LOCUS       sequence-419333-        2566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: autoinducible Promoter (PfabZ) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPP7
FEATURES             Location/Qualifiers
     source          1..2566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      510..567
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(591..608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(762..1350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(842..861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1533..1627)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1648..2307)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2222..2241
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2469..2487
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2491..2534)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2537..2558
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga ggcgttgccg tttaggttaa aagttatggg cgttaaaatc ttatggatgt
       61 gatggcttta gactgatgag taatccttta aattaataga tcgtcacaac aattgtgttt
      121 aagaatgcta gttcttagat gcaatttttt ttgcgtgcat ctaagctagt gacagttgaa
      181 cagattgtat cattgcttgc tgaagtcaaa acttgttagc aatttgatta taaactttat
      241 agtcaaattg ctaaactgtg ccagttcgtt gacggattat gaccgcaaaa aatggctcgt
      301 caagaaattt tcgcaaaaaa ataaagctcg attagagccc agcttaccgg gatttggcta
      361 ctaaaaaatg taacatactg taaattaatc tgctgaaatc aattgacaag atttggccgg
      421 acgattaact taatgccatt cccaaacgcc gtgaaaggag cgactaacaa tgtgagacca
      481 ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
      541 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
      601 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      661 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      721 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      781 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      841 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      901 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      961 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1021 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1081 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1141 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1201 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1261 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1321 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1381 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1441 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1501 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     1561 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1621 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcgt cgcagtactg
     1681 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1741 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1801 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1861 gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1921 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1981 gtattcactc cagagtgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2041 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     2101 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2161 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2221 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2281 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2341 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2401 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2461 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     2521 ctaaggatga tttctggaat tcgcggccgc ttctagagcg atgagt
//
