgcc
     8041 tgagcgatcg ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8101 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8161 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8221 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8281 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8341 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8401 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     8461 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8521 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8581 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8641 cttaatcgcg tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8701 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8761 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8821 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     8881 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     8941 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     9001 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     9061 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     9121 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     9181 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9241 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9301 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgtttc atgtgagcaa
     9361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11161 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    11221 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
    11281 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
    11341 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgctg
    11401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    11461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    11521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    11581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    11641 atttttttta tttatgcaga ggccgaggcc gcct
//
