LOCUS       sequence-419422-        6708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generation of Bacmid for insect cell expression of C17orf53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6708)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFastBac1-C17orf53
FEATURES             Location/Qualifiers
     source          1..6708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(77..96)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     286..307
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        470..574
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(575..643,644..1435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(793..812)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1606..2194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2095..2114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2348..2365
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2499..2723
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2790..3323)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3512..3517,3518..3534,3535..3540))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3892..3983
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3908..3925
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    6186..6320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6223..6242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6277..6296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6349..6514
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      join(6698..6708,1..444)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
       61 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      121 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      181 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      241 tagacggttt ttcgcccttt gacgttggag tccacgttct taatagtgga ctcttgttcc
      301 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      361 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      421 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      721 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      901 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      961 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1021 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1081 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1141 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1201 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1261 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1321 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1381 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1441 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1741 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2221 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2281 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2341 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2401 tacgcatctg tgcggtattt cacaccgcat agaccagccg cgtaacctgg caaaatcggt
     2461 tacggttgag taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt
     2521 aaactgaaca aaatagatct aaactatgac aataaagtct taaactagac agaatagttg
     2581 taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa
     2641 agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca
     2701 agggcatggt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc
     2761 gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag
     2821 tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg
     2881 taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttgaggaga
     2941 ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag
     3001 atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca
     3061 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
     3121 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
     3181 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
     3241 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
     3301 acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     3361 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
     3421 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     3481 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
     3541 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
     3601 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
     3661 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
     3721 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
     3781 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga
     3841 ctctagctat agttctagtg gttggctacg tatactccgg aatattaata gatcatggag
     3901 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
     3961 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
     4021 gatcccggtc cgaagcgcgc ggaattctat ggcgtgcagt ttgcagaagc tgtttgctgt
     4081 ggaagaggag tttgaagatg aggatttctt gtctgctgtg gaggatgcag agaaccggtt
     4141 tactggctca ctgcctgtga atgctgggcg cctgagacct gtctcttcta ggccacagga
     4201 gactgtgcag gcacagtcct ccaggctgct gctgttacac cccactgctc cctcagaggc
     4261 tttgggcctg ccagacttgg acctctgcct ccctgcctcc agcacgccca gtgctgacag
     4321 ccgtccatca tgcataggag cagctcccct aaggcctgtc tctacttcca gcagctggat
     4381 tggcaatcag agaagagtga cagtgacaga agtgctcaga gagacagcaa gacctcagtc
     4441 ctcagcctta caccccctac tcacctttga gagccaacag cagcaagttg gtggctttga
     4501 ggggcctgaa caagacgaat ttgataaagt cctggcaagc atggagttgg aggagcctgg
     4561 catggagctg gaatgtggag tcagcagtga ggccatacca atcctgcctg cccagcagcg
     4621 ggagggttca gtattggcta aaaaagcccg ggtagttgat ctgagtggat cttgccagaa
     4681 ggggcctgtg cctgccatcc acaaagcggg tatcatgtcc gcccaggatg agtctctaga
     4741 tcctgtcatc caatgtagga ctccacgacc ccccttgaga cctggtgctg tgggtcacct
     4801 tcctgttcca actgccttaa cagttcccac tcagcaactc cactgggaag tctgtccgca
     4861 acgctcccct gttcaagcac ttcagcctct ccaagctgct agagggacca ttcagagcag
     4921 ccctcaaaat cgtttccctt gtcagccatt ccagtctcca agttcctggt taagtggcaa
     4981 agctcattta cccagacctc gaactcccaa ctcaagctgt tctactccct caaggactag
     5041 ctctggatta tttcctcgga tacccttaca accgcaagct ccagtgtctt ccattgggtc
     5101 tcctgttggt accccaaaag gtccccaggg agctctgcag acacccatag tcaccaacca
     5161 cctggtgcag ctagtcactg ctgccagccg gacaccccag cagcccaccc atccctccac
     5221 ccgagccaaa actcgccgtt tccctggccc agctgggatc ctgcctcacc agcagagtgg
     5281 gagaagtctg gaggacatca tggtttccgc gccccaaact ccaacccatg gtgctctggc
     5341 taaattccag acagagattg ttgctagttc ccaggcatct gtggaggagg attttgggcg
     5401 agggccctgg ctgaccatga aatccacgct aggcctggat gagagagacc ctagctgctt
     5461 cctctgtacc tacagcattg tcatggtgct gcgcaaggca gccctgaagc agcttcctag
     5521 gaacaaggtc cccaacatgg cggtgatgat caagtccctg actcggagca caatggacgc
     5581 cagtgtggtt ttcaaggacc ccacgggaga gatgcagggg acggtgcaca ggttgctgct
     5641 ggagacgtgc cagaatgagc tgaagcctgg ctcagtgctg ctgctgaagc agattggagt
     5701 gttttctcct tcacttcgaa atcactacct caacgtgaca cccaacaacc tggtccatat
     5761 ttacagcccg gattctgggg atgggagctt cctcaagcca tctcagccct tccccaagga
     5821 ttcagggagc ttccagcatg atgtggctgc aaagcccgag aaaggcttca gaacagcaca
     5881 gaacctagag gcagaggcgt cccctgagga agaactccca gaagcagatg acctggatgg
     5941 actcctgagt gagcttcctg aagacttctt ctgtgggacc agtagttgag tcgacgagct
     6001 cactagtcgc ggccgctttc gaatctagag cctgcagtct cgaggcatgc ggtaccaagc
     6061 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     6121 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     6181 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6301 tatcttatca tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt
     6361 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     6421 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     6481 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     6541 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     6601 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     6661 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgt
//
