LOCUS       sequence-419380-        2124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: mock / negative control Promoter (P-nc) for 
            the construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPP1
FEATURES             Location/Qualifiers
     source          1..2124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..125
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(149..166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(320..908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(400..419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1091..1185)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1206..1865)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1780..1799
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2049..2092)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2095..2116
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga gcccctggcg cccctttact tgagaccact catcgcgact agtagcggcc
       61 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      121 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      301 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
      361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      721 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1081 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1141 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1201 tcaaattacg ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg
     1261 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1321 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1381 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac
     1441 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1501 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa
     1561 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1621 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1681 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1741 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1801 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1861 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1921 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     2101 gcggccgctt ctagagcgat gagt
//
