LOCUS       sequence-419257-       11635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic 
            point mutation (S1033D), in lentiviral expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Shoyer TC, Gates EM, Cabe JI, Urs AN, Conway DE, Hoffman BD
  TITLE     Coupling during collective cell migration is controlled by a 
            vinculin mechanochemical switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 
            10.1073/pnas.2316456120. Epub 2023 Dec 6.
  PUBMED    38055737
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-12"; volume: "120(50):e2316456120. 
            doi"; pages: " 10.1073/pnas.2316456120. Epub 2023 Dec 6"
COMMENT     Sequence Label: pRRL-VinculinTS-S1033D
FEATURES             Location/Qualifiers
     source          1..11635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             128..172
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    439..555
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        615..994
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        995..1198
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1149..1169
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1243..1261
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3955..4662
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4783..4785,4786..4788,4789..5499)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4828..4849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5089..5108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5436..5457
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6112..6700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6165..6185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6583..6594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6791..7024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7096..7217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7133..7152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7187..7206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7257..7392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7413..7431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7441..7458)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7441..7457)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7450..7472)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7599..8054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7686..7705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7896..7917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8080..8184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8185..8253,8254..9045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8403..8422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9216..9804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9705..9724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9958..9975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10092..10113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10128..10133,10134..10151,10152..10158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10166..10182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10171..10193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10190..10206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10190..10206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10225..10245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10227..10245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10273..10499
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10500..10680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10727..10852
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11345..11578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
       61 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
      121 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
      181 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
      241 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
      301 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
      361 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
      421 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      481 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      541 aaaattcaaa attttatcga tggatccccc gggctcgagt tcgaagatcg atgtacgggc
      601 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      661 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      721 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      781 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      841 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      901 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      961 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     1021 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1081 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1141 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     1201 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
     1261 ggagacccaa gctggctagc gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc
     1321 accatcgaga gcatcttgga gcccgtggct cagcagatct cccacctggt catcatgcac
     1381 gaggaggggg aggtagacgg caaggccatc ccggacctca ccgcccccgt gtcggccgtg
     1441 caggccgctg tcagcaacct ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac
     1501 cagatcttga aaagggatat gccaccagca ttcatcaaag tagagaatgc ctgcaccaag
     1561 ctcgttcgag cagcccagat gctgcaagca gatccttatt cagtaccagc tcgtgactac
     1621 ctaattgatg gatcaagagg catcctttct ggaacatcag acttacttct gacatttgat
     1681 gaagcggagg tccgtaaaat catccgtgtc tgcaaaggaa tattggaata tctgactgtg
     1741 gcagaagtag tagagactat ggaggatttg gtgacatata caaagaatct agggccagga
     1801 atgacaaaga tggcgaaaat gatcgatgag agacaacagg aattaactca tcaggaacat
     1861 agggttatgc tggtgaactc catgaatact gtgaaggagc tattgcctgt acttatttca
     1921 gctatgaaga tctttgtaac cacaaaaaac actaaaagcc agggaataga agaggccttg
     1981 aaaaatcgca atttcacagt agagaaaatg agtgctgaga taaatgaaat aatccgtgta
     2041 ttacaactca cttcctggga tgaagatgcc tgggccagca aggacactga agccatgaaa
     2101 agagctttag ccctaataga ttcaaagatg aaccaggcaa aaggctggct gagagatcca
     2161 aacgcacctc caggggatgc tggtgagcaa gcaataaggc agatccttga tgaagctgga
     2221 aaagcaggag aattgtgtgc aggcaaagaa cgcagggaga ttctgggaac atgcaaaact
     2281 ttgggccaaa tgactgatca acttgctgat ctccgagcta gaggacaggg tgctacaccg
     2341 atggcaatgc agaaggcaca gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg
     2401 gagaatgcag cccggaaatt ggaggccatg acaaactcta agcaggctat tgcaaagaag
     2461 attgatgctg ctcagaactg gcttgcggat cccaacggcg gaagtgaagg agaagaacac
     2521 attcgaggaa ttatgtctga agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa
     2581 agagatgata tccttcgctc cttgggggaa atctctgctc tgacagctaa gctgtcagat
     2641 ctgcgacgac atgggaaagg cgactctcct gaggcccgtg cattggccaa gcaaatagct
     2701 acatcacttc agaacttaca gtccaaaaca aacagagctg tagcaaatac taggccagtt
     2761 aaagctgctg tccatttgga gggcaagatt gagcaagccc agaggtggat agataatcct
     2821 acagttgatg atcggggagt aggccaggca gcaattcggg gtttggttgc agaaggtcgt
     2881 cgtctagcca atgtcatgat gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt
     2941 gtagaccagc tggctgctca gcttgctgac cttgcagcaa gaggggaagg agagtctcct
     3001 caggctaggg caattgctgc tcagcttcag gactccctga aggatctcaa agcacggatg
     3061 caagaagcaa tgacccagga ggtgtctgat gttttcagtg acactacaac ccctattaag
     3121 ttgttagcag tagcagccac tgctccttct gatactccca atagagaaga ggtgtttgaa
     3181 gaaagagcag caaattttga aaaccatgct gctagactgg gagcaacagc agaaaaagca
     3241 gctgcagttg gaactgctaa taaaactact gtggaaggca ttcaggcaac agtcaaatct
     3301 gcaagggagc ttacaccaca ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga
     3361 aatcaagctg cttatgagca ttttgagaca atgaaaaacc aatggattga taatgtagaa
