LOCUS       sequence-419335-        2800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: resistance cassette (cat) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPR2
FEATURES             Location/Qualifiers
     source          1..2800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(96..684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(176..195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             1929..1940
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2654..2725
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2741..2768
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
       61 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      841 acttggtctg acagctcgag gcttggggtc tcgtgctgca gtttaaattc ggtcctcggg
      901 atatgataag attaatagtt ttagctatta atcttttttt atttttattt aagaatggct
      961 taataaagcg gttactttgg atttttgtga gcttggacta gaaaaaaact tcacaaaatg
     1021 ctatactagg tagataaaaa tttaggaggc atatcaaatg aactttaata aaattgattt
     1081 agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa cgacttttag
     1141 tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag aaggatataa
     1201 attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata cagcttttag
     1261 aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc cactttatac
     1321 aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa agaatgactt
     1381 caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt cggggaaatt
     1441 gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc catggacttc
     1501 atttactggg tttaacttaa atatcaataa taatagtaat taccttctac ccattattac
     1561 agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt tacaggtaca
     1621 tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc aggaattgtc
     1681 agataggcct aatgactggc ttttataata tgagataatg ccgactgtac ttggatccta
     1741 aacgcaattg atgattggtt cggaaggggt ctgagacccg gaaagtgaaa cgtgatttca
     1801 tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa ttcacattta
     1861 atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc ggatcttagc
     1921 tactagagaa agaggagaaa tactagatgg tttctaaagg tgaagcagtg atcaaagaat
     1981 ttatgcgctt caaagttcac atggaaggtt ctatgaacgg ccacgaattt gaaattgaag
     2041 gtgaaggcga aggtcgtcca tacgaaggta ctcaaaccgc aaaactgaaa gttaccaaag
     2101 gtggtccact gccattttct tgggatattc tgtctccaca atttatgtac ggttctcgtg
     2161 catttatcaa acacccagca gatattccag actactacaa acaatctttt ccggaaggtt
     2221 tcaaatggga acgtgttatg aattttgaag atggtggtgc agttacggtt acccaagata
     2281 cctctctgga agatggtact ctgatctaca aagttaaact gcgtggtact aactttccac
     2341 cagatggtcc agttatgcag aaaaaaacca tgggttggga agcatctacc gaacgtctgt
     2401 acccagaaga tggcgttctg aaaggtgata tcaaaatggc actgcgtctg aaagatggcg
     2461 gtcgttacct ggcagatttc aaaaccacct acaaagcgaa aaaaccagtt caaatgccag
     2521 gtgcatacaa cgttgatcgt aaactggata ttaccagcca caacgaagat tacaccgttg
     2581 ttgaacaata cgaacgttct gaaggccgtc actctaccgg tggtatggat gaactgtaca
     2641 aataaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2701 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2761 tttctgcgtt tatacagctc actcaaaggc ggtaatacgg
//
