LOCUS       sequence-419377-        2797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: replication origin (256rep) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPO1
FEATURES             Location/Qualifiers
     source          1..2797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      741..798
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(822..839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(993..1581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1073..1092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1764..1858)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1879..2538)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2453..2472
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2700..2718
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2722..2765)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2768..2789
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgagc taaggattat tcggctggtt gcgacgttaa aatgataaag gttgtattaa
       61 tcttatatta cggttataat gtactcaact taataaatga acgcaaaaaa aagaaccctc
      121 aacttagcag agttaggatt cacgacttat cagcacaacc tgataagatt ttcgatagca
      181 agtactacca atacaagcta tctaacttgg tactattata acatgtaggc taagtttttc
      241 aaccattgat acttaaagta aacggttgtt atcgggaatc ttaacagaaa cctgatagca
      301 accgtttttt tgttattcaa tggttagcaa ccatcaaagc aactaaaggc tggaaacctg
      361 ttcttagcta gtaaaacctc ccgtgagtgt cgttcgtgac cccgcttgca gttaacaaca
      421 taggtatgct aaaccttgtc gagatcaacg cgactaaaga cgtggctgga agactaggaa
      481 atgatacgga caggctaact attaacgcag attattcggg ttgctgctaa aaccaactct
      541 aataatagtt agtgcaaggg ctggttgagc ttaaattgtc tgataaagag ttctctcttt
      601 atactgcaaa agaagcgcag ttattcacga ttaggataac tgtttgagag agcctaaggg
      661 cttgaccctt gatggtttaa gcaccgctat gcgtgcgggt gcttgagacc actcatcgcg
      721 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
      781 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      841 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      901 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      961 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     1021 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1081 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1141 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1201 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1261 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1321 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1381 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1441 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1501 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1621 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1681 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1741 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     1801 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1861 agcgagctcg atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc
     1921 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     1981 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2041 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2101 tgatacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2161 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2221 ccagagtgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2281 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     2341 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2401 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2461 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2521 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2581 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     2641 caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2701 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
     2761 atttctggaa ttcgcggccg cttctagagc gatgagt
//
