LOCUS       sequence-419345-        2165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0* Part: 5' Connector (5C_07_5C1C) for the 
            construction of Level 2 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPC54
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      41..98
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(122..139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(293..881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(373..392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1064..1158)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1184..1975,1976..2044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1807..1826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2045..2149)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtctcgaac aggtctcggg agggagtgag acggatccag tccggcaaaa aagggcaagg
       61 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
      121 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      181 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      241 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
      301 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      361 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      481 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      661 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      721 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      781 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      841 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      901 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      961 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1021 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
     1081 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1141 gatctatcaa caggagtcca aacgtgtaaa cttggtctga cagttaccaa tgcttaatca
     1201 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1261 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1321 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1381 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1441 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     1501 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1561 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1621 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1681 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1741 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1801 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1861 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1921 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1981 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2041 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2101 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2161 gaaaa
//
