LOCUS       sequence-419379-        5209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: replication origin (pAMBeta1) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPO3
FEATURES             Location/Qualifiers
     source          1..5209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3153..3210
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3234..3251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3405..3993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3485..3504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4176..4270)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4291..4950)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4865..4884
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     5112..5130
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(5134..5177)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    5180..5201
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgagc tgctcgagat ctatcgataa gcttaagtga ttagtcaaag aatggtgatg
       61 acaattgtaa attctattta atcactttga ctagcaaata ctaacaacaa gacacacaca
      121 ccaaaaatca aaaattcact acttttagtt aaaaaccacg taaccacaag aactaatcca
      181 atccatgtaa tcgggttctt caaatatttc tccaagattt tcctcctcta atatgctcaa
      241 cttaaatgac ctattcaata aatctattat gctgctaaat agtttatagg acaaataagt
      301 atactctaat gacctataaa agatagaaaa ttaaaaaatc aagtgttcgc ttcgctctca
      361 ctgcccctcg acgttttagt agcctttccc tcacttcgtt cagtccaagc caactaaaag
      421 ttttcgggct actctctcct tctcccccta ataattaatt aaaatcttac tctgtatatt
      481 tctgctaatc attcgctaaa cagcaaagaa aaaacaaaca cgtatcatag atataaatgt
      541 aatggcatag tgcgggtttt attttcagcc tgtatcatag ctaaacaaat cgagttgtgt
      601 gtccgtttta gggcgttctg ctagcttgtt taaagtctct tgaatgaatg tatgctctaa
      661 gtcaaaagaa tttgtcagcg cctttatata gctttctttt tcttcttttt ttactttaat
      721 gatcgatagc aacaatgatt taacactagc aagttgaatg ccaccatttc ttcctggttt
      781 aatcttaaag aaaatttcct gattcgcctt cagtaccttc agcaatttat ctaatgtccg
      841 ttcaggaatg cctagcactt ctctaatctc ttttttggtc gtcactaaat aaggcttgta
      901 tacatcgctt ttttcgctaa tataagccat taaatcttct ttccattctg acaaatgaac
      961 acgttgacgt tcgcttcttt ttttcttgaa tttaaaccac ccttgacgga caaataaatc
     1021 tttactggtt aaatcacttg atacccaagc tttgcaaaga atggtaatgt attccctatt
     1081 agccccttga tagttttctg aataggcact tctaacaatt ttgattactt ctttttcttc
     1141 taagggttga tctaatcgat tattaaactc aaacatatta tattcgcacg tttcgattga
     1201 atagcctgaa ctaaagtagg ctaaagagag ggtaaacatg acgttattac gccctattaa
     1261 acccttttct cctgaaaatt tcgtttcgtg caataagaga ttaaaccagg gttcatctac
     1321 ttgttttttg ccttctgtac cgcttaaaac cgttagactt gaacgagtaa agcccttatt
     1381 atctgtttgt ttgaaagacc aatcttgcca ttctttgaaa gaataacggt aattaggatc
     1441 aaaaaattct acattgtccg ttcttggtat gcgagcaata ccaaaatgat tacacgttag
     1501 atcaactggc aaagactttc caaaatattc tcggatattt tgcgaaatta ttttggctgc
     1561 tttgacagat ttaaattctg attttgaagt cacatagact ggcgtttcta aaacaaaata
     1621 tgcttgataa cctttatcag atttgataat catagtaggc ataaaaccta aatcaatagc
     1681 ggttgttaaa atatcgcttg ctgaaatagt ttcttttgcc gtgtgaatat caaaatcaat
     1741 aaagaaggta ttgatttgtc ttaaattgtt ttcagaatgt cctttcgtgt atgaacggtt
     1801 ttcgtctgca tacgttccat aacgataaac gttgggtgtc caatgtgtaa atgtatcttg
     1861 attttcttga atcgcttcct cggaagtcag aacaacacca cgaccgccaa tcatgcttga
     1921 ttttgagcga tacgcaaaaa tagccccttt gcttttacct ggcttggtag tgattgagcg
     1981 aattttacta tttttaaatt tgtactttaa caagccgtca tgaagcacag tttctacaac
     2041 aaaagggata ttcattcagc tgttctcctt tcctataaat cctataaaat aggttgttta
     2101 attaacttgg tttgcttttt cattcaactg tttcaatatt gcatgttttg aaaaagattt
     2161 ttttccttta taagtcaatt tttttccact aatcgaataa attattttgt tattttctat
     2221 taacttatat atataatctt ccccctccga agaaaaatac ttatctgatt ttgtttctaa
     2281 gtagatattt ctcttttcta actctttctt aaacgtttct agtgtataga tatttgctaa
     2341 ttttcttatc tccaataaac tattttttat ataagtttta cattcatcat gattcataca
     2401 aactccacct tctataaatg aatacaaaaa aagcaatcaa acgatttccg attgattgct
     2461 taacaattct taaattcagt agcttagata cttgaaaact ctctgatttc cctatataat
     2521 gatagtacgg ttatataccg tcttcaaaca aagttaatta aataacttct tacgagggaa
     2581 gagttcatct gactaactga taagcgttgg tttggcaatc ttatcgggct atgcatttat
     2641 aaaatgtcgt caaacatttt ataaatgtgt catggctctt ttttcgtttc tattcagttc
     2701 gttgtttcgt tatatctagt ataccgcttt taaaaaaaat aagcaacgat ttcgtgcatt
     2761 attcacacga agtcattgct tttttcttct tccatttcta aatccaatgt tacttgttct
     2821 gattctgttt ctggttctgg ttctgttggc tcatttggga ttaaatccac tactagcgtt
     2881 gagttagtta actttgcaat ttgttctagt gtttttatgg ttggatctga ttttcctgat
     2941 tctattcgtg aataatttga tctactcatt tctaattctt ggggtaccgc cagcatttcg
     3001 gaaaaaaacc acgctaagga ttttttctat aaaaagagcc gttatattaa gaataaaacg
     3061 gctcttttat acgtaaagga cgtaaattca tttgcccagt gtcatgtaat ctgcttgaga
     3121 ccactcatcg cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
     3181 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     3241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     3421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4141 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
     4201 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     4261 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact cgtcgcagta
     4321 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     4381 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     4441 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     4501 ccagggattg gctgatacga aaaacatatt ctcaataaac cctttaggga aataggccag
     4561 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     4621 gtggtattca ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     4681 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     4741 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     4801 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     4861 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     4921 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     4981 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     5041 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat
     5101 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     5161 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gcgatgagt
//
