LOCUS       sequence-419382-        2156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: constitutive Promoter (P25) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPP3
FEATURES             Location/Qualifiers
     source          1..2156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      100..157
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(181..198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(352..940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(432..451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1123..1217)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1238..1897)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1812..1831
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2059..2077
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2081..2124)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2127..2148
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga ggtggggtta gttgttgaca ttaaggcaca tcattgatat ggtttaatta
       61 cttgagacca ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
      121 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
      181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
      361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1081 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     1141 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1201 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcgt
     1261 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1321 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     1381 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     1441 aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat
     1501 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1561 aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc agtttgctca tggaaaacgg
     1621 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1681 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1741 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1801 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1861 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1921 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1981 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     2041 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     2101 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagcg atgagt
//
