LOCUS       sequence-419429-        8092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human C17orf53 C-terminally fused to 
            mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8092)
  TITLE     Pospiech Lab Plasmids 2023
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-C17orf53-mCer
FEATURES             Location/Qualifiers
     source          1..8092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2924..2933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2930..2932,2933..2935,2936..3649)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2975..2996)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3236..3255)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3583..3604
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(3659..3677)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3660..3677)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3697..3714)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3703..3927
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3973..4401
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4060..4079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4270..4291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4410..4430)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4415..4744
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4595..4730
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4657..4676
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4811..5605
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4865..4884)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5475..5494
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5779..5900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5816..5835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5870..5889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5949..5965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5949..5965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5962..5984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5973..5989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5997..6003,6004..6021,6022..6027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6042..6063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6180..6197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6351..6939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6431..6450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7110..7901,7902..7970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7733..7752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7971..8075)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
       61 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
      121 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
      181 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      241 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
      841 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      901 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattctat
      961 ggcgtgcagt ttgcagaagc tgtttgctgt ggaagaggag tttgaagatg aggatttctt
     1021 gtctgctgtg gaggatgcag agaaccggtt tactggctca ctgcctgtga atgctgggcg
     1081 cctgagacct gtctcttcta ggccacagga gactgtgcag gcacagtcct ccaggctgct
     1141 gctgttacac cccactgctc cctcagaggc tttgggcctg ccagacttgg acctctgcct
     1201 ccctgcctcc agcacgccca gtgctgacag ccgtccatca tgcataggag cagctcccct
     1261 aaggcctgtc tctacttcca gcagctggat tggcaatcag agaagagtga cagtgacaga
     1321 agtgctcaga gagacagcaa gacctcagtc ctcagcctta caccccctac tcacctttga
     1381 gagccaacag cagcaagttg gtggctttga ggggcctgaa caagacgaat ttgataaagt
     1441 cctggcaagc atggagttgg aggagcctgg catggagctg gaatgtggag tcagcagtga
     1501 ggccatacca atcctgcctg cccagcagcg ggagggttca gtattggcta aaaaagcccg
     1561 ggtagttgat ctgagtggat cttgccagaa ggggcctgtg cctgccatcc acaaagcggg
     1621 tatcatgtcc gcccaggatg agtctctaga tcctgtcatc caatgtagga ctccacgacc
     1681 ccccttgaga cctggtgctg tgggtcacct tcctgttcca actgccttaa cagttcccac
     1741 tcagcaactc cactgggaag tctgtccgca acgctcccct gttcaagcac ttcagcctct
     1801 ccaagctgct agagggacca ttcagagcag ccctcaaaat cgtttccctt gtcagccatt
     1861 ccagtctcca agttcctggt taagtggcaa agctcattta cccagacctc gaactcccaa
     1921 ctcaagctgt tctactccct caaggactag ctctggatta tttcctcgga tacccttaca
     1981 accgcaagct ccagtgtctt ccattgggtc tcctgttggt accccaaaag gtccccaggg
     2041 agctctgcag acacccatag tcaccaacca cctggtgcag ctagtcactg ctgccagccg
     2101 gacaccccag cagcccaccc atccctccac ccgagccaaa actcgccgtt tccctggccc
     2161 agctgggatc ctgcctcacc agcagagtgg gagaagtctg gaggacatca tggtttccgc
     2221 gccccaaact ccaacccatg gtgctctggc taaattccag acagagattg ttgctagttc
     2281 ccaggcatct gtggaggagg attttgggcg agggccctgg ctgaccatga aatccacgct
     2341 aggcctggat gagagagacc ctagctgctt cctctgtacc tacagcattg tcatggtgct
     2401 gcgcaaggca gccctgaagc agcttcctag gaacaaggtc cccaacatgg cggtgatgat
     2461 caagtccctg actcggagca caatggacgc cagtgtggtt ttcaaggacc ccacgggaga
     2521 gatgcagggg acggtgcaca ggttgctgct ggagacgtgc cagaatgagc tgaagcctgg
     2581 ctcagtgctg ctgctgaagc agattggagt gttttctcct tcacttcgaa atcactacct
     2641 caacgtgaca cccaacaacc tggtccatat ttacagcccg gattctgggg atgggagctt
     2701 cctcaagcca tctcagccct tccccaagga ttcagggagc ttccagcatg atgtggctgc
     2761 aaagcccgag aaaggcttca gaacagcaca gaacctagag gcagaggcgt cccctgagga
     2821 agaactccca gaagcagatg acctggatgg actcctgagt gagcttcctg aagacttctt
     2881 ctgtgggacc agtagtctcg agcatgcatc tagaagatcc atcgccacca tggtgagcaa
     2941 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3001 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3061 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3121 cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3181 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3241 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3301 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3361 caacgccatc agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     3421 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3481 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3541 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3601 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag ggccctattc
     3661 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     3721 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3781 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3841 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3901 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     3961 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4021 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4081 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4141 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4201 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4261 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4321 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4381 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4441 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4501 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4561 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4621 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4681 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4741 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4801 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4861 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4921 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4981 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5041 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5101 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5161 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5221 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5281 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5341 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5401 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5461 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5521 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5581 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5641 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5701 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5761 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5881 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5941 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6001 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6061 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6121 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6181 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6241 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6301 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6361 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6421 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6481 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6541 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6601 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6661 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6721 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6781 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6841 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6901 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6961 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7021 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7081 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7141 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7201 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7261 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7321 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7381 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7441 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7501 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7561 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7621 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7681 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7741 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7801 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7861 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7921 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7981 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8041 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa ag
//
