LOCUS       sequence-419334-        3161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector to store Level 0* resistance cassette parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLP5
FEATURES             Location/Qualifiers
     source          1..3161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             169..180
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      894..965
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      981..1008
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1023..1080
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1104..1121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1275..1863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1355..1374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2046..2140)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2180..2971,2972..3040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2803..2822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3041..3145)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcggtctc gtgagacgcg gaaagtgaaa cgtgatttca tgcgtcattt tgaacatttt
       61 gtaaatctta tttaataatg tgtgcggcaa ttcacattta atttatgaat gttttcttaa
      121 catcgcggca actcaagaaa cggcaggttc ggatcttagc tactagagaa agaggagaaa
      181 tactagatgg tttctaaagg tgaagcagtg atcaaagaat ttatgcgctt caaagttcac
      241 atggaaggtt ctatgaacgg ccacgaattt gaaattgaag gtgaaggcga aggtcgtcca
      301 tacgaaggta ctcaaaccgc aaaactgaaa gttaccaaag gtggtccact gccattttct
      361 tgggatattc tgtctccaca atttatgtac ggttctcgtg catttatcaa acacccagca
      421 gatattccag actactacaa acaatctttt ccggaaggtt tcaaatggga acgtgttatg
      481 aattttgaag atggtggtgc agttacggtt acccaagata cctctctgga agatggtact
      541 ctgatctaca aagttaaact gcgtggtact aactttccac cagatggtcc agttatgcag
      601 aaaaaaacca tgggttggga agcatctacc gaacgtctgt acccagaaga tggcgttctg
      661 aaaggtgata tcaaaatggc actgcgtctg aaagatggcg gtcgttacct ggcagatttc
      721 aaaaccacct acaaagcgaa aaaaccagtt caaatgccag gtgcatacaa cgttgatcgt
      781 aaactggata ttaccagcca caacgaagat tacaccgttg ttgaacaata cgaacgttct
      841 gaaggccgtc actctaccgg tggtatggat gaactgtaca aataaccagg catcaaataa
      901 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      961 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacgatcc
     1021 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     1081 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1261 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     2041 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     2101 tggagttctg aggtcattac tggatctatc aacaggagtc caaacgtcgt ctcgcgagac
     2161 cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc tcaccggctc
     2341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2461 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     2521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2761 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     2821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa a
//
