LOCUS       sequence-419281-        4236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with mouse EEA1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: pTwist-msEEA1
FEATURES             Location/Qualifiers
     source          1..4236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttcagac gaattctcca aagaactcct ggtcgggtcg ggtctcaagg tagtgacttg
       61 gactcatcag ccacaccaat aaatacagta gacgtaaata atgagagttc atccgagggg
      121 ttcatctgtc ctcaatgtat gaagtccttg ggatcagccg acgagttgtt caagcactac
      181 caagctgtcc acgacgccgg gaatgactcc ggacacggtg gcgaggctgg actcgccctc
      241 acgagggacg acataactct gctccggcaa gaggtccaag accttcaagc ctctttgaaa
      301 gaggagaagt ggtactcaga ggagttgaag aaggagcttg agaagtacca aggattgcaa
      361 caacaagagg ccaagtccga cggattggta acagactcct ctgcagagtt gcaagcactt
      421 gagcaacaac tagaagaggc ccaaactgag aatttcaata taaagcaaat gaaggacttg
      481 ttcgagcaaa aggccgctca acttgctaca gagatcgccg acatcaagtc aaagtacgac
      541 gaggagaaga gtcttcgagc cgctgccgag caaaaggtaa ctcaccttac tgaggacctc
      601 aataagcaaa ctactgtcat ccaagacctc aagacggagt tgctccaacg gcctgggata
      661 gaggacgtag ctgttcttaa gaaggagttg gttcaagttc aaacgttgat ggacaatatg
      721 actttggagc gagagaggga gtcagagaag ttgaaggacg agtgtaagaa gttgcaatcc
      781 gagcacgccc acctcgaggc cactataaat caattgagaa gtgagctcgc caagggacct
      841 caagaggtag cagtctacgt ccaagagatc caaaagctca agggtagtat caatgagctt
      901 acacaaaaga atcaaaatct cactgagaag ctccaaaaga aggacttgga ctacacgcac
      961 ctagaggaga agcacaatga ggagtccgca agtaggaaga ctttgcaagc aagtcttcac
     1021 caaagggacc tcgactgtca acaattgcaa gcccgactca cggcatcaga gtcatctcta
     1081 caacgagcac aaggagagtt gtccgagaag gccgaggccg cacaaaagct ccgagaggag
     1141 ctcagggaag tcgagtcaac tcgacaacac ttgaaggttg aggtaaagca actacaacaa
     1201 caaagagagg agaaggagca acacggattg caattgcaag gcgaggtctc acaactccac
     1261 tgtaagctac tagagacaga gaggcaacta ggggaagccc acgggcgatt gaaggagcaa
     1321 aggcaactca gttctgagaa gctcatggag aaggagcaac aagtcgcaga ccttcaattg
     1381 aagctctcta ggttggaaga gcaattgaag gagaaggtca cgaattccac ggagctacaa
     1441 caccaactcg agaagtctaa gcaacaacac caagagcaac aagcacttca acaatccgct
     1501 actgctaagc ttagggaagc acaaaatgac ctcgagcaag ttctcaggca aataggggac
     1561 aaggaccaaa agatccaaaa tcttgaggct ctccttcaaa agggtaagga aagtgttagt
     1621 ctcctcgaga aggagcgaga ggacttgtac gcaaagatcc aagcaggaga gggagagact
     1681 gccgtcttga atcaattgca agagaagaat cacgcacttc aacaacaact cactcaacta
     1741 acagagaagt tgaagaatca atctgagtcc cacaagcaag ccgaggagaa tcttcacgac
     1801 caagtccaag agcaaaaggc ccacttgcga gcagcacaag acagggtttt gtccttggag
     1861 acgtcagtca gtgagttgtc ttctcaactc aatgagtcca aggagaaggt ctcacaactc
     1921 gacatccaaa tcaaggccaa gactgagctc cttctatccg cagaggccgc taaggccgcc
