LOCUS       sequence-419383-        2402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: 5' Connector (5C1CSN with double terminator 
            Tcat+sai) for the construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPC53
FEATURES             Location/Qualifiers
     source          1..2402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      346..403
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(427..444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(598..1186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(678..697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1369..1463)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1484..2143)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2058..2077
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2305..2323
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2327..2370)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2373..2394
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaac acgtctcggg agtacgccaa atgatcccag taaaaagcca cccgcatggc
       61 gggtggcttt ttattagccc tagaagggct tcccacacgc atttcagcgc cttagtgcct
      121 tagtttgtga atcataggtg gtatagtccc gaaatacccg tctaaggaat tgtcagatag
      181 gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta cagtcggttt
      241 tctaatgtca ctaacctgcc ccgttagttg aagaaggttt ttatattaca gctccagatc
      301 tacgggagtg agaccactca tcgcgactag tagcggccgc tgcagtccgg caaaaaaggg
      361 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca
      421 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      601 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      901 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      961 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1021 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1081 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1141 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1201 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1261 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1321 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat
     1381 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1441 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1501 actcgtcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1561 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1621 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1681 tggtgaaact cacccaggga ttggctgata cgaaaaacat attctcaata aaccctttag
     1741 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1801 gccggaaatc gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga
     1861 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1921 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1981 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2041 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2101 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2161 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2221 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt
     2281 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     2341 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagcgatga
     2401 gt
//
