LOCUS       sequence-419332-        2270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: autoinducible Promoter (PgadB) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPP8
FEATURES             Location/Qualifiers
     source          1..2270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      214..271
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(295..312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(466..1054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(546..565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1237..1331)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1352..2011)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1926..1945
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2173..2191
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2195..2238)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2241..2262
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga gtagttcagc gataaagcct aattggggca aagatcctca ctaaaaactg
       61 gtcgtccatt cgaccagttt ttttgttacc taccatccag attgcgtttt aatttaccct
      121 gttatcacct cgtgctatac tcggcacaac ttataaagat aagggtgacc aaaatgtgag
      181 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
      241 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      661 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      901 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1081 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1201 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1261 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1321 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt
     1381 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1441 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1501 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1561 cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1621 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1681 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1741 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1801 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1861 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1921 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1981 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     2041 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     2101 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     2161 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2221 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt
//