     1981 caaagggccg acctgcaaaa tcacctagac actgcacaac acgctctcca agacaagcaa
     2041 caagagctca ataaggtctc cgtccaacta gaccaactca cagcaaagtt ccaagagaag
     2101 caagagcact gtatccaact tgagtcccac ctcaaggacc acaaggagaa gcacctctcc
     2161 ctcgagcaaa aggtcgagga ccttgagggt cacataaaga agctcgaggc cgacgcccta
     2221 gaggttaagg cctcaaagga gcaagcgctc caatccctgc aacaacaacg gcaactcagt
     2281 actgacctag agcttagaaa tgcagagcta agtcgggagc ttcaagagca agaagaggtt
     2341 gtctcttgta ctaagcttga ccttcaaaat aagtctgaga tcctcgagaa tataaagcaa
     2401 acattgacaa agaaggaaga ggagaatgtc gttcttaagc aagagttcga gaagctatcc
     2461 caagactcca agactcaaca caaggagttg ggagaccgga tgcaagccgc agtaactgag
     2521 ctcacagctg taaaggcaca aaaggacgca ctccttgccg agctcagtac tacgaaggag
     2581 aagctatcca aggtttcaga ctctctcaag aattctaagt ccgagttcga gaaggagaat
     2641 caaaagggaa aggctgctgt tcttgaccta gagaaggcat gtaaggaact caagcaccaa
     2701 ctccaagtcc aagccgagtc cgcattgaag gagcaagagg acttgaagaa gtccctcgag
     2761 aaggagaagg agacttccca acaattgaag atcgagctca attcagttaa gggtgaggtc
     2821 agtcaagccc aaaatacatt gaagcaaaag gagaaggacg agcaacaact tcaaggaaca
     2881 ataaatcaac tcaagcaaag tgccgagcaa aagaagaagc aaatcgaggc actacaaggc
     2941 gaggttaaga atgcagtttc tcaaaagact gtcctcgaga ataagttgca acaacaatct
     3001 agtcaagcag cacaagagtt ggctgccgag aagggtaagc taagtgccct tcaatctaat
     3061 tacgagaagt gtcaagccga cctcaagcaa ctccaatctg acctttacgg taaggaatca
     3121 gagttgctag caactaggca agacctaaag tccgtcgagg agaagttgac attggcacaa
     3181 gaggacctaa tctctaatag aaatcaaata ggaaatcaaa ataagtctat ccaagagttg
     3241 caagctgcca aggcttctct cgagcaagac tccgcaaaga aagaggccct tctaaaggag
     3301 caatccaagg cactcgagga cgcacaaaga gagaagagtg tcaaggagaa ggagttggtt
     3361 gctgagaagt ccaagttggc agagatggaa gagatcaagt gtcgtcaaga gaaggagatc
     3421 actaagctca atgaggagtt gaagtctcac aagcaagagt ccataaagga gatcacgaat
     3481 ctaaaggacg ccaagcaact actaatccaa caaaagcttg agttgcaagg gcgggtcgac
     3541 tctctcaagg cagctttgga gcaagagaag gagtctcaac aactcatgag ggagcaagta
     3601 aagaaggaag aggagaagag aaaagaggag ttcagtgaga aagaggccaa gcttcactct
     3661 gagatcaagg agaaagaggc tggtatgaag aagcacgagg agaatgaggc aaagttgaca
     3721 atgcaagtaa caactctcaa tgagaatttg gggacggtca agaaggagtg gcaatcctct
     3781 caaagaagag tatccgagct tgagaagcaa actgacgacc ttcggggaga gatcgcagta
     3841 cttgaggcca ctgtccaaaa taatcaagac gagcgtaggg ctctcttgga gcgatgtctc
     3901 aagggtgagg gagagatcga gaagctccaa actaaggccc tagagctaca acgtaagctc
     3961 gacaatacaa cggctgctgt tcaagagttg ggtcgtgaga atcaaagtct ccaaatcaag
     4021 cacacacaag ctcttaatcg aaagtgggcc gaggacaatg aggttcaaaa ttgtatgtct
     4081 tgtggtaagt gtttctccgt cacagttcgg agacaccact gtaggcaatg tggtaatatc
     4141 ttctgtgctg agtgttccac taagaatgca cttactccat cctcaaagaa gcctgtccga
     4201 gtctgtgacg cttgtttcaa tgacctgcaa ggttag
//
