LOCUS       sequence-419376-        2153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: constitutive Promoter (P40) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPP4
FEATURES             Location/Qualifiers
     source          1..2153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      97..154
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(178..195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(349..937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(429..448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1120..1214)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1235..1894)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1809..1828
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2056..2074
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2078..2121)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2124..2145
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga gtaccggaga ttgttcaaca tacatggatc gtggtatgtt tccaatactt
       61 gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
      121 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      781 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1081 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1141 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1201 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcgtcgc
     1261 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1321 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1381 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1441 tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1501 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1561 cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1621 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1681 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1741 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1801 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1861 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1921 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1981 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg agt
//
