LOCUS       sequence-419339-        2333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: Terminator (TpepN) for the construction of 
            Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPT2
FEATURES             Location/Qualifiers
     source          1..2333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      277..334
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(358..375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(529..1117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(609..628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1300..1394)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1415..2074)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1989..2008
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2236..2254
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2258..2301)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2304..2325
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct tcaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg
       61 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
      121 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaag
      181 cgagaataag gaagataaat cccataaggg cgggagcaga atgtccgaga ctaatcgctt
      241 gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
      301 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      361 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      721 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      961 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1201 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1261 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1321 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1381 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcgtcgc
     1441 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1501 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1561 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1621 tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1681 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1741 cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1801 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1861 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1921 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1981 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2041 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2101 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2161 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     2221 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2281 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg agt
//
