LOCUS       sequence-419346-        2842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0* Part: replication origin (256rep) for the 
            construction of Level 2 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPO5
FEATURES             Location/Qualifiers
     source          1..2842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      718..775
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(799..816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(970..1558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1050..1069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1741..1835)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1861..2652,2653..2721))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2484..2503
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2722..2826)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtctcgtgt gaaggattat tcggctggtt gcgacgttaa aatgataaag gttgtattaa
       61 tcttatatta cggttataat gtactcaact taataaatga acgcaaaaaa aagaaccctc
      121 aacttagcag agttaggatt cacgacttat cagcacaacc tgataagatt ttcgatagca
      181 agtactacca atacaagcta tctaacttgg tactattata acatgtaggc taagtttttc
      241 aaccattgat acttaaagta aacggttgtt atcgggaatc ttaacagaaa cctgatagca
      301 accgtttttt tgttattcaa tggttagcaa ccatcaaagc aactaaaggc tggaaacctg
      361 ttcttagcta gtaaaacctc ccgtgagtgt cgttcgtgac cccgcttgca gttaacaaca
      421 taggtatgct aaaccttgtc gagatcaacg cgactaaaga cgtggctgga agactaggaa
      481 atgatacgga caggctaact attaacgcag attattcggg ttgctgctaa aaccaactct
      541 aataatagtt agtgcaaggg ctggttgagc ttaaattgtc tgataaagag ttctctcttt
      601 atactgcaaa agaagcgcag ttattcacga ttaggataac tgtttgagag agcctaaggg
      661 cttgaccctt gatggtttaa gcaccgctat gcgtgcgggt gcttgagacg gatccagtcc
      721 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      781 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      961 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1381 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1741 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1801 ttctgaggtc attactggat ctatcaacag gagtccaaac gtgtaaactt ggtctgacag
     1861 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1921 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1981 cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa
     2041 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2101 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2161 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2221 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2281 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2341 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2401 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2461 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     2521 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2581 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2641 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2701 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2761 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2821 tccgcgcaca tttccccgaa aa
//
