LOCUS       sequence-419240-        8738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent vasoactive intestinal 
            peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_VIP1.0
FEATURES             Location/Qualifiers
     source          1..8738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..796
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2029..2050
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2156..2177)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2417..2436)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2937..3510,3511..3513,3514..3522)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3104..3121)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3330..3349
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3523..4230
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3668..3686)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3936..3955
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4288..4317
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4321..4467
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4489..4506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4495..4719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4851..5439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4931..4950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5768..5815
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5840..5859
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6047..6841)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6158..6177)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6768..6787
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6940..6959)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        7038..7229
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7186..7206
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7289..8080,8081..8149))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7912..7931
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8150..8254)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8281..8736
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8368..8387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8578..8599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtcggacgct
      841 gctgcctccc gcgctgctga cctgctggct gctcgccccc gtgaacagca ttcacccaga
      901 atgccgattt catctggaaa tacaggagga agaaacaaaa tgtgcagagc ttctgaggtc
      961 tcaaacagaa aaacacaaag cctgcagtgg cgtctgggac aacatcacgt gctggcggcc
     1021 tgccaatgtg ggagagaccg tcacggtgcc ctgcccaaaa gtcttcagca atttttacag
     1081 caaagcagga aacataagca aaaactgtac gagtgacgga tggtcagaga cgttcccaga
     1141 tttcgtcgat gcctgtggct acagcgaccc ggaggatgag agcaagatca cgttttatat
     1201 tctggtgaag gccatttata ccctgggcta cagtgtctct ctgatgtctc ttgcaacagg
     1261 aagcataatt ctgtgcctct tcaggaagct gcactgcacc aggaattaca tccacctgaa
     1321 cctgttcctg tccttcatcc tgagagccat ctcagtgctg gtcaaggacg acgttctcta
     1381 ctccagctct ggcacgttgc actgccctga ccagccatcc tcctgggtgg gctgcaagct
     1441 gagcctggtc ttcctgcagt actgcatcat ggccaacttc ttctggctgc tggtggaggg
     1501 gctctacctc cacaccctcc tggtggccat gctcccccct agaaggtgct tcctggccta
     1561 cctcctgatc ggatggggcc tccccaccgt ctgcatcggt gcatggactg cggccaggct
     1621 ctacttagaa gacaccggtt gctgggatac aaacgaccac agtgtgccct ggtgggtcat
     1681 acgaataccg attttaattt ccatcatcgt caattttgtc cttttcatta gtattatacg
     1741 aattttgctg cagaagttaa catccccacc cgcgccggcc gggccgcgcg acaccgacgc
     1801 gctggacctg gaggagggag gaaacgtcta tatcaaggcc gacaagcaga agaacggcat
     1861 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
     1921 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1981 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2041 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     2101 cggagggagc atggtgagaa agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2161 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
     2221 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2281 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     2341 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
     2401 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2461 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2521 cctggggcac aagctggagt acaacaccgg agcagcagca cgctggcgcg ggtctcagta
     2581 caagaggctg gccaagtcca cgctcctgct tatcccgctg ttcggcgtcc actacatggt
     2641 gtttgccgtg tttcccatca gcatctcctc caaataccag atactgtttg agctgtgcct
     2701 cgggtcgttc cagggcctgg tggtggccgt cctctactgt ttcctgaaca gtgaggtgca
     2761 gtgcgagctg aagcgaaaat ggcgaagccg gtgcccgacc ccgtccgcga gccgggatta
     2821 cagggtctgc ggttcctcct tctcccgcaa cggctcggag ggcgccctgc agttccaccg
     2881 cggctcccgc gcccagtcct tcctgcaaac ggagacctcg gtcatctagg ctagcgcccc
     2941 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3001 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     3061 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     3121 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     3181 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     3241 ggccaacgcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     3301 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     3361 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     3421 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     3481 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
     3541 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3601 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3661 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     3721 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3781 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3841 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3901 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     3961 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4021 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     4081 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     4141 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     4201 tccaccggcg gcatggacga gctgtacaag gatggtaaaa agaagaaaaa gaagtcaaag
     4261 acaaagtgtg taattatgta agtcgacgaa caaaaactca tctcagaaga ggatctgaat
     4321 gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc ctttaaggtg
     4381 gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct tatcatcctc
     4441 atcatgcttt ggcagaagaa gccacgttag gcggccgctc gagatcagcc tcgactgtgc
     4501 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4561 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4621 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4681 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4741 gtggcggtaa tacggttatc cacagaatcg ggggataacg caggaaagaa catgtgagca
     4801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5161 gagtccaacc cggtaagacg cgacttatcg ccactggcag cagccactgg taacaggatt
     5221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5281 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5521 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5581 agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
     5641 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
     5701 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
     5761 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
     5821 gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc cctagggtgg
     5881 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     5941 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     6001 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     6061 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     6121 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     6181 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     6241 cattttccac catgatattc ggcaagcagg catcgccata ggtcacgacg agatcctcgc
     6301 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     6361 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     6421 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     6481 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     6541 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     6601 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     6661 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     6721 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     6781 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     6841 tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag gcctccaaaa
     6901 aagcctcctc actacttctg gaatagctca gaggccgggc ctcggcctct gcataaataa
     6961 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggaa
     7021 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     7081 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     7141 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     7201 cattccacag ctggttcttt ccgcctcagg actcttcctt tttcaataaa tcaatctaaa
     7261 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7321 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7381 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7441 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7501 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7561 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7621 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7681 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7741 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7801 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7861 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7921 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7981 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8041 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8101 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8161 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8221 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8281 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8341 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8401 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8461 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8521 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8581 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8641 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8701 acgcgaattt taacaaaata ttaacgctta caatttac
//
