LOCUS       sequence-419288-        1982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Endogenously tag human TMEM115 with a C-terminal 3xHA tag in 
            addition to cytosolic mNG for cell sorting..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
FEATURES             Location/Qualifiers
     source          1..1982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             646..666
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             667..693
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             697..723
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             727..753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             763..819
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             820..1527
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      1072..1081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caggagaggg ggagaattaa taacccttcc cttggatgtg atgccagcac ccaggcagaa
       61 actcctggaa actccagaac ttgccgttcc acccatctgc cctagggcac tagttccact
      121 gacccttaga taccaccctc agaaccattc ctggtggctg gcctcgtggg cagtctggca
      181 gacacccagc tgaccttccc atcccggagg tgcctcagct atggagggaa gattcttaga
      241 gcctttgggt tatcattctg atggcagagt gggtcctaaa ccaatctctg gggagtccca
      301 ggctctgcat ggtgaagggg aaacatagga ttctggggga ggagtctagg tggtacggtg
      361 aggagtcagc ctgctgcgga agggtagagg gcatgagcca aggccaaacc cagagtgacc
      421 tttcctctct cctctggccc caggcaactg gcactcaaag ccctcaatga gcggctgaag
      481 agagtggaag accagtccat ctggcccagc atggatgatg atgaagagga gtctggggcc
      541 aaggtggaca gccccctgcc ctcagacaaa gctcccacac ccccagggaa gggggctgcc
      601 ccagaatcca gtctaatcac cttcgaggca gctcccccga cgctggaaaa cctgtacttc
      661 cagggctacc cgtatgatgt tccggattac gctggctatc cctacgacgt gcccgactat
      721 gccgggtacc cctatgacgt cccagactac gcaggatccg gcgcaacaaa cttctctctg
      781 ctgaaacaag ccggagatgt cgaagagaat cctggaccgg tgagcaaggg cgaggaggat
      841 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg
      901 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac
      961 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg
     1021 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg
     1081 gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt
     1141 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag
     1201 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg
     1261 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt
     1321 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc
     1381 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag
     1441 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg
     1501 atgggcatgg acgagctgta caagtaactc cagaccacct tgagtgtggc acctcccctc
     1561 ccaagccccc cttgacatcc tctcagctac tccagggcac ctgactgctc tgaggagagg
     1621 gaagaaggcc tgctggggct ttccatggcc ttctgctgtt tctcgccaac actacccagg
     1681 actcttgcta cctggttcca actccagaca accactatgc caggcccgga gcctctgagg
     1741 catcggccag tccaggccct catctgaggt aagaatgtac atcagctggc agccccaagc
     1801 aagtggctgc agggacactg atgccacagc tcctgggccg gccctcacat ctgaaactgg
     1861 ttgccgagag ccctgagcca aggcaaggat ttgccaaaaa tgttctgggg gcccagcaaa
     1921 tgcaggagcc gacctggggc tgcacatccc tgcccatccc cagaaagact gttcctgtca
     1981 gg
//
