LOCUS       sequence-419370-        6219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for
            human PRRX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Kim S, Morgunova E, Naqvi S, Goovaerts S, Bader M, Koska M, Popov A,
            Luong C, Pogson A, Swigut T, Claes P, Taipale J, Wysocka J
  TITLE     DNA-guided transcription factor cooperativity shapes face and limb 
            mesenchyme.
  JOURNAL   Cell. 2024 Feb 1;187(3):692-711.e26. doi: 
            10.1016/j.cell.2023.12.032. Epub 2024 Jan 22.
  PUBMED    38262408
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.12.032"; journalName: "Cell"; date: "2024-02-1-
            1"; volume: "187"; issue: "3"; pages: "692-711.e26"
COMMENT     Sequence Label: pAAV_FKBP-V5-PRRX1
FEATURES             Location/Qualifiers
     source          1..6219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   826..955
                     /label=AAV2 ITR
     primer_bind     complement(1011..1030)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(2006..2008,2009..2329)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             2345..2386
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     repeat_region   3324..3453
                     /label=AAV2 ITR
     rep_origin      3824..4337
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3911..3930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4121..4142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4819..4838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4938..4960
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4998..5016)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5084..5188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5189..5257,5258..6049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5407..5426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg cgctcgctcg
      841 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
      901 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
      961 ttaatgatta acccgccatg ctacttatct acgtagccat gctctagcgg cctcggcctc
     1021 tgcataaata aaaaaaatta gtcagccatg agcttggccc attgcatacg ttgtatccat
     1081 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     1141 tattgactag gtgccagaga gcttcttgat ccaactgaga aggaaaaagg agcccagcaa
     1201 gaagaggggg agagagagaa ggggaaaggg gggaacccac cagcaccctc cgtcggactc
     1261 ttgaagcctt ttttttttaa ttcttaattt ttttttttac tctttacaaa aagtaaagtg
     1321 agaatcctgc tctctaatac atctgcaaga catcaccctc tcctcctgaa actttagtca
     1381 ctcctgagaa tccacaggag tgcagagagg ggggaacacg ttttcttgaa gatgttttaa
     1441 agctggaaca agccttcttc tgttggtgct tgaactcttg cctgggaata acttttttaa
     1501 cctttaaaaa aaccattcac tttgattctt ctctcccacc ccttcttctc tcttcttctg
     1561 tttgcctaac tcccccgccc tgctggcctc cgctttcctc tctccccctt gttattattt
     1621 ttagtctgtg cgtgtggaca cttttggaga gttggaaggg atttttttct cctgacttga
     1681 acatagggtg actttttaat attgtatttt actgtggatt atctctttgg accgcgccgg
     1741 acttggcctc aggaaatcaa ccaatgctgc ggaaggcggc tggtgcacaa cgctctgctc
     1801 tacagaaggg ggtcccccac cctcttttcc aatttttttt ttttggcctt cctctccttc
     1861 cctccctctt cctccctctc tctctctctc tctccactac ccccctcttt cttccccact
     1921 cggctcctct cccccctcgc gcccacagcg tttggtgttg attcgagcgg gaagaggggg
     1981 gtgggtggga tcggtggggg agaccatggg agtgcaggtg gaaaccatct ccccaggaga
     2041 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga
     2101 agatggaaag aaagttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg
     2161 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag
     2221 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat
     2281 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaat ctggatccgg
     2341 ttccggcaag cccatcccca accccctgct gggcctggac agcacctccg gttcaggctc
     2401 tggatccggt tccacctcca gctacgggca cgttctggag cggcaaccgg cgctgggcgg
     2461 ccgcttggac agcccgggca acctcgacac cctgcaggcg aaaaagaact tctccgtcag
     2521 tcacctgcta gacctggagg aagccgggga catggtggcg gcacaggcgg atgagaacgt
     2581 gggcgaggct ggccggagcc tgctggagtc gccgggactc accagcggca gcgacacccc
     2641 gcagcaggac agtgagtgag gggcgcatgc ccacgggggt gtgtgcccgg gacagagggc
     2701 ggggacccgt gtagggcagc tagagcccgt ccgcggccag aaagacaagg tcctgggacc
     2761 aggagaggtg atggcagact tcaaaggaag ggccagtcac aggggaaacc agatgagaaa
     2821 agcgggtctc ggccgagggc atagccaagg aggacgctgg agagaggctc caaggtccgg
     2881 ggacactccg aggccctgcg gtctcaggca gggactctgt ccgcggcgga ctctctttac
     2941 ttcggctagg caccggctcg gttcatcccg cacgcggggc ggcggggctg ggctgggaaa
     3001 aggtcttagc ggggtgcaaa ctcagagagc cagcgctgtt tgggaaagca gcaggttaaa
     3061 gatgctgagt cgggttggaa gggcttctct cgggcagcgt atgacggctt gagggagggc
     3121 accaggcaca gggagcagga cttggcgctg ctcaggctgc actctccacg cgggcaccgt
     3181 aaggccgggc gcgaccggga ggccccgaga gaacgcccgg ccccagacgc tggagctgct
     3241 ctctgccccc tgctgttgcc tctaccgcaa actagagcat ggctacgtag ataagtagca
     3301 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     3361 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3421 ccgggcggcc tcagtgagcg agcgagcgcg cagctggcgt aatagcgaag aggcccgcac
     3481 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgattcc gttgcaatgg
     3541 ctggcggtaa tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc
     3601 aggcaagtga tgttattact aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg
     3661 gacagactct tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg
     3721 taccgttcct gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta
     3781 acgaggaaag cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc
     3841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4261 atattaacgc ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc
     4321 tgattatcaa ccggggtaca tatgattgac atgctagttt tacgattacc gttcatcgat
     4381 tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa
     4441 aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg
     4501 gtgatttgac tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag
     4561 gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg
     4621 cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat
     4681 gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg
     4741 atgttggaat cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4801 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4861 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4921 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4981 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5041 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5161 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5221 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5281 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5341 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5401 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5461 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5521 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5581 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5641 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5701 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5761 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5821 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5881 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5941 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6001 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6061 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