     3421 aagatgacag ggctggtgga tgaggccatc gataccaagt ctctgttgga tgcatcagaa
     3481 gaggctatta agaaggatct tgataaatgt aaagttgcaa tggccaatat gcaacctcag
     3541 atgctggtag ctggagccac cagcattgct agacgagcga accgcatcct gcttgtggca
     3601 aaacgggagg ttgaaaattc agaagaccct aaattcaggg aggctgttaa agcagcttct
     3661 gatgagctga gcaaaaccat atcaccgatg gtaatggatg ctaaagctgt agcaggaaat
     3721 atctctgatc ctggtttgca gaagagtttc ttggattctg gatacaggat tctgggagct
     3781 gtggccaaag tcagagaagc ctttcagcct caggagccag actttccccc tcctcctcct
     3841 gacctagagc atctccatct gactgatgag cttgctcctc caaaaccacc acttccagaa
     3901 ggtgaggttc ccccacccag accaccaccc cctgaagaaa aagatgaggt cgagatggtg
     3961 agcaagggcg aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag
     4021 atggagggca acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc
     4081 tacgacggca ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc
     4141 tacgacattc tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac
     4201 gacatcccca actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg
     4261 accttcgagg acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc
     4321 ttcatctacg agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag
     4381 aagaagacca ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg
     4441 aagggcgacg tcaagcacaa gctgctgctg gagggcggcg gccaccaccg cgttgacttc
     4501 aagaccatct acagggccaa gaaggcggtg aagctgcccg actatcactt tgtggaccac
     4561 cgcatcgaga tcctgaacca cgacaaggac tacaacaagg tgaccgttta cgagagcgcc
     4621 gtggcccgca actccaccga cggcatggac gagctgtaca aggggccagg tggtgcaggg
     4681 ccaggtggtg cagggccagg tggtgcaggg ccaggtggtg cagggcccgg tggtgcaggt
     4741 ccaggtggtg caggtccagg tggtgcaggt ccaggtggtg ctatggtgag caagggcgag
     4801 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4861 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4921 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
     4981 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     5041 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5101 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5161 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5221 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     5281 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     5341 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     5401 aaactgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5461 gccgccggga tcactctcgg catggacgag ctgtacaagg cggccgcgga gttcccagag
     5521 cagaaagcag gagaagctat taatcagccc atgatgatgg ctgctaggca gttgcatgac
     5581 gaggctcgga aatggtctag caagggtaac gacatcattg ctgctgctaa acgaatggcg
     5641 ctgctcatgg ctgagatgtc acgcctggtg cgaggaggca gcggaaacaa gcgtgccctc
     5701 atccagtgtg caaaagatat tgctaaggca tcggatgaag tcactcgatt ggccaaagag
     5761 gtggccaagc aatgtactga taaacgcatt agaacaaacc tcttacaggt ctgtgagcga
     5821 atcccaacca tcagcacgca gctcaaaatt ctttccacag tgaaagctac catgctgggc
     5881 aggactaaca tcagcgatga agaatcagaa caggcaactg agatgttggt tcataacgcc
     5941 cagaacctca tgcaggatgt gaaggaaact gtgagagaag ctgaagcagc atccattaag
     6001 ataagaacag atgccggatt cactctgcgc tgggtcagaa agaccccatg gtatcagtaa
     6061 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagtcga caatcaacct
     6121 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6181 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6241 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6301 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6361 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6421 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6481 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6541 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6601 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6661 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttcgagctcg
     6721 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6781 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7021 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7081 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7201 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     7261 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7321 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7381 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     7441 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7501 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7561 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     7621 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7681 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7741 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7801 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7861 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7921 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7981 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8041 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8101 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8161 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8221 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8281 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8341 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8401 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8461 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8521 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8581 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8641 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8701 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8761 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8821 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8881 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8941 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9001 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9061 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9121 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9361 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9601 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9961 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10021 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10081 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10141 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10201 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
    10261 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
    10321 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
    10381 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
    10441 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
    10501 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10561 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10621 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10681 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    10741 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    10801 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10861 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    10921 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    10981 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    11041 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    11101 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    11161 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
    11221 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    11281 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    11341 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    11401 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    11461 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    11521 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    11581 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgc
//
