LOCUS       sequence-419425-        6597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of the N-terminal amino acids 1-241 of human 
            C17orf53 N-terminally fused to Profinity eXact-Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6597)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPAL7-C17orf53_1-241
FEATURES             Location/Qualifiers
     source          1..6597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    32..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             71..93
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1110..1128)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1124..1171
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1346..1351,1352..1368,1369..1374))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1401..1419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1487..1591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1592..1660,1661..2452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1810..1829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2623..3211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3112..3131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3365..3382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3397..3536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3462..3481)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3622..3644
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3638..3829)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5141..6223)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6185..6204
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6224..6301)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6507..6526
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
       61 tagaaataat tttgtttaac tttaagaagg agatatacat atgggaggga aatcaaacgg
      121 ggaaaagaaa tatattgtcg ggttcaaaca gggctttaag agctgcgcta agaaggagga
      181 tgtcatttct gaaaaaggcg ggaaactcca aaagtgcttc aaatatgtag acgcagctag
      241 cgctacatta aacgaaaaag ctgtagaaga attgaaaaaa gatccgagcg tcgcgtacgt
      301 agaagaagac aagctcttca aagctttgat ggcgtgcagt ttgcagaagc tgtttgctgt
      361 ggaagaggag tttgaagatg aggatttctt gtctgctgtg gaggatgcag agaaccggtt
      421 tactggctca ctgcctgtga atgctgggcg cctgagacct gtctcttcta ggccacagga
      481 gactgtgcag gcacagtcct ccaggctgct gctgttacac cccactgctc cctcagaggc
      541 tttgggcctg ccagacttgg acctctgcct ccctgcctcc agcacgccca gtgctgacag
      601 ccgtccatca tgcataggag cagctcccct aaggcctgtc tctacttcca gcagctggat
      661 tggcaatcag agaagagtga cagtgacaga agtgctcaga gagacagcaa gacctcagtc
      721 ctcagcctta caccccctac tcacctttga gagccaacag cagcaagttg gtggctttga
      781 ggggcctgaa caagacgaat ttgataaagt cctggcaagc atggagttgg aggagcctgg
      841 catggagctg gaatgtggag tcagcagtga ggccatacca atcctgcctg cccagcagcg
      901 ggagggttca gtattggcta aaaaagcccg ggtagttgat ctgagtggat cttgccagaa
      961 ggggcctgtg cctgccatcc acaaagcggg tatcatgtcc gcccaggatg agtctctaga
     1021 tcctgtcatc caatgtagga ctccacgacc ctgagaattc ctcgaggcgg ccgcataagc
     1081 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1141 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
     1201 cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg
     1261 gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag
     1321 caatttaact gtgataaact accgcattaa agcctatcga tgataagctg tcaaacatga
     1381 gatttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1441 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1501 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1561 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1621 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1681 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1741 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1801 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     1861 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1921 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1981 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2041 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2101 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     2161 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2221 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2281 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2341 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2401 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2461 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2521 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2761 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3361 gcgcagcgag tcagtgagcg aggaagcgga acagcgcctg atgcggtatt ttctccttac
     3421 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     3481 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     3541 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3601 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3661 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     3721 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     3781 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     3841 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     3901 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     3961 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     4021 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     4081 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     4141 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     4201 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     4261 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     4321 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     4381 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     4441 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     4501 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     4561 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     4621 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     4681 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     4741 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     4801 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     4861 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     4921 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     4981 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     5041 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     5101 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5221 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     5281 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     5341 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     5401 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     5461 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     5521 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     5581 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     5641 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     5701 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     5761 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     5821 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     5881 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     5941 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     6001 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     6061 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     6121 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     6181 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     6241 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     6301 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     6361 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     6421 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     6481 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     6541 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcg
//
