LOCUS       sequence-419387-        6740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Nicolaides NC, Littman SJ, Modrich P, Kinzler KW, Vogelstein B
  TITLE     A naturally occurring hPMS2 mutation can confer a dominant negative 
            mutator phenotype.
  JOURNAL   Mol Cell Biol. 1998 Mar . 18(3):1635-41.
  PUBMED    9488480
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Mar . 18(3):1635-41."
COMMENT     Sequence Label: pSG5 PMS2-wt
FEATURES             Location/Qualifiers
     source          1..6740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1097..1218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1134..1153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1188..1207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1252..1269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1423..2011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1503..1522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2182..2973,2974..3042))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2805..2824
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3043..3147)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3215..3233
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3271..3293)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3393..3412
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3479..3934)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3616..3637)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3828..3847
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4060..4082
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4074..4091
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4075..4091
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4128..4148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4236..4446
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4258..4454
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4305..4440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4485..5057
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     5065..5084
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5112..5131
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5112..5130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaacatgtgg actctcagga gaaagcgcct gaaactgacg actctttttc agatgtggac
       61 tgccattcaa accaggaaga taccggatgt aaatttcgag ttttgcctca gccaactaat
      121 ctcgcaaccc caaacacaaa gcgttttaaa aaagaagaaa ttctttccag ttctgacatt
      181 tgtcaaaagt tagtaaatac tcaggacatg tcagcctctc aggttgatgt agctgtgaaa
      241 attaataaga aagttgtgcc cctggacttt tctatgagtt ctttagctaa acgaataaag
      301 cagttacatc atgaagcaca gcaaagtgaa ggggaacaga attacaggaa gtttagggca
      361 aagatttgtc ctggagaaaa tcaagcagcc gaagatgaac taagaaaaga gataagtaaa
      421 acgatgtttg cagaaatgga aatcattggt cagtttaacc tgggatttat aataaccaaa
      481 ctgaatgagg atatcttcat agtggaccag catgccacgg acgagaagta taacttcgag
      541 atgctgcagc agcacaccgt gctccagggg cagaggctca tagcacctca gactctcaac
      601 ttaactgctg ttaatgaagc tgttctgata gaaaatctgg aaatatttag aaagaatggc
      661 tttgattttg ttatcgatga aaatgctcca gtcactgaaa gggctaaact gatttccttg
      721 ccaactagta aaaactggac cttcggaccc caggacgtcg atgaactgat cttcatgctg
      781 agcgacagcc ctggggtcat gtgccggcct tcccgagtca agcagatgtt tgcctccaga
      841 gcctgccgga agtcggtgat gattgggact gctcttaaca caagcgagat gaagaaactg
      901 atcacccaca tgggggagat ggaccacccc tggaactgtc cccatggaag gccaaccatg
      961 agacacatcg ccaacctggg tgtcatttct cagaactgac cgtagtcact gtatggaata
     1021 attggtttta tcgcagattt ttatgttttg aaagacagag tcttcactaa cctggatcca
     1081 gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1201 actcatcaat gtatcttatc atgtctggtc gactctagac tcttccgctt cctcgctcac
     1261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2461 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3061 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3181 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3241 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3301 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3361 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3421 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     3481 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     3541 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     3601 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3661 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3721 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3781 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3841 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3901 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     3961 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     4021 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     4081 cgacggccag tgaattgtcg acttctgagg cggaaagaac cagctgtgga atgtgtgtca
     4141 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4201 caattagtca gcaaggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4261 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4321 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4381 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aagaggcttt tttggaggcc
     4441 taggcttttg caaaaagctc cggatcgatc ctgagaactt cagggtgagt ttggggaccc
     4501 ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt
     4561 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat
     4621 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat
     4681 tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt
     4741 ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg
     4801 gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag
     4861 gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca
     4921 actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg
     4981 agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct
     5041 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     5101 gcaaagaatt gtaatacgac tcactatagg gcgaattcgg atccaccacc atggagcgag
     5161 ctgagagctc gagtacagaa cctgctaagg ccatcaaacc tattgatcgg aagtcagtcc
     5221 atcagatttg ctctgggcag gtggtactga gtctaagcac tgcggtaaag gagttagtag
     5281 aaaacagtct ggatgctggt gccactaata ttgatctaaa gcttaaggac tatggagtgg
     5341 atcttattga agtttcagac aatggatgtg gggtagaaga agaaaacttc gaaggcttaa
     5401 ctctgaaaca tcacacatct aagattcaag agtttgccga cctaactcag gttgaaactt
     5461 ttggctttcg gggggaagct ctgagctcac tttgtgcact gagcgatgtc accatttcta
     5521 cctgccacgc atcggcgaag gttggaactc gactgatgtt tgatcacaat gggaaaatta
     5581 tccagaaaac cccctacccc cgccccagag ggaccacagt cagcgtgcag cagttatttt
     5641 ccacactacc tgtgcgccat aaggaatttc aaaggaatat taagaaggag tatgccaaaa
     5701 tggtccaggt cttacatgca tactgtatca tttcagcagg catccgtgta agttgcacca
     5761 atcagcttgg acaaggaaaa cgacagcctg tggtatgcac aggtggaagc cccagcataa
     5821 aggaaaatat cggctctgtg tttgggcaga agcagttgca aagcctcatt ccttttgttc
     5881 agctgccccc tagtgactcc gtgtgtgaag agtacggttt gagctgttcg gatgctctgc
     5941 ataatctttt ttacatctca ggtttcattt cacaatgcac gcatggagtt ggaaggagtt
     6001 caacagacag acagtttttc tttatcaacc ggcggccttg tgacccagca aaggtctgca
     6061 gactcgtgaa tgaggtctac cacatgtata atcgacacca gtatccattt gttgttctta
     6121 acatttctgt tgattcagaa tgcgttgata tcaatgttac tccagataaa aggcaaattt
     6181 tgctacaaga ggaaaagctt ttgttggcag ttttaaagac ctctttgata ggaatgtttg
     6241 atagtgatgt caacaagcta aatgtcagtc agcagccact gctggatgtt gaaggtaact
     6301 taataaaaat gcatgcagcg gatttggaaa agcccatggt agaaaagcag gatcaatccc
     6361 cttcattaag gactggagaa gaaaaaaaag acgtgtccat ttccagactg cgagaggcct
     6421 tttctcttcg tcacacaaca gagaacaagc ctcacagccc aaagactcca gaaccaagaa
     6481 ggagccctct aggacagaaa aggggtatgc tgtcttctag cacttcaggt gccatctctg
     6541 acaaaggcgt cctgagacct cagaaagagg cagtgagttc cagtcacgga cccagtgacc
     6601 ctacggacag agcggaggtg gagaaggact cggggcacgg cagcacttcc gtggattctg
     6661 aggggttcag catcccagac acgggcagtc actgcagcag cgagtatgcg gccagctccc
     6721 caggggacag gggctcgcag
//
