LOCUS       sequence-419421-        6733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generation of Bacmid for insect cell expression of C17orf53 
            C-terminally fused to FLAG-Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6733)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac1-C17orf53-FLAG
FEATURES             Location/Qualifiers
     source          1..6733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(98..117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     307..328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        491..595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(596..664,665..1456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(814..833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1627..2215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2116..2135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2369..2386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2520..2744
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2811..3344)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3533..3538,3539..3555,3556..3561))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3913..4004
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3929..3946
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             6009..6032
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6232..6366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6269..6288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6323..6342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6395..6560
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
       61 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      361 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      421 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
      481 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      541 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      601 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      661 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      721 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      781 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      841 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      901 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      961 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1021 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1081 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1141 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1201 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1261 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1321 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1381 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1441 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1501 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1561 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1621 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1681 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1741 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1801 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1861 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1921 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1981 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2041 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2101 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2161 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2221 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2281 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2341 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2401 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc
     2461 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt
     2521 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     2581 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     2641 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     2701 tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat
     2761 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg
     2821 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga
     2881 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg
     2941 ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg
     3001 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga
     3061 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat
     3121 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg
     3181 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg
     3241 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta
     3301 gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa
     3361 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     3421 tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
     3481 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg
     3541 aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca
     3601 cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg
     3661 caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa
     3721 ggtgctgtgc acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg
     3781 cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga
     3841 gcatcgtttg ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg
     3901 gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat
     3961 tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat
     4021 accgtcccac catcgggcgc ggatcccggt ccgaagcgcg cggaattctc atggcgtgca
     4081 gtttgcagaa gctgtttgct gtggaagagg agtttgaaga tgaggatttc ttgtctgctg
     4141 tggaggatgc agagaaccgg tttactggct cactgcctgt gaatgctggg cgcctgagac
     4201 ctgtctcttc taggccacag gagactgtgc aggcacagtc ctccaggctg ctgctgttac
     4261 accccactgc tccctcagag gctttgggcc tgccagactt ggacctctgc ctccctgcct
     4321 ccagcacgcc cagtgctgac agccgtccat catgcatagg agcagctccc ctaaggcctg
     4381 tctctacttc cagcagctgg attggcaatc agagaagagt gacagtgaca gaagtgctca
     4441 gagagacagc aagacctcag tcctcagcct tacaccccct actcaccttt gagagccaac
     4501 agcagcaagt tggtggcttt gaggggcctg aacaagacga atttgataaa gtcctggcaa
     4561 gcatggagtt ggaggagcct ggcatggagc tggaatgtgg agtcagcagt gaggccatac
     4621 caatcctgcc tgcccagcag cgggagggtt cagtattggc taaaaaagcc cgggtagttg
     4681 atctgagtgg atcttgccag aaggggcctg tgcctgccat ccacaaagcg ggtatcatgt
     4741 ccgcccagga tgagtctcta gatcctgtca tccaatgtag gactccacga ccccccttga
     4801 gacctggtgc tgtgggtcac cttcctgttc caactgcctt aacagttccc actcagcaac
     4861 tccactggga agtctgtccg caacgctccc ctgttcaagc acttcagcct ctccaagctg
     4921 ctagagggac cattcagagc agccctcaaa atcgtttccc ttgtcagcca ttccagtctc
     4981 caagttcctg gttaagtggc aaagctcatt tacccagacc tcgaactccc aactcaagct
     5041 gttctactcc ctcaaggact agctctggat tatttcctcg gataccctta caaccgcaag
     5101 ctccagtgtc ttccattggg tctcctgttg gtaccccaaa aggtccccag ggagctctgc
     5161 agacacccat agtcaccaac cacctggtgc agctagtcac tgctgccagc cggacacccc
     5221 agcagcccac ccatccctcc acccgagcca aaactcgccg tttccctggc ccagctggga
     5281 tcctgcctca ccagcagagt gggagaagtc tggaggacat catggtttcc gcgccccaaa
     5341 ctccaaccca tggtgctctg gctaaattcc agacagagat tgttgctagt tcccaggcat
     5401 ctgtggagga ggattttggg cgagggccct ggctgaccat gaaatccacg ctaggcctgg
     5461 atgagagaga ccctagctgc ttcctctgta cctacagcat tgtcatggtg ctgcgcaagg
     5521 cagccctgaa gcagcttcct aggaacaagg tccccaacat ggcggtgatg atcaagtccc
     5581 tgactcggag cacaatggac gccagtgtgg ttttcaagga ccccacggga gagatgcagg
     5641 ggacggtgca caggttgctg ctggagacgt gccagaatga gctgaagcct ggctcagtgc
     5701 tgctgctgaa gcagattgga gtgttttctc cttcacttcg aaatcactac ctcaacgtga
     5761 cacccaacaa cctggtccat atttacagcc cggattctgg ggatgggagc ttcctcaagc
     5821 catctcagcc cttccccaag gattcaggga gcttccagca tgatgtggct gcaaagcccg
     5881 agaaaggctt cagaacagca cagaacctag aggcagaggc gtcccctgag gaagaactcc
     5941 cagaagcaga tgacctggat ggactcctga gtgagcttcc tgaagacttc ttctgtggga
     6001 ccagtagtga ctacaaagac gatgacgaca agtgagtcga cgagctcact agtcgcggcc
     6061 gctttcgaat ctagagcctg cagtctcgag gcatgcggta ccaagcttgt cgagaagtac
     6121 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     6181 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6241 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6301 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6361 tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca
     6421 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     6481 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     6541 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
     6601 cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga
     6661 aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat
     6721 ttgcagcctg aat
//
