LOCUS       sequence-419371-        2171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: ribosome binding site (L. plantarum 
            optimized) for the construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPS2
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      115..172
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(196..213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(367..955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(447..466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1138..1232)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1253..1912)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1827..1846
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2074..2092
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2096..2139)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2142..2163
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac tttagaaatg cattgatgcg aaattgatgt aattcttgaa aacatgtaca
       61 aggaggtttt acaaatgtga gaccactcat cgcgactagt agcggccgct gcagtccggc
      121 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      181 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      361 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      781 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     1141 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1201 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1261 cgccctgcca ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1321 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1381 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1441 aatcaaaact ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa
     1501 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1561 gtagaaactg ccggaaatcg tcgtggtatt cactccagag tgatgaaaac gtttcagttt
     1621 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1681 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1741 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1801 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1861 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1921 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1981 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
     2041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     2101 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta
     2161 gagcgatgag t
//
